| Área: Microbiologia Geral ( Divisão H ) LEPTOSPIRA SEROVAR IDENTIFICATION BY PCR-RFLP OF RPOB GENE
Lenice Roteia Cardoso Jung (UFMG-ICB); Maria Rosa Quaresma Bomfim (UFMG-ICB); João Luiz Silva Moreira (UFMG-ICB); Erna Geessien Kroon (UFMG-ICB); Regina Maria Nardi Drummond (UFMG-ICB); Alvaro Cantini Nunes (UFMG-ICB)
ResumoABSTRACT 㰀倀 挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀 猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀 挀洀 挀洀 瀀琀 㔀㐀瀀琀㬀 吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀 樀甀猀琀椀昀礀∀㸀㰀匀倀䄀一 氀愀渀最㴀䔀一ⴀ唀匀 猀琀礀氀攀㴀∀洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀㰀漀㨀瀀㸀☀渀戀猀瀀㬀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀
Identification of Leptospira species is important because leptospirosis is an infectious disease that has reemerging in urban centers throughout the world. To date, observation of these bacteria requires dark-field microscopy, and their identification has long relied on serotyping. The development of alternative tools for identification of Leptospira serovars is required, to circumvent a major risk of contamination during sample cultivation. The need for rapid and specific characterization of leptospires has stimulated the use of new molecular biological tests. The aim of study was to investigate the possible identification of some serovars by RFLP typing 㰀倀 挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀 猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀 挀洀 挀洀 瀀琀㬀 䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀 㔀 ─㬀 吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀 樀甀猀琀椀昀礀∀㸀㰀䈀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 渀漀爀洀愀氀∀㸀㰀匀倀䄀一 氀愀渀最㴀䔀一ⴀ唀匀 猀琀礀氀攀㴀∀洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀䴀愀琀攀爀椀愀氀 愀渀搀 䴀攀琀栀漀搀猀㨀㰀⼀匀倀䄀一㸀㰀⼀䈀㸀㰀匀倀䄀一 氀愀渀最㴀䔀一ⴀ唀匀 猀琀礀氀攀㴀∀洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀 䐀一䄀 眀愀猀 攀砀琀爀愀挀琀攀搀 昀爀漀洀 㘀㠀 㰀䤀㸀䰀攀瀀琀漀猀瀀椀爀愀㰀⼀䤀㸀 猀攀爀漀瘀愀爀猀Ⰰ 戀攀氀漀渀最椀渀最 漀昀 搀椀昀昀攀爀攀渀琀 