ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:1803-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Microbiologia Clinica ( Divisão A )</b><p align=justify><strong>QUINOLONE-RESISTANT ISOLATES OF ENTEROBACTERIACEAE FROM COMMUNITY-ACQUIRED URINARY TRACT INFECTIONS</strong></p><p align=justify><b>Maria Carolina Albuquerque Wanderley </b> (<i>UPE</i>); <b>Thammy Moura Samico </b> (<i>UPE</i>); <b>Anna Carolina Soares Almeida </b> (<i>UPE</i>); <b>Felipe Lira de Sá Cavalcanti </b> (<i>UPE</i>); <b>Marinalda Anselmo Vilela </b> (<i>UPE</i>); <b><u>Marcia Maria Camargo de Morais </u></b> (<i>UPE</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2><P align=justify><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Arial; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-bidi-language: AR-SA">Fluoroquinolones have been used for treatment of urinary tract infections (UTI) successfully. These antimicrobials inhibit the enzyme DNA gyrase in gram negative bacteria and Topoisomerase IV in gram positive bacteria. </SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Arial; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-bidi-language: AR-SA">Resistance to fluoroquinolones occurs mainly as a result of mutations in bacterial <I>gyr</I>A and <I>par</I>C genes that code for DNA gyrase and topoisomerase IV, respectively.</SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Arial; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-bidi-language: AR-SA"> However, it was recently described a new resistance mechanism, which is provided by <I style="mso-bidi-font-style: normal">qnr</I> gene. The aim this work was to identify quinolone-resistant urine isolates in Enterobacteriaceae from community, to characterize their susceptibility profiles and to investigate the presence of ESBL-mediated </SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Symbol; mso-fareast-language: EN-US; mso-bidi-font-family: Arial; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-bidi-language: AR-SA; mso-ascii-font-family: Arial; mso-hansi-font-family: Arial; mso-char-type: symbol; mso-symbol-font-family: Symbol"><SPAN style="mso-char-type: symbol; mso-symbol-font-family: Symbol"><SPAN style="mso-spacerun: yes">&nbsp;</SPAN></SPAN></SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Symbol; mso-fareast-language: EN-US; mso-bidi-font-family: Arial; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-bidi-language: AR-SA; mso-ascii-font-family: Arial; mso-hansi-font-family: Arial; mso-char-type: symbol; mso-symbol-font-family: Symbol"><SPAN style="mso-char-type: symbol; mso-symbol-font-family: Symbol">b</SPAN></SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Arial; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-bidi-language: AR-SA">-lactam resistance. A total of 24 samples </SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Arial; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-bidi-language: AR-SA">were obtained from community-acquired UTIs.</SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Arial; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-bidi-language: AR-SA"> Results showed that the isolated bