ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:2355-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Microbiologia Veterinária ( Divisão G )</b><p align=justify><strong>PERSISTENSE OF <EM>BRUCELLA ABORTUS </EM>RB51 VACCINE STRAIN IN MILK OF VACCINATED ADULT CATTLE</strong></p><p align=justify><b>Karina Leite Miranda </b> (<i>UFMG</i>); <b>Fernando Padilla Poester </b> (<i>UFMG</i>); <b><u>Elaine Maria Seles Dorneles </u></b> (<i>UFMG</i>); <b>Thiago Magalhães Resende </b> (<i>UFMG</i>); <b>Adil Knackfuss Vaz </b> (<i>UDESC</i>); <b>Sandra Maria Ferraz </b> (<i>UDESC</i>); <b>Andrey Pereira Lage </b> (<i>UFMG</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2><P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: justify; mso-layout-grid-align: none"><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">Brucellosis caused by </SPAN><I><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPS-ItalicMT; mso-bidi-font-family: TimesNewRomanPS-ItalicMT; mso-ansi-language: EN-US">Brucella abortus </SPAN></I><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">is a chronic disease of cattle of worldwide economic</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">and public health importance resulting in abortion and infertility. Brucellosis in cattle in</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><?xml:namespace prefix = st1 ns = "urn:schemas-microsoft-com:office:smarttags" /><st1:country-region w:st="on"><st1:place w:st="on"><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">Brazil</SPAN></st1:place></st1:country-region><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US"> is widespread in the country, although it has an uneven distribution with areas with</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">very low and others with high prevalences of cattle and herd infections. Voluntary vaccination</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">of adult animals with RB51 is approved by MAPA as additional control strategy. The aim of this study was to evaluate the shedding of </SPAN><I><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPS-ItalicMT; mso-bidi-font-family: TimesNewRomanPS-ItalicMT; mso-ansi-language: EN-US">B. abortus </SPAN></I><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">in the milk of cows vaccinated with RB51. Nine cows, vaccinated with S19 as calf and other nine cows, not</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">vaccinated with S19 were used. The animals were vaccinated 30 to 60 days after parturition</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">with 1.3 x 10</SPAN><SUP><SPAN lang=EN-US style="FONT-SIZE: 8pt; FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">10 </SPAN></SUP><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">CFU of </SPAN><I><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPS-ItalicMT; mso-bidi-font-family: TimesNewRomanPS-ItalicMT; mso-ansi-language: EN-US">B. abortus </SPAN></I><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">strain RB51 subcutaneously on day 0 of the experiment.</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">Milk samples were collected on days 1, 2, 3, 4, 7, and weekly up to day 63 after vaccination.</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">The samples were manually collected from all quarters, mixed and stored at -20ºC. Samples</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">were thawed and centrifuged at 2500 x g, for 15 minutes. The skim milk was discarded and</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">the cream and deposit were mixed and immediately spread onto tryptose agar plates</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">with antibiotics (Farrell s supplement) in duplicates. Moreover, 1 mL of each mixture was inoculated in 9 mL of enrichment media (tryptose broth with Farrell s sellective supplement) and incubated into 5% CO</SPAN><SPAN lang=EN-US style="FONT-SIZE: 8pt; FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">2 </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">at 37ºC for 5 days, and then inoculated onto tryptose agar with antibiotics. All plates were incubated into 5% CO</SPAN><SPAN lang=EN-US style="FONT-SIZE: 8pt; FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">2 </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">at 37ºC for 9 days. Samples were also tested by genera specific PCR</SPAN><SPAN lang=EN-US style="FONT-SIZE: 8pt; FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">. </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">No </SPAN><I><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPS-ItalicMT; mso-bidi-font-family: TimesNewRomanPS-ItalicMT; mso-ansi-language: EN-US">B. abortus </SPAN></I><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">was isolated from any sample tested. Only one sample, collected on the first day after RB51 vaccination, from a B19-calf vaccinated cow, was positive by PCR. In conclusion the spread of RB51 by milk seems not to be a public health problem. Nevertheless the pasteurization of the milk from cows recently vaccinated with RB51 is highly</SPAN><SPAN lang=EN-US style="FONT-FAMILY: ArialMT; mso-bidi-font-family: ArialMT; mso-ansi-language: EN-US"> </SPAN><SPAN lang=EN-US style="FONT-FAMILY: TimesNewRomanPSMT; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: EN-US">recommended. Agradecimentos: Fapemig, CNPq, Capes e FEP-MVZ Coordenação financeira.</SPAN></P></font></p><br><b>Palavras-chave: </b>&nbsp;brucelosis, cattle, milk, RB51, vaccine</td></tr></table></tr></td></table></body></html>