㰀匀倀䄀一㸀瀀愀琀栀漀最攀渀椀挀 愀渀搀 猀愀瀀爀漀瀀栀礀琀椀挀 猀瀀攀挀椀攀猀⸀ 㰀⼀匀倀䄀一㸀倀漀氀礀洀攀爀愀猀攀 挀栀愀椀渀 爀攀愀挀琀椀漀渀 ⠀倀䌀刀⤀ 愀猀猀愀礀猀 㰀匀倀䄀一 猀琀礀氀攀㴀∀䌀伀䰀伀刀㨀 戀氀愀挀欀∀㸀眀攀爀攀 瀀攀爀昀漀爀洀攀搀 甀猀椀渀最 㰀䈀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 渀漀爀洀愀氀∀㸀猀㰀⼀䈀㸀㰀匀倀䄀一 猀琀礀氀攀㴀∀䘀伀一吀ⴀ圀䔀䤀䜀䠀吀㨀 渀漀爀洀愀氀㬀 洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 戀漀氀搀∀㸀瀀攀挀椀昀椀挀 瀀爀椀洀攀爀猀㰀⼀匀倀䄀一㸀㰀䈀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 渀漀爀洀愀氀∀㸀 㰀⼀䈀㸀搀攀猀椀最渀攀搀 㰀⼀匀倀䄀一㸀琀漀 愀洀瀀氀椀昀礀 愀 昀爀愀最洀攀渀琀 漀昀 琀栀攀 最攀渀攀 挀漀搀椀渀最 琀栀攀 㰀匀倀䄀一㸀戀攀琀愀 猀甀戀甀渀椀琀 漀昀 㰀匀倀䄀一 猀琀礀氀攀㴀∀䘀伀一吀ⴀ圀䔀䤀䜀䠀吀㨀 渀漀爀洀愀氀㬀 洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 戀漀氀搀∀㸀刀一䄀 瀀漀氀礀洀攀爀愀猀攀㰀⼀匀倀䄀一㸀 ⠀㰀⼀匀倀䄀一㸀爀瀀漀䈀⤀⸀ 吀栀攀 攀渀稀礀洀愀琀椀挀 搀椀最攀猀琀椀漀渀 眀愀猀 瀀攀爀昀漀爀洀攀搀 甀猀椀渀最 琀栀攀 攀渀稀礀洀攀猀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀吀愀焀㰀⼀䤀㸀䤀Ⰰ 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀匀愀甀㰀⼀䤀㸀㌀䄀䤀Ⰰ 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䴀猀攀㰀⼀䤀㸀䤀Ⰰ 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䴀猀氀㰀⼀䤀㸀䤀 愀渀搀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䔀挀漀㰀⼀䤀㸀一䤀Ⰰ 瀀爀攀瘀椀漀甀猀氀礀 猀攀氀攀挀琀攀搀 戀愀猀攀搀 椀渀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䔀砀瀀爀攀猀猀攀搀 匀攀焀甀攀渀挀攀 吀愀最猀㰀⼀䤀㸀 㰀匀倀䄀一 猀琀礀氀攀㴀∀䌀伀䰀伀刀㨀 戀氀愀挀欀∀㸀愀渀搀 渀甀挀氀攀漀琀椀搀攀 猀攀焀甀攀渀挀攀猀 愀瘀愀椀氀愀戀氀攀 椀渀 搀愀琀愀戀愀猀攀猀⸀ 吀栀攀 㰀匀倀䄀一 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 戀漀氀搀∀㸀爀攀猀琀爀椀挀琀椀漀渀㰀⼀匀倀䄀一㸀 㰀匀倀䄀一 猀琀礀氀攀㴀∀䘀伀一吀ⴀ圀䔀䤀䜀䠀吀㨀 渀漀爀洀愀氀㬀 洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 戀漀氀搀∀㸀昀爀愀最洀攀渀琀猀 眀攀爀攀 猀攀瀀愀爀愀琀攀搀㰀⼀匀倀䄀一㸀 椀渀 㰀匀倀䄀一 猀琀礀氀攀㴀∀䘀伀一吀ⴀ圀䔀䤀䜀䠀吀㨀 渀漀爀洀愀氀㬀 洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 戀漀氀搀∀㸀瀀漀氀礀愀挀爀礀氀愀洀椀搀攀 最攀氀猀㰀⼀匀倀䄀一㸀 愀渀搀 㰀匀倀䄀一 猀琀礀氀攀㴀∀䘀伀一吀ⴀ圀䔀䤀䜀䠀吀㨀 渀漀爀洀愀氀㬀 洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 戀漀氀搀∀㸀猀琀愀椀渀攀搀㰀⼀匀倀䄀一㸀㰀䈀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 渀漀爀洀愀氀∀㸀 㰀⼀䈀㸀眀椀琀栀 㰀匀倀䄀一 猀琀礀氀攀㴀∀䘀伀一吀ⴀ圀䔀䤀䜀䠀吀㨀 渀漀爀洀愀氀㬀 洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 戀漀氀搀∀㸀猀椀氀瘀攀爀㰀⼀匀倀䄀一㸀 渀椀琀爀愀琀攀⸀㰀⼀匀倀䄀一㸀 㰀䈀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 渀漀爀洀愀氀∀㸀刀攀猀甀氀琀猀㨀 㰀⼀䈀㸀圀攀 眀攀爀攀 愀戀氀攀 琀漀 搀椀昀昀攀爀攀渀琀椀愀琀攀 㔀㠀─ 漀昀 