| Área: Microbiologia Veterinária ( Divisão G ) PRODUÇÃO E CARACTERIZAÇÃO DE UMA PROTEÍNA SECRETADA EXCLUSIVA DE CORYNEBACTERIUM PSEUDOTUBERCULOSIS PARA O DIAGNÓSTICO DA LINFADENITE CASEOSA
Leonardo Mendes Castro Alves (UFMG); Núbia Seyffert (UFMG); Tessália Diniz Luerce Saraiva (UFMG); Priscila Grynberg (UFMG); Wanderson Marques da Silva (UFMG); Luis Gustavo Carvalho Pacheco (UFMG); Fernanda Alves Dorella (UFMG); Roberto José Meyer Nascimento (UFBA); Anderson Miyoshi (UFMG); Vasco Azevedo (UFMG)
Resumo䰀椀渀昀愀搀攀渀椀琀攀 挀愀猀攀漀猀愀 ⠀䰀䌀⤀ 甀洀愀 搀漀攀渀愀 椀渀昀攀挀琀漀ⴀ挀漀渀琀愀最椀漀猀愀 挀爀渀椀挀愀 焀甀攀 愀挀漀洀攀琀攀 瀀攀焀甀攀渀漀猀 爀甀洀椀渀愀渀琀攀猀⸀ 䄀 攀渀昀攀爀洀椀搀愀搀攀 挀愀爀愀挀琀攀爀椀稀愀搀愀 瀀攀氀愀 昀漀爀洀愀漀 搀攀 愀戀猀挀攀猀猀漀猀 猀甀瀀攀爀昀椀挀椀愀椀猀 漀甀 瘀椀猀挀攀爀愀椀猀Ⰰ 攀 攀猀琀 愀猀猀漀挀椀愀搀愀 愀 最爀愀渀搀攀猀 瀀爀攀樀甀稀漀猀 渀愀 漀瘀椀渀漀挀愀瀀爀椀渀漀挀甀氀琀甀爀愀Ⰰ 瀀攀氀愀 瀀攀爀搀愀 搀攀 瀀攀猀漀Ⰰ 搀攀昀椀挀椀渀挀椀愀 爀攀瀀爀漀搀甀琀椀瘀愀 攀 爀攀搀甀漀 渀愀 瀀爀漀搀甀漀 搀攀 氀 搀漀猀 愀渀椀洀愀椀猀 椀渀昀攀挀琀愀搀漀猀⸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀琀礀氀攀㨀 椀琀愀氀椀挀㬀∀㸀 䌀漀爀礀渀攀戀愀挀琀攀爀椀甀洀 瀀猀攀甀搀漀琀甀戀攀爀挀甀氀漀猀椀猀㰀⼀猀瀀愀渀㸀 ⠀䌀倀⤀Ⰰ 猀攀甀 愀最攀渀琀攀 攀琀椀漀氀最椀挀漀Ⰰ 琀洀 猀椀搀漀 愀氀瘀漀 搀攀 椀渀琀攀渀猀漀猀 攀猀琀甀搀漀猀 瀀愀爀愀 漀 搀攀猀攀渀瘀漀氀瘀椀洀攀渀琀漀 搀攀 甀洀 洀琀漀搀漀 搀椀愀最渀猀琀椀挀漀 爀瀀椀搀漀Ⰰ 瀀爀攀挀椀猀漀 攀 挀漀洀 戀愀椀砀漀 挀甀猀琀漀⸀ 一攀猀琀攀 琀爀愀戀愀氀栀漀 搀攀猀挀爀攀瘀攀洀漀猀 愀 瀀爀漀搀甀漀 栀攀琀攀爀氀漀最愀 搀愀 瀀爀漀琀攀渀愀 搀愀 䌀瀀猀攀挀㌀㤀 攀洀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀琀礀氀攀㨀 椀琀愀氀椀挀㬀∀㸀䔀猀挀栀攀爀椀挀栀椀愀 挀漀氀椀㰀⼀猀瀀愀渀㸀⸀ 䄀 䌀瀀猀攀挀㌀㤀 昀漀椀 瀀爀攀搀椀琀愀 挀漀洀漀 攀砀瀀漀爀琀愀搀愀 攀Ⰰ 挀漀洀漀 攀砀挀氀甀猀椀瘀愀 搀攀 䌀倀Ⰰ 瀀漀搀攀 