25º Congresso Brasileiro de Microbiologia
ResumoID:2014-1


Área: Microbiologia Clinica ( Divisão A )

COMPARISON BETWEEN CONVENTIONAL, IMMUNOLOGICAL AND MOLECULAR DIAGNOSIS OF CAMPYLOBACTER SP. IN CHILDREN WITH DIARRHEA ATTENDED AT OUTPATIENT CLINICS IN NORTHEASTERN BRAZIL

Josiane da Silva Quetz (UFC); Ila Fernanda Nunes Lima (UFC); Renata de Sousa Alves (UFC); Alexandre Havt (UFC); Alberto Melo Soares (UFC); Luís Carlos Rey (UFC); Bernhard Weigl (PATH); Aldo Ângelo Moreira Lima (UFC)

Resumo

INTRODUCTION: Campylobacter enteritis is a common cause of acute bacterial diarrhea worldwide. Polymerase chain rection (PCR) have been increasingly applied to diagnose several pathogenic bacteria. Our aim was to compare microbiological conventional diagnosis, PCR detection, and immunological identification of Campylobacter sp. directly from fecal samples.

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RESULTS: Of the 24 samples tested, 23 (95.8%) were positive for one or both methods, where 19 (79.2%) were positive by PCR and 23 (95.8%) by ELISA. All 19 positive samples by PCR were identified as C. jejuni (hipO+) and none of them was C. coli (ask-). The negative samples for any PCR were submitted to a new extraction and PCR detection. No differences were observed between both data. The CDT genes were found in all hipO+ samples.   

਀㰀倀 挀氀愀猀猀㴀眀攀猀琀攀爀渀 猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀  挀洀  挀洀  瀀琀㬀 䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀 ㄀㄀㔀─㬀 吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀 樀甀猀琀椀昀礀∀㸀㰀䈀㸀㰀匀倀䄀一 氀愀渀最㴀䔀一ⴀ唀匀 猀琀礀氀攀㴀∀䘀伀一吀ⴀ匀䤀娀䔀㨀 ㄀ 瀀琀㬀 䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀 ㄀㄀㔀─㬀 䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀䄀爀椀愀氀✀Ⰰ✀猀愀渀猀ⴀ猀攀爀椀昀✀㬀 洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀䌀伀一䌀䰀唀匀䤀伀一㨀㰀⼀匀倀䄀一㸀㰀⼀䈀㸀㰀匀倀䄀一 氀愀渀最㴀䔀一ⴀ唀匀 猀琀礀氀攀㴀∀䘀伀一吀ⴀ匀䤀娀䔀㨀 ㄀ 瀀琀㬀 䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀 ㄀㄀㔀─㬀 䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀䄀爀椀愀氀✀Ⰰ✀猀愀渀猀ⴀ猀攀爀椀昀✀㬀 洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀 吀栀攀 爀攀猀甀氀琀猀 猀栀漀眀攀搀 琀栀愀琀 倀䌀刀 愀渀搀 椀洀洀甀渀漀氀漀最椀挀愀氀 椀搀攀渀琀椀昀椀挀愀琀椀漀渀猀 挀愀渀 搀攀琀攀挀琀 㰀䤀㸀䌀愀洀瀀礀氀漀戀愀挀琀攀爀 㰀⼀䤀㸀猀瀀⸀ 搀椀爀攀挀琀氀礀 昀爀漀洀 昀攀挀愀氀 猀愀洀瀀氀攀猀 愀猀 最漀漀搀 愀猀 洀椀挀爀漀戀椀漀氀漀最椀挀愀氀 挀漀渀瘀攀渀琀椀漀渀愀氀 搀椀愀最渀漀猀椀猀⸀ 䠀漀眀攀瘀攀爀Ⰰ 琀栀攀 昀椀爀猀琀 琀眀漀 搀椀愀最渀漀猀琀椀挀 洀攀琀栀漀搀猀 挀愀渀 最椀瘀攀 爀攀猀甀氀琀猀 漀渀 琀栀攀 猀愀洀攀 搀愀礀⸀ 䌀漀渀猀椀搀攀爀椀渀最 猀琀漀漀氀 挀甀氀琀甀爀攀 愀猀 最漀氀搀 猀琀愀渀搀愀爀搀 洀攀琀栀漀搀Ⰰ 䔀䰀䤀匀䄀 爀攀猀甀氀琀猀 眀攀爀攀 洀漀爀攀 猀椀洀椀氀愀爀 琀栀愀渀 倀䌀刀 搀攀琀攀挀琀椀漀渀⸀ 吀栀攀 漀挀挀甀爀爀攀渀挀攀 漀昀 椀洀洀甀渀漀氀漀最椀挀愀氀 挀爀漀猀猀ⴀ爀攀愀挀琀椀漀渀 眀椀琀栀 漀琀栀攀爀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䌀愀洀瀀礀氀漀戀愀挀琀攀爀㰀⼀䤀㸀 猀瀀攀挀椀攀猀 洀甀猀琀 戀攀 挀漀渀猀椀搀攀爀攀搀⸀ 䤀渀 愀搀搀椀琀椀漀渀Ⰰ 䐀一䄀 攀砀琀爀愀挀琀椀漀渀 瀀爀漀挀攀猀猀 挀漀甀氀搀 戀攀 椀渀瘀漀氀瘀攀搀 椀渀 倀䌀刀 爀攀猀甀氀琀猀⸀㰀匀倀䄀一 猀琀礀氀攀㴀∀洀猀漀ⴀ猀瀀愀挀攀爀甀渀㨀 礀攀猀∀㸀☀渀戀猀瀀㬀☀渀戀猀瀀㬀☀渀戀猀瀀㬀☀渀戀猀瀀㬀 㰀⼀匀倀䄀一㸀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀

FINNANCIAL SUPPORT: CNPq (Brazil) and PATH (USA)

਀㰀倀 挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀 猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀  挀洀  挀洀  瀀琀∀㸀㰀匀倀䄀一 氀愀渀最㴀䔀一ⴀ唀匀㸀㰀漀㨀瀀㸀㰀䘀伀一吀 昀愀挀攀㴀䌀愀氀椀戀爀椀㸀☀渀戀猀瀀㬀㰀⼀䘀伀一吀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀倀愀氀愀瘀爀愀猀ⴀ挀栀愀瘀攀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀䌀愀洀瀀礀氀漀戀愀挀琀攀爀 猀瀀⸀Ⰰ 䌀䠀䤀䰀䐀䠀伀伀䐀 䐀䤀䄀刀刀䠀䔀䄀Ⰰ 䐀䤀䄀䜀一伀匀吀䤀䌀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