琀栀攀 猀攀爀漀瘀愀爀猀 琀攀猀琀攀搀⸀ 㰀匀倀䄀一 猀琀礀氀攀㴀∀䌀伀䰀伀刀㨀 戀氀愀挀欀∀㸀䠀漀眀攀瘀攀爀 ㈀㤀 猀攀爀漀瘀愀爀猀 昀爀漀洀 㤀 猀瀀攀挀椀攀猀 眀攀爀攀 渀漀琀 椀搀攀渀琀椀昀椀攀搀 礀攀琀Ⰰ 眀攀 猀攀氀攀挀琀 椀渀 搀愀琀愀戀愀猀攀Ⰰ 攀渀稀礀洀攀猀 琀栀愀琀 挀愀渀 椀搀攀渀琀椀昀礀 琀栀攀 瘀愀爀椀愀琀椀漀渀猀 椀渀 漀琀栀攀爀猀 最攀渀攀猀 氀椀欀攀㨀 䐀一䄀 䈀最礀爀愀猀攀 最攀渀攀Ⰰ 吀爀愀渀猀氀漀挀愀猀攀 瀀爀攀 ⴀ瀀爀漀琀攀椀渀 最攀渀攀 ⠀匀攀挀夀⤀Ⰰ 䤀猀漀挀椀琀爀愀琀攀 搀攀栀椀搀爀漀最攀渀愀猀攀 最攀渀攀 ⠀椀挀搀䄀⤀ 㰀⼀匀倀䄀一㸀愀渀搀 甀猀攀搀 昀漀爀 琀栀椀猀 瀀甀爀瀀漀猀攀㰀䈀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 渀漀爀洀愀氀∀㸀⸀ 䌀漀渀挀氀甀猀椀漀渀㰀⼀䈀㸀㨀 䈀礀 甀猀攀 漀昀㰀匀倀䄀一 猀琀礀氀攀㴀∀䌀伀䰀伀刀㨀 戀氀愀挀欀㬀 洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 戀漀氀搀∀㸀 爀攀猀琀爀椀挀琀椀漀渀 琀礀瀀椀渀最㰀⼀匀倀䄀一㸀Ⰰ 琀栀攀 瀀栀礀氀漀最攀渀攀琀椀挀 猀琀甀搀礀 漀昀 攀砀琀爀攀洀攀氀礀 昀愀猀琀椀搀椀漀甀猀 㰀䤀㸀䰀攀瀀琀漀猀瀀椀爀愀㰀⼀䤀㸀 猀瀀攀挀椀攀猀 挀愀渀 戀攀 挀愀爀爀椀攀搀 漀甀琀 眀椀琀栀漀甀琀 琀栀攀 渀攀攀搀 琀漀 挀甀氀琀甀爀攀 琀栀攀洀⸀ 䤀渀 愀搀搀椀琀椀漀渀Ⰰ 琀栀椀猀 琀攀挀栀渀椀焀甀攀 椀猀 愀 瀀爀漀洀椀猀椀渀最 琀漀漀氀 琀漀 攀瘀愀氀甀愀琀攀 最攀渀攀琀椀挀 搀椀瘀攀爀猀椀琀礀 漀昀 爀攀氀愀琀攀搀 猀琀爀愀椀渀猀 愀渀搀 洀愀礀 戀攀 甀猀攀昀甀氀 琀漀 搀椀昀昀攀爀攀渀琀椀愀琀攀 㰀䤀㸀䰀攀瀀琀漀猀瀀椀爀愀 㰀⼀䤀㸀猀攀爀漀瘀愀爀猀 眀椀琀栀椀渀 琀栀攀 猀瀀攀挀椀攀猀⸀ 㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀
㰀倀 挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀 猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀 挀洀 挀洀 瀀琀㬀 䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀 㔀 ─㬀 洀猀漀ⴀ漀甀琀氀椀渀攀ⴀ氀攀瘀攀氀㨀 㐀∀㸀㰀䈀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 渀漀爀洀愀氀∀㸀㰀匀倀䄀一 猀琀礀氀攀㴀∀䌀伀䰀伀刀㨀 戀氀愀挀欀∀㸀匀甀瀀瀀漀爀琀攀搀 戀礀㨀 䌀漀渀猀攀氀栀漀 一愀挀椀漀渀愀氀 搀攀 倀䔀匀儀唀䤀匀䄀 ⠀䌀一倀焀⤀ 愀渀搀 㰀⼀匀倀䄀一㸀㰀䄀 栀爀攀昀㴀∀栀琀琀瀀㨀⼀⼀眀眀眀⸀昀愀瀀攀洀椀最⸀戀爀⼀∀㸀㰀匀倀䄀一 猀琀礀氀攀㴀∀䌀伀䰀伀刀㨀 眀椀渀搀漀眀琀攀砀琀㬀 吀䔀堀吀ⴀ䐀䔀䌀伀刀䄀吀䤀伀一㨀 渀漀渀攀㬀 琀攀砀琀ⴀ甀渀搀攀爀氀椀渀攀㨀 渀漀渀攀∀㸀䘀甀渀搀愀漀 搀攀 䄀洀瀀愀爀漀 倀攀猀焀甀椀猀愀 搀漀 䔀猀琀愀搀漀 搀攀 䴀椀渀愀猀 䜀攀爀愀椀猀㰀⼀匀倀䄀一㸀㰀⼀䄀㸀 ⴀ䘀䄀倀䔀䴀䤀䜀㰀⼀䈀㸀㰀䈀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 渀漀爀洀愀氀∀㸀㰀匀倀䄀一 猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀䴀匀 刀攀昀攀爀攀渀挀攀 匀愀渀猀 匀攀爀椀昀✀Ⰰ✀猀愀渀猀ⴀ猀攀爀椀昀✀∀㸀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀䈀㸀㰀⼀倀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀倀愀氀愀瘀爀愀猀ⴀ挀栀愀瘀攀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀椀搀攀渀琀椀昀椀挀愀琀椀漀渀Ⰰ 最攀渀攀 爀瀀漀䈀Ⰰ 倀䌀刀ⴀ刀䘀䰀倀Ⰰ 猀攀爀漀瘀愀爀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀
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