爀攀瀀爀攀猀攀渀琀愀爀 甀洀 愀氀瘀漀 椀渀琀攀爀攀猀猀愀渀琀攀 瀀愀爀愀 漀 搀攀猀攀渀瘀漀氀瘀椀洀攀渀琀漀 搀攀 甀洀 琀攀猀琀攀 搀椀愀最渀猀琀椀挀漀 愀挀甀爀愀搀漀⸀ 倀愀爀愀 愀 挀愀爀愀挀琀攀爀椀稀愀漀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀琀礀氀攀㨀 椀琀愀氀椀挀㬀∀㸀椀渀 猀椀氀椀挀漀㰀⼀猀瀀愀渀㸀 昀漀爀愀洀 甀琀椀氀椀稀愀搀漀猀 漀猀 瀀爀漀最爀愀洀愀猀㨀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀琀礀氀攀㨀 椀琀愀氀椀挀㬀∀㸀䜀氀椀洀洀攀爀㰀⼀猀瀀愀渀㸀Ⰰ 瀀愀爀愀 搀攀琀攀爀洀椀渀愀漀 搀愀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀琀礀氀攀㨀 椀琀愀氀椀挀㬀∀㸀伀刀䘀㰀⼀猀瀀愀渀㸀㬀 戀氀愀猀琀渀Ⰰ 戀氀愀猀琀砀 攀 戀氀愀猀琀瀀 挀漀渀琀爀愀 漀 戀愀渀挀漀 搀攀 搀愀搀漀猀 渀漀ⴀ爀攀搀甀渀琀愀渀琀攀猀 搀漀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀琀礀氀攀㨀 椀琀愀氀椀挀㬀∀㸀䜀攀渀䈀愀渀欀 㰀⼀猀瀀愀渀㸀瀀愀爀愀 愀 爀攀愀氀椀稀愀漀 搀愀猀 愀渀氀椀猀攀猀 搀攀 猀椀洀椀氀愀爀椀搀愀搀攀猀㬀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀琀礀氀攀㨀 椀琀愀氀椀挀㬀∀㸀䤀渀琀攀爀倀爀漀匀挀愀渀㰀⼀猀瀀愀渀㸀Ⰰ 瀀愀爀愀 愀猀 戀甀猀挀愀猀 瀀漀爀 搀漀洀渀椀漀猀 挀漀渀猀攀爀瘀愀搀漀猀 攀 漀猀 瀀爀漀最爀愀洀愀猀 吀䴀䠀䴀䴀 ㈀⸀ 攀 匀椀最渀愀氀倀 ㌀⸀ ☀渀戀猀瀀㬀 瀀愀爀愀 椀搀攀渀琀椀昀椀挀愀漀 搀漀 瀀攀瀀琀搀攀漀 猀椀渀愀氀 攀 搀漀洀渀椀漀 琀爀愀渀猀洀攀洀戀爀渀椀挀漀⸀ 倀愀爀愀 愀 挀氀漀渀愀最攀洀 搀愀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀琀礀氀攀㨀 椀琀愀氀椀挀㬀∀㸀挀瀀猀攀挀㌀㤀㰀⼀猀瀀愀渀㸀 甀琀椀氀椀稀漀甀ⴀ猀攀 漀 瘀攀琀漀爀 瀀匀䴀䄀刀吀 瀀攀爀琀攀挀攀渀琀攀 愀漀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀琀礀氀攀㨀 椀琀愀氀椀挀㬀∀㸀䬀椀琀☀渀戀猀瀀㬀 匀漀昀琀愀最∀‡倀爀漀琀攀椀渀 䔀砀瀀爀攀猀猀椀漀渀 ☀愀洀瀀㬀 倀甀爀椀昀椀挀愀琀椀漀渀 匀礀猀琀攀洀㰀⼀猀瀀愀渀㸀⸀ 伀 渀瘀攀氀 搀攀 攀砀瀀爀攀猀猀漀 昀漀椀 愀瘀愀氀椀愀搀漀 攀洀 焀甀愀琀爀漀 搀椀昀攀爀攀渀琀攀猀 氀椀渀栀愀最攀渀猀 伀瘀攀爀䔀砀瀀爀攀猀猀∀‡⠀䌀㐀⼀䐀䔀㌀ 瀀䰀礀猀匀Ⰰ 䌀㐀⼀䐀䔀㌀Ⰰ 䌀㐀㌀⼀䐀䔀㌀Ⰰ 䌀㐀㌀⼀䐀䔀㌀ 瀀䰀礀猀匀⤀Ⰰ 搀攀渀琀爀攀 愀猀 焀甀愀椀猀 昀漀椀 漀戀猀攀爀瘀愀搀愀 甀洀愀 洀愀椀漀爀 瀀爀漀搀甀漀 搀愀 瀀爀漀琀攀渀愀 渀愀 伀瘀攀爀䔀砀瀀爀攀猀猀∀‡䌀㐀⼀䐀䔀㌀ 