25º Congresso Brasileiro de Microbiologia

25º Congresso Brasileiro de Microbiologia

ResumoID:2474-1

Área: Fermentação e Biotecnologia ( Divisão J )

STUDY OF RECOMBINANT PLASMID STABILITY TO KANAMYCIN RESISTENCE IN BIOREACTOR

Natasha Kuniechick (FFARM); Rafael Munareto do Nascimento (FFARM); Thiago Milech Assunção (INCTB); Cláudia Paiva Nunes (INCTB); Jocelei Chies (4G P&D); Luiz Augusto Basso (INCTB); Diógenes Santiago Santos (INCTB)

Resumo

Escherichia coli is one of the most widely used hosts for production of heterologous proteins. One example of these recombinant proteins is the human growth hormone (hGH), a pituitary-derived polypeptide with a wide range of biological functions including protein synthesis, cell proliferation and therapeutic applications in different treatments.

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The plasmid stability test determines what proportion of the cells maintain the target plasmid and the ability to express the target protein.

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In order to measure the growth rate, cells harboring plasmids were used to estimate the number of generations that were used in the experimental plots, to define a number of generations used for the analyzes.

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After 32 hours of cultivation with IPTG induction, the plasmid presented a high stability in both media, with or without kanamycin, indicating a good resistance to this antibiotic with protein expression until the end of cultivation.

Palavras-chave: Bioreactor, Plasmid, Stability

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