<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font  size=1>ResumoID:2144-2</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Genética e Biologia Molecular ( Divisão N )</b><p align=justify><strong>਀  䴀伀䰀䔀䌀唀䰀䄀刀 䌀䰀伀一䤀一䜀Ⰰ 伀嘀䔀刀倀刀伀䐀唀䌀吀䤀伀一 䄀一䐀 䌀䠀䄀刀䄀䌀吀䔀刀䤀娀䄀吀䤀伀一 伀䘀 㰀匀倀䄀一 匀吀夀䰀䔀㴀∀䘀伀一吀ⴀ匀吀夀䰀䔀㨀 䤀吀䄀䰀䤀䌀㬀∀㸀䴀夀䌀伀䈀䄀䌀吀䔀刀䤀唀䴀 吀唀䈀䔀刀䌀唀䰀伀匀䤀匀 㰀⼀匀倀䄀一㸀䤀䴀倀 䐀䔀䠀夀䐀刀伀䜀䔀一䄀匀䔀 ⠀䔀䌀 ㄀⸀㄀⸀㄀⸀㈀　㔀⤀       
</strong></p><p align=justify><b><u>Thiago Milech de Assunção </u></b>  (<i>INCT-TB</i>); <b>Cristopher  Schneider </b>  (<i>INCT-TB</i>); <b>Luiz Augusto  Basso </b>  (<i>INCT-TB</i>); <b>Diógenes  Santiago Santos </b>  (<i>INCT-TB</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2>਀ 
<p class="MsoNormal" style="text-align: justify; line-height: 150%;"><i style=""><span style="font-size: 12pt; line-height: 150%;" lang="EN-US">Mycobacterium tuberculosis</span></i><span style="font-size: 12pt; line-height: 150%;" lang="EN-US">,਀琀栀攀 愀最攀渀琀 漀昀 栀甀洀愀渀 琀甀戀攀爀挀甀氀漀猀椀猀 ⠀吀䈀⤀Ⰰ 挀愀甀猀攀猀 愀戀漀甀琀 ㈀ 洀椀氀氀椀漀渀 搀攀愀琀栀猀 瀀攀爀 礀攀愀爀⸀ 䤀琀 
is estimated by the World Health Organization that one-third of the world’s਀瀀漀瀀甀氀愀琀椀漀渀 椀猀 椀渀昀攀挀琀攀搀 眀椀琀栀 㰀椀 猀琀礀氀攀㴀∀∀㸀䴀⸀ 
tuberculosis</i> in a latent form. Furthermore, the increasing emergence of਀洀甀氀琀椀搀爀甀最ⴀ爀攀猀椀猀琀愀渀琀 猀琀爀愀椀渀猀 栀愀猀 挀漀洀瀀氀椀挀愀琀攀搀 琀栀攀 琀爀攀愀琀洀攀渀琀 愀渀搀 挀漀渀琀爀漀氀 漀昀 吀䈀⸀ 䬀渀漀眀氀攀搀最攀 
of the mode of action and role of purine <i style="">de਀渀漀瘀漀㰀⼀椀㸀 瀀愀琀栀眀愀礀 攀渀稀礀洀攀猀 椀渀 㰀椀 猀琀礀氀攀㴀∀∀㸀䴀⸀ 
tuberculosis</i>, which are required for mycobacterial growth, could reveal new਀琀愀爀最攀琀猀 昀漀爀 琀栀攀 爀愀琀椀漀渀愀氀 搀攀猀椀最渀 漀昀 瀀漀琀攀渀琀 愀渀搀 猀攀氀攀挀琀椀瘀攀 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㈀瀀琀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀㔀　─㬀∀ 氀愀渀最㴀∀䔀一ⴀ唀匀∀㸀愀渀琀椀ⴀ吀䈀 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㈀瀀琀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀㔀　─㬀∀ 氀愀渀最㴀∀䔀一ⴀ唀匀∀㸀愀最攀渀琀猀 琀栀愀琀 洀椀最栀琀 戀攀 愀挀琀椀瘀攀 
against drug-resistant strains. Inosine monophosphate dehydrogenase (IMPDH)਀挀愀琀愀氀礀稀攀猀 琀栀攀 渀椀挀漀琀椀渀愀洀椀搀攀ⴀ愀搀攀渀椀渀攀 搀椀渀甀挀氀攀漀琀椀搀攀 ⠀一䄀䐀㰀猀甀瀀㸀⬀㰀⼀猀甀瀀㸀⤀ⴀ搀攀瀀攀渀搀攀渀琀 
oxidation of inosine 5’-monophosphate (IMP) to xanthosine 5’-monophosphate਀⠀堀䴀倀⤀⸀ 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㈀瀀琀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀㔀　─㬀∀ 氀愀渀最㴀∀䔀一ⴀ唀匀∀㸀䈀攀挀愀甀猀攀 
