<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font  size=1>ResumoID:2058-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Microbiologia Geral ( Divisão H )</b><p align=justify><strong>਀䤀匀伀䰀䄀䴀䔀一吀伀 䔀 䌀䄀刀䄀䌀吀䔀刀䤀娀䄀윀쌀伀 䐀䔀 䴀䄀刀䌀䄀䐀伀刀䔀匀 䴀䤀䌀刀伀匀匀䄀吀준䰀䤀吀䔀匀 倀䄀刀䄀 伀 䄀匀䌀伀䴀䤀䌀䔀吀伀 㰀匀倀䄀一 匀吀夀䰀䔀㴀∀䘀伀一吀ⴀ匀吀夀䰀䔀㨀 䤀吀䄀䰀䤀䌀㬀∀㸀䌀伀䰀䰀䔀吀伀吀刀䤀䌀䠀唀䴀 䄀䌀唀吀䄀吀唀䴀㰀⼀匀倀䄀一㸀Ⰰ 䄀䜀䔀一吀䔀 䐀䄀 倀伀䐀刀䤀䐀쌀伀 䘀䰀伀刀䄀䰀 䐀伀匀 䌀䤀吀刀伀匀     
</strong></p><p align=justify><b><u>Maisa Boff Ciampi </u></b>  (<i>USP - ESALQ</i>); <b>Anete  Pereira de Souza </b>  (<i>IB - UNICAMP</i>); <b>Marcel  Bellato Spósito </b>  (<i>FUNDECITRUS</i>); <b>Lilian  Amorim </b>  (<i>USP - ESALQ</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2><meta http-equiv="Content-Type" content="text/html; charset=utf-8"><meta name="ProgId" content="Word.Document"><meta name="Generator" content="Microsoft Word 11"><meta name="Originator" content="Microsoft Word 11"><link rel="File-List" href="file:///C:%5CUsers%5CMAISAC%7E1%5CAppData%5CLocal%5CTemp%5Cmsohtml1%5C01%5Cclip_filelist.xml"><!--[if gte mso 9]><xml>਀ 㰀眀㨀圀漀爀搀䐀漀挀甀洀攀渀琀㸀 
  <w:View>Normal</w:View>਀  㰀眀㨀娀漀漀洀㸀　㰀⼀眀㨀娀漀漀洀㸀 
  <w:PunctuationKerning/>਀  㰀眀㨀嘀愀氀椀搀愀琀攀䄀最愀椀渀猀琀匀挀栀攀洀愀猀⼀㸀 
  <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid>਀  㰀眀㨀䤀最渀漀爀攀䴀椀砀攀搀䌀漀渀琀攀渀琀㸀昀愀氀猀攀㰀⼀眀㨀䤀最渀漀爀攀䴀椀砀攀搀䌀漀渀琀攀渀琀㸀 
  <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText>਀  㰀眀㨀䌀漀洀瀀愀琀椀戀椀氀椀琀礀㸀 
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   <w:WrapTextWithPunct/>਀   㰀眀㨀唀猀攀䄀猀椀愀渀䈀爀攀愀欀刀甀氀攀猀⼀㸀 
   <w:DontGrowAutofit/>਀  㰀⼀眀㨀䌀漀洀瀀愀琀椀戀椀氀椀琀礀㸀 
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</xml><![endif]--><!--[if gte mso 9]><xml>਀ 㰀眀㨀䰀愀琀攀渀琀匀琀礀氀攀猀 䐀攀昀䰀漀挀欀攀搀匀琀愀琀攀㴀∀昀愀氀猀攀∀ 䰀愀琀攀渀琀匀琀礀氀攀䌀漀甀渀琀㴀∀㄀㔀㘀∀㸀 
 </w:LatentStyles>਀㰀⼀砀洀氀㸀㰀℀嬀攀渀搀椀昀崀ⴀⴀ㸀㰀猀琀礀氀攀㸀 
