ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:2116-2</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Genética e Biologia Molecular ( Divisão N )</b><p align=justify><strong>YPSB, A NEW DIVISION PROTEIN OF BACILLUS SUBTILIS LIKELY INVOLVED IN SEPTAL PEPTIDOGLYCAN SYNTHESIS.<BR></strong></p><p align=justify><b><u>José Roberto Tavares </u></b> (<i>USP</i>); <b>Robson Francisco Souza </b> (<i>USP</i>); <b>Guilherme Louzada Silva Meira </b> (<i>USP</i>); <b>Frederico José Gueiros Filho </b> (<i>USP</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2><meta http-equiv="Content-Type" content="text/html; charset=utf-8"><meta name="ProgId" content="Word.Document"><meta name="Generator" content="Microsoft Word 11"><meta name="Originator" content="Microsoft Word 11"><link rel="File-List" href="file:///C:%5CDOCUME%7E1%5Cex%5CCONFIG%7E1%5CTemp%5Cmsohtml1%5C01%5Cclip_filelist.xml"><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><style> <!-- /* Font Definitions */ @font-face {font-family:"Book Antiqua"; panose-1:2 4 6 2 5 3 5 3 3 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:647 0 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0in; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:8.5in 11.0in; margin:70.85pt 85.05pt 70.85pt 85.05pt; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> </style><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} </style> <![endif]--><span style="font-size: 12pt; font-family: &quot;Book Antiqua&quot;;" lang="EN-US">The main form of reproduction in prokaryotes is binary division. In <i>Bacillus subtilis </i>this process is executed by the divisome, a macromolecular machine containing at least sixteen proteins which assembles at the site of division. We have used bioinformatics to identify a new division protein of <i style="">B. subtilis</i>, YpsB. Sequence comparison and phylogenetic analysis demonstrated that YpsB is a<span style=""> </span>paralog of the division-site selection protein DivIVA. We used GFP microscopy to determine the subcellular localization of YpsB. This revealed that YpsB is a component of the divisome, and that, similarly to DivIVA, recruitment of YpsB to the divisome requires the late divisome proteins and occurs significantly after Z-ring formation. However, we found that the assembly of YpsB occurs independently of MinCD and DivIVA proteins. Deletion analyses suggest that the N-terminus of YpsB is involved in its targeting to the divisome. YpsB is not essential for septum formation and does not play a role in septum positioning. Nevertheless, a <i>ypsB </i>deletion has a synthetic lethal effect when combined with a deletion of the gene for FtsA<i>,</i> another division protein whose role is to help assemble the FtsZ ring. Fluorescence microscopy of the <i style="">ypsB<sup>-</sup> ftsA<sup>-</sup></i> double-mutant showed filamentation, fragile membrane and cell lyses, suggesting that YpsB is involved in the final steps of division, possibly affecting peptidoglycan synthesis.</span> </font></p><br><b>Palavras-chave: </b>&nbsp;YPSB, DIVISION, Bacillus subtilis, SEPTAL, PEPTIDOGLYCAN</td></tr></table></tr></td></table></body></html>