ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:1952-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Microbiologia Geral e Meio Ambiente ( Divisão L )</b><p align=justify><strong><FONT SIZE="2">OIL DEGRADING BACTERIAL COMMUNITIES ASSOCIATED WITH GUANABARA BAY MANGROVES AND THEIR POTENTIAL FOR PHYTOREMEDIATION</FONT> </strong></p><p align=justify><b>Newton Carlos Maçal Gomes </b> (<i>UA</i>); <b>Simone Dealtry </b> (<i>UFRJ</i>); <b><u>Angela Michelato Ghizelini </u></b> (<i>UFRJ</i>); <b>Cecilia Flocco </b> (<i>JKI</i>); <b>Leda Cristina Mendonça-hagler </b> (<i>UFRJ</i>); <b>Kornelia Smalla </b> (<i>JKI</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2> <div style="text-align: justify;"><p><link rel="File-List" href="file:///C:%5CDOCUME%7E1%5CUsuario%5CCONFIG%7E1%5CTemp%5Cmsohtml1%5C01%5Cclip_filelist.xml"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="stockticker"></o:smarttagtype><font size="1"><span style="font-size: 12pt; font-family: Arial,Helvetica,sans-serif;" lang="EN-GB">Mangroves are ecologically important intertidal estuarine wetlands which are disappearing worldwide. Oil spills, transport activities and industrial discharge are among the typical sources of hydrocarbon pollution in urban mangroves. In this study, an attempt was made to enrich petroleum hydrocarbon-degrading cultures (PHDC) from bulk and rhizosphere sediments of a mangrove chronically polluted with oil hydrocarbons. The evaluation of mixed cultures showed higher levels of petroleum hydrocarbon (PH) removal in systems containing mineral media (</span><st1:stockticker style="font-family: Arial,Helvetica,sans-serif;"><span style="font-size: 12pt;" lang="EN-GB">MSM</span></st1:stockticker><span style="font-size: 12pt; font-family: Arial,Helvetica,sans-serif;" lang="EN-GB">) amended with petroleum as sole carbon source and inoculated with rhizosphere than in sediment PHDC. After 5 days of incubation, the final concentration of PH in flasks containing sediment PHDC was moderately lower than in the control. Flasks inoculated with rhizosphere PHDC showed a sharp decrease in PH concentration. The variance analyses indicated significant differences of PH removal among enriched cultures from bulk and rhizosphere sediment samples (P&lt;0.5). Different <i>ndo</i> genes and plasmids groups were enhanced from sediment and rhizosphere samples after culture enrichment in </span><st1:stockticker style="font-family: Arial,Helvetica,sans-serif;"><span style="font-size: 12pt;" lang="EN-GB">MSM</span></st1:stockticker><span style="font-size: 12pt; font-family: Arial,Helvetica,sans-serif;" lang="EN-GB"> media. PCR-SB hybridization analysis showed that the <span style="font-style: italic;">ndo</span> genotypes <i>nag</i>, <i>phn</i> and <i>nah</i> and broad range plasmids belonging to incP-1 and incP-9 were enhanced in nearly all enriched cultures. <i style="">Bacteria</i> and taxon specific DGGE analyses were used to investigate the structural diversity of petroleum enriched cultures derived from rhizosphere and sediment samples. While <i>Bacteria</i> and <i>Alphaproteobacteria</i> DGGE fingerprint analyses revealed complex profiles, a rather low complexity of DGGE-ribotypes was detected for <i>Betaproteobacteria</i>, <i>Actinobacteria</i> and <i>Pseudomonas</i>. The rhizosphere PHDC formed mainly two clusters, which were better resolved by the second principal component analysis, detecting differences between PHDC. Some dominant ribotypes were common for all rhizosphere and sediment PHDC. The combination of conventional batch enrichment cultures and molecular analyses indicated that this environment has a latent potential to degrade petroleum hydrocarbons. Our results are relevant to help develop a phytoremediation strategy to mitigate the effect of oil pollution on urban mangrove forests and provided new insights on this complex environment, unravelling mechanisms and players in the process of hydrocarbon degradation.</span></font></p><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>21</w:HyphenationZone> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:ApplyBreakingRules/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> </w:Compatibility> </w:WordDocument> </xml><![endif]--><!--[if !mso]><object classid="clsid:38481807-CA0E-42D2-BF39-B33AF135CC4D" id=ieooui></object> <style> st1:*{behavior:url(#ieooui) } </style> <![endif]--><style> <!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:DE; mso-fareast-language:PT;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> </style><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:Calibri; mso-fareast-font-family:Calibri;} </style> <![endif]--><p style="font-family: Arial,Helvetica,sans-serif;"><font size="1"><span style="font-size: 12pt;" lang="EN-GB">Apoio: DFG-DE, FAPERJ, CAPES, PBV.</span></font></p><span style="font-size: 12pt; font-family: &quot;Times New Roman&quot;;" lang="EN-GB"></span></div> </font></p><br><b>Palavras-chave: </b>&nbsp;Bacterial Communities, DGGE, Mangroves, Oil degradation, Phytoremediation</td></tr></table></tr></td></table></body></html>