25º Congresso Brasileiro de Microbiologia
ResumoID:2101-2


Área: Genética e Biologia Molecular ( Divisão N )

BIOCHEMICAL AND KINETIC STUDIES OF POLYNUCLEOTIDE PHOSPHORYLASE (EC 2.7.7.8) FROM MYCOBACTERIUM TUBERCULOSIS

Leonardo Martinelli (CPBMF INCT-TB PUCRS); Luiz Augusto Basso (CPBMF INCT-TB PUCRS); Diógenes Santos (CPBMF INCT-TB PUCRS)

Resumo

Tuberculosis (TB) is the leading cause of death in the world due to infection with a single microbial agent, being mainly caused by Mycobacterium tuberculosis. According to the World Health Organization (WHO), one third of the world population is infected with the tubercle bacillus, nearly 2 billions worldwide, however only 5-10% of the infected people will developed the disease. The co-infection with the human immunodeficiency virus (HIV) increases greatly the chances of the TB reactivation. Regardless of the availability of effective short-course chemotherapy (DOTS), which is preconized by the WHO, and the Bacille Calmette-Guérin (BCG) vaccine, the bacillus continues to claim more lives than any other infectious agent. Due to the outburst of resistant MDR-TB and XDR-TB strains to the classics agents of treatment, a novel line of treatment should be developed with the conception of a new drug and/or vaccine. The enzyme polynucleotide phosphorylase, PNPase (EC 2.7.7.8), encoded by gpsI gene, catalyses the de novo synthesis of polyribonucleotide using nucleoside diphosphates as substrates. Once the role of the PNPase in M. tuberculosis has not been determined yet, it becomes necessary to study this enzyme and its possible role in the bacillus invasion and persistence. The objective of this work is the amplification and cloning of the gene gpsI, the overexpression, purification and characterization of recombinant enzyme to determine its role in M. tuberculosis.

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