25º Congresso Brasileiro de Microbiologia

25º Congresso Brasileiro de Microbiologia

ResumoID:2188-1

Área: Ecologia Microbiana ( Divisão I )

MICROBIAL ECOLOGY AND APPLIED MICROBIOLOGY OF SULPHIDIC SUBMARINE CAVES

Francesco Canganella (UNITUS); Giovanna Bianconi (UNITUS)

Resumo

Extremophilic microorganisms are presently investigated and described in terms of taxonomy, phylogeny and potentially biotechnological applications.

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However the biota is primarily characterized by prokaryotes (both autotrophic and heterotrophic) adapted to anoxic condition and capable to form extensive biofilms on the rocky surfaces. The absence of solar irradiation, the chemo-physical traits and the fact that prokaryotes are the only living forms in specific areas of these cavities well justify the definition of such habitats as extreme.

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Microbial strains isolated from these habitats are taxonomically described by traditional and molecular techniques, including SDS-PAGE and DGGE; moreover hydrolytic enzymes as well as bioactive molecules including biosurfactants are screened and characterized.

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Samples were used to inoculate enrichment media in order to investigate the most representative morphologies. Further and extensive studies were carried out by SEM and TEM analyses to determine the structural features of bacteria. The physiological patterns of microbial populations along the water column and in the bottom sediments were described by the BIOLOG system. Moreover the molecular biodiversity of the ecosystem was investigated in order to describe the distribution of microorganisms and their putative physiological roles.

Palavras-chave: Submarine caves, microbial ecology, biodiversity, sulphidic

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