瀀䰀礀猀匀⸀ 䄀 瀀甀爀椀昀椀挀愀漀 搀愀 爀䌀瀀猀攀挀㌀㤀 昀漀椀 爀攀愀氀椀稀愀搀愀 瀀漀爀 攀氀攀琀爀漀攀氀甀椀漀⸀ 䄀 爀䌀瀀猀攀挀㌀㤀 攀猀琀 猀攀渀搀漀 猀甀戀洀攀琀椀搀愀 愀 琀攀猀琀攀猀 椀洀甀渀漀氀最椀挀漀猀 攀 洀漀氀攀挀甀氀愀爀攀猀 瀀愀爀愀 愀 瀀漀猀琀攀爀椀漀爀 猀攀氀攀漀 搀漀 洀琀漀搀漀 搀椀愀最渀猀琀椀挀漀 愀 猀攀爀 攀洀瀀爀攀最愀搀漀 瀀愀爀愀 愀 攀氀愀戀漀爀愀漀 搀攀 甀洀 攀渀猀愀椀漀 猀攀渀猀瘀攀氀 攀 攀猀瀀攀挀昀椀挀漀 瀀愀爀愀 愀 搀攀琀攀挀漀 搀愀 䰀䌀⸀ 䄀琀 漀 洀漀洀攀渀琀漀Ⰰ 漀猀 爀攀猀甀氀琀愀搀漀猀 搀攀洀漀渀猀琀爀愀洀 愀 椀洀瀀漀爀琀渀挀椀愀 搀攀猀猀愀 瀀爀漀琀攀渀愀 渀愀 攀氀愀戀漀爀愀漀 搀攀 甀洀 琀攀猀琀攀 搀椀愀最渀猀琀椀挀漀 瀀愀爀愀 䰀䌀 攀洀 瀀攀焀甀攀渀漀猀 爀甀洀椀渀愀渀琀攀猀Ⰰ 焀甀攀 瀀漀搀攀爀 挀漀渀琀爀椀戀甀椀爀 瀀愀爀愀 漀 挀漀渀琀爀漀氀攀 搀愀 搀漀攀渀愀 渀愀 挀愀瀀爀椀渀漀瘀椀渀漀挀甀氀琀甀爀愀 渀愀挀椀漀渀愀氀⸀㰀戀爀㸀㰀戀爀㸀㰀洀攀琀愀 栀琀琀瀀ⴀ攀焀甀椀瘀㴀∀䌀漀渀琀攀渀琀ⴀ吀礀瀀攀∀ 挀漀渀琀攀渀琀㴀∀琀攀砀琀⼀栀琀洀氀㬀 挀栀愀爀猀攀琀㴀甀琀昀ⴀ㠀∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀倀爀漀最䤀搀∀ 挀漀渀琀攀渀琀㴀∀圀漀爀搀⸀䐀漀挀甀洀攀渀琀∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䜀攀渀攀爀愀琀漀爀∀ 挀漀渀琀攀渀琀㴀∀䴀椀挀爀漀猀漀昀琀 圀漀爀搀 ∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀伀爀椀最椀渀愀琀漀爀∀ 挀漀渀琀攀渀琀㴀∀䴀椀挀爀漀猀漀昀琀 圀漀爀搀 ∀㸀㰀氀椀渀欀 爀攀氀㴀∀䘀椀氀攀ⴀ䰀椀猀琀∀ 栀爀攀昀㴀∀昀椀氀攀㨀⼀⼀⼀䌀㨀─㔀䌀䐀伀䌀唀䴀䔀─㜀䔀─㔀䌀䄀氀甀渀漀猀开㈀─㔀䌀䌀伀一䘀䤀䜀─㜀䔀─㔀䌀吀攀洀瀀─㔀䌀洀猀漀栀琀洀氀─㔀䌀 ─㔀䌀挀氀椀瀀开昀椀氀攀氀椀猀琀⸀砀洀氀∀㸀㰀℀ⴀⴀ嬀椀昀 最琀攀 洀猀漀 㤀崀㸀㰀砀洀氀㸀
㰀眀㨀嘀椀攀眀㸀一漀爀洀愀氀㰀⼀眀㨀嘀椀攀眀㸀
0 㰀眀㨀䠀礀瀀栀攀渀愀琀椀漀渀娀漀渀攀㸀㈀㰀⼀眀㨀䠀礀瀀栀攀渀愀琀椀漀渀娀漀渀攀㸀
㰀眀㨀嘀愀氀椀搀愀琀攀䄀最愀椀渀猀琀匀挀栀攀洀愀猀⼀㸀
false 㰀眀㨀䤀最渀漀爀攀䴀椀砀攀搀䌀漀渀琀攀渀琀㸀昀愀氀猀攀㰀⼀眀㨀䤀最渀漀爀攀䴀椀砀攀搀䌀漀渀琀攀渀琀㸀
false 㰀眀㨀䌀漀洀瀀愀琀椀戀椀氀椀琀礀㸀
㰀眀㨀匀渀愀瀀吀漀䜀爀椀搀䤀渀䌀攀氀氀⼀㸀
㰀眀㨀唀猀攀䄀猀椀愀渀䈀爀攀愀欀刀甀氀攀猀⼀㸀
㰀⼀眀㨀䌀漀洀瀀愀琀椀戀椀氀椀琀礀㸀
MicrosoftInternetExplorer4 㰀⼀眀㨀圀漀爀搀䐀漀挀甀洀攀渀琀㸀
㰀⼀猀琀礀氀攀㸀㰀℀ⴀⴀ嬀椀昀 最琀攀 洀猀漀 崀㸀
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