this enzyme acts on the synthesis of purine nucleotides at a critical step, it਀挀愀渀 戀攀 攀猀猀攀渀琀椀愀氀 昀漀爀 洀礀挀漀戀愀挀琀攀爀椀愀氀 最爀漀眀琀栀㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㈀瀀琀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀㔀　─㬀∀ 氀愀渀最㴀∀䔀一ⴀ唀匀∀㸀⸀ 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㈀瀀琀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀㔀　─㬀∀ 氀愀渀最㴀∀䔀一ⴀ唀匀∀㸀吀栀攀 最漀愀氀 漀昀 琀栀椀猀 眀漀爀欀 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㈀瀀琀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀㔀　─㬀∀ 氀愀渀最㴀∀䔀一ⴀ唀匀∀㸀椀猀 琀漀 猀琀甀搀礀 愀渀搀 甀渀搀攀爀猀琀愀渀搀 琀栀攀 欀椀渀攀琀椀挀猀 愀渀搀 
biochemistry of the recombinant IMPDH enzyme from <i style="">M. tuberculosis</i>. </span><span style="font-size: 12pt; line-height: 150%;" lang="EN-US">The complete DNA coding sequence of IMPDH਀⠀䔀䌀 ㄀⸀㄀⸀㄀⸀㈀　㔀⤀ 昀爀漀洀 㰀椀 猀琀礀氀攀㴀∀∀㸀䴀⸀ 琀甀戀攀爀挀甀氀漀猀椀猀㰀⼀椀㸀 
was obtained using genomic DNA in standard polymerase chain reaction (PCR) conditions.਀吀栀攀 愀洀瀀氀椀昀椀攀搀 昀爀愀最洀攀渀琀 眀愀猀 挀氀漀渀攀搀 椀渀琀漀 琀栀攀 瀀䌀刀ⴀ䈀氀甀渀琀 瘀攀挀琀漀爀 愀渀搀 猀甀戀挀氀漀渀攀搀 椀渀琀漀 
the <i><span style="">&nbsp;</span>Nde</i>I/<i>Hin</i>dIII਀瀀漀氀礀氀椀渀欀攀爀 爀攀最椀漀渀 漀昀 琀栀攀 瀀䔀吀ⴀ㈀㌀愀 ⠀⬀⤀ 瀀爀漀欀愀爀礀漀琀椀挀 攀砀瀀爀攀猀猀椀漀渀 瘀攀挀琀漀爀⸀ 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㈀瀀琀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀㔀　─㬀∀ 氀愀渀最㴀∀䔀一ⴀ唀匀∀㸀一甀挀氀攀漀琀椀搀攀 猀攀焀甀攀渀挀攀 漀昀 琀栀攀 
cloned fragment was determined by automated DNA sequencing. The recombinant਀瀀氀愀猀洀椀搀 眀愀猀 琀栀攀渀 猀甀戀樀攀挀琀攀搀 琀漀 搀椀昀昀攀爀攀渀琀 攀砀瀀爀攀猀猀椀漀渀 琀攀猀琀猀 椀渀 搀椀昀昀攀爀攀渀琀 㰀椀㸀䔀猀挀栀攀爀椀挀栀椀愀 
coli</i> strains. The enzyme was expressed in <i style="">E. coli</i> BL21 (DE3) cells in the soluble fraction, providing sufficient਀洀愀琀攀爀椀愀氀 昀漀爀 瀀甀爀椀昀椀挀愀琀椀漀渀 戀礀 䘀倀䰀䌀Ⰰ 戀椀漀挀栀攀洀椀猀琀爀礀 愀猀猀愀礀猀Ⰰ 搀攀琀攀爀洀椀渀愀琀椀漀渀 漀昀 椀琀猀 
three-dimensional structure and study of its key enzymological properties.</span><span style="font-size: 12pt; line-height: 150%;" lang="EN-US"> These਀爀攀猀甀氀琀猀 栀愀瘀攀 椀洀瀀漀爀琀愀渀琀 椀洀瀀氀椀挀愀琀椀漀渀猀 昀漀爀 甀渀搀攀爀猀琀愀渀搀椀渀最 琀栀攀 瀀甀爀椀渀攀 洀攀琀愀戀漀氀椀猀洀 漀昀 
the TB bacillus and </span><span style="font-size: 12pt; line-height: 150%;" lang="EN-US">will serve as an initial step toward the future rational design of਀猀瀀攀挀椀昀椀挀 椀渀栀椀戀椀琀漀爀猀 愀渀搀 搀爀甀最猀 昀漀爀 琀栀椀猀 㰀椀 猀琀礀氀攀㴀∀∀㸀䴀⸀ 
tuberculosis</i> enzyme.</span><span style="font-size: 12pt; line-height: 150%;" lang="EN-US"><o:p></o:p></span></p></font></p><br><b>Palavras-chave: </b>&nbsp;Inosine Monophosphate Dehydrogenase, Mycobacterium tuberculosis, Purine metabolism</td></tr></table></tr></td></table></body></html>਀