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	mso-footer-margin:.5in;਀ऀ洀猀漀ⴀ瀀愀瀀攀爀ⴀ猀漀甀爀挀攀㨀　㬀紀 
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	mso-ansi-language:#0400;਀ऀ洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ氀愀渀最甀愀最攀㨀⌀　㐀　　㬀 
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<![endif]--><span style="font-size: 10pt; font-family: Arial;" lang="PT-BR">A migração da citricultura para਀愀 爀攀最椀漀 猀甀搀漀攀猀琀攀 搀漀 䔀猀琀愀搀漀 搀攀 匀漀 倀愀甀氀漀 琀攀洀 猀椀搀漀 昀愀瘀漀爀瘀攀氀  漀挀漀爀爀渀挀椀愀 搀愀 
podridão floral dos citros, causada pelo ascomiceto <span style="font-style: italic;">Colletotrichum acutatum</span>, acarretando a abscisão de frutos jovens.਀䄀瀀攀猀愀爀 搀愀 椀洀瀀漀爀琀渀挀椀愀 搀漀 瀀愀琀最攀渀漀 瀀愀爀愀 愀 挀甀氀琀甀爀愀 搀攀 挀椀琀爀漀猀Ⰰ 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀　瀀琀㬀 昀漀渀琀ⴀ昀愀洀椀氀礀㨀 䄀爀椀愀氀㬀∀ 氀愀渀最㴀∀倀吀ⴀ䈀刀∀㸀渀漀 栀 椀渀昀漀爀洀愀攀猀 猀漀戀爀攀 猀甀愀 攀猀琀爀甀琀甀爀愀 最攀渀琀椀挀漀ⴀ瀀漀瀀甀氀愀挀椀漀渀愀氀 渀漀 䔀猀琀愀搀漀⸀ 
Para determinar a extensão da diferenciação genética dentro e entre as਀瀀漀瀀甀氀愀攀猀 搀攀 㰀⼀猀瀀愀渀㸀㰀椀 猀琀礀氀攀㴀∀∀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀　瀀琀㬀 昀漀渀琀ⴀ昀愀洀椀氀礀㨀 䄀爀椀愀氀㬀∀ 氀愀渀最㴀∀倀吀ⴀ䈀刀∀㸀䌀⸀ 
acutatum</span></i><span style="font-size: 10pt; font-family: Arial;" lang="PT-BR"> infectando citros, detectar਀洀椀最爀愀渀琀攀猀 攀 愀猀 瀀爀漀瘀瘀攀椀猀 爀漀琀愀猀 洀椀最爀愀琀爀椀愀猀 搀漀 瀀愀琀最攀渀漀Ⰰ 琀漀爀渀愀ⴀ猀攀 
fundamental o desenvolvimento de marcadores moleculares adequados, que permitam਀搀椀昀攀爀攀渀挀椀愀爀 最攀渀琀椀瀀漀猀 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀　瀀琀㬀 昀漀渀琀ⴀ昀愀洀椀氀礀㨀 䄀爀椀愀氀㬀∀ 氀愀渀最㴀∀倀吀ⴀ䈀刀∀㸀搀漀 昀甀渀最漀㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀　瀀琀㬀 昀漀渀琀ⴀ昀愀洀椀氀礀㨀 䄀爀椀愀氀㬀∀ 氀愀渀最㴀∀倀吀ⴀ䈀刀∀㸀 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀　瀀琀㬀 昀漀渀琀ⴀ昀愀洀椀氀礀㨀 䄀爀椀愀氀㬀∀ 氀愀渀最㴀∀倀吀ⴀ䈀刀∀㸀渀愀猀 搀椀昀攀爀攀渀琀攀猀 瀀漀瀀甀氀愀攀猀 愀洀漀猀琀爀愀搀愀猀⸀ 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀　瀀琀㬀 昀漀渀琀ⴀ昀愀洀椀氀礀㨀 䄀爀椀愀氀㬀∀ 氀愀渀最㴀∀倀吀ⴀ䈀刀∀㸀一攀猀琀攀 攀猀琀甀搀漀 昀漀爀愀洀 搀攀猀攀渀瘀漀氀瘀椀搀漀猀 ㈀㜀 洀愀爀挀愀搀漀爀攀猀 
microssatélites para <i>C. acutatum</i>, a partir da construção de um banco genômico਀攀渀爀椀焀甀攀挀椀搀漀 搀攀 洀椀挀爀漀猀猀愀琀氀椀琀攀猀⸀ 䘀漀爀愀洀 攀渀挀漀渀琀爀愀搀漀猀 ㈀㜀 洀椀挀爀漀猀猀愀琀氀椀琀攀猀Ⰰ 猀攀渀搀漀 琀爀猀 挀漀洀瀀漀猀琀漀猀 攀 ㈀㐀 猀椀洀瀀氀攀猀Ⰰ 搀攀渀琀爀攀 漀猀 焀甀愀椀猀 ㈀㄀ 搀椀渀甀挀氀攀漀琀搀攀漀猀Ⰰ 甀洀 
trinucleotídeo e dois pentanucleotídeos. Dos 27 pares de primers testados em 36਀椀猀漀氀愀搀漀猀 洀漀渀漀猀瀀爀椀挀漀猀 搀漀 瀀愀琀最攀渀漀Ⰰ 愀洀漀猀琀爀愀搀漀猀 搀攀 猀攀椀猀 瀀漀瀀甀氀愀攀猀 搀椀猀琀椀渀琀愀猀 攀洀 
São Paulo, 24 (89%) amplificaram os locos microssatélites corretamente por PCR,਀挀漀洀 琀攀洀瀀攀爀愀琀甀爀愀猀 搀攀 愀渀攀氀愀洀攀渀琀漀 瘀愀爀椀愀渀搀漀 搀攀 㐀㠀뀀䌀 愀 㘀㈀뀀䌀⸀ 䐀漀猀 ㈀㐀 氀漀挀漀猀 愀洀瀀氀椀昀椀挀愀搀漀猀Ⰰ 搀攀稀 ⠀㐀㈀─⤀ 昀漀爀愀洀 瀀漀氀椀洀爀昀椀挀漀猀 渀愀 愀洀漀猀琀爀愀 琀攀猀琀愀搀愀Ⰰ 愀瀀爀攀猀攀渀琀愀渀搀漀 
de 2 a 4 alelos, com média de 2,2 alelos por loco, sendo que </span><span style="font-size: 10pt; font-family: Arial;" lang="PT-BR">14 locos não਀攀砀椀戀椀爀愀洀 瘀愀爀椀愀漀 愀氀氀椀挀愀㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀　瀀琀㬀 昀漀渀琀ⴀ昀愀洀椀氀礀㨀 䄀爀椀愀氀㬀∀ 氀愀渀最㴀∀倀吀ⴀ䈀刀∀㸀⸀ 䔀猀猀攀猀 洀愀爀挀愀搀漀爀攀猀 猀攀爀漀 
extremamente úteis para os estudos de genética de populações do patógeno que਀攀猀琀漀 猀攀渀搀漀 挀漀渀搀甀稀椀搀漀猀⸀ 䤀渀瘀攀猀琀椀最愀渀搀漀 愀 攀猀琀爀甀琀甀爀愀 瀀漀瀀甀氀愀挀椀漀渀愀氀 搀攀 瀀愀琀最攀渀漀猀Ⰰ  瀀漀猀猀瘀攀氀 椀渀昀攀爀椀爀 愀 搀椀猀琀渀挀椀愀 攀昀攀琀椀瘀愀 搀攀 搀椀猀瀀攀爀猀漀 搀攀 瀀爀漀瀀最甀氀漀猀Ⰰ 愀猀 昀漀渀琀攀猀 
potenciais de diversidade genética e se a principal estratégia਀爀攀瀀爀漀搀甀琀椀瘀愀  猀攀砀甀愀氀 漀甀 愀猀猀攀砀甀愀氀⸀ 䄀 搀椀昀攀爀攀渀挀椀愀漀 洀漀氀攀挀甀氀愀爀 搀攀 瀀漀瀀甀氀愀攀猀 搀攀 㰀椀㸀䌀⸀ 
acutatum</i> facilitará também estudos epidemiológicos, além de auxiliar os਀洀攀氀栀漀爀椀猀琀愀猀 搀愀 挀甀氀琀甀爀愀 愀 愀瀀攀爀昀攀椀漀愀爀 愀猀 攀猀琀爀愀琀最椀愀猀 瀀愀爀愀 猀攀氀攀漀 搀攀 瘀愀爀椀攀搀愀搀攀猀 
resistentes ao patógeno.</span>਀ 
</font></p><br><b>Palavras-chave: </b>&nbsp;estrutura populacional, epidemiologia molecular, fitopatologia, marcadores moleculares, genotipagem</td></tr></table></tr></td></table></body></html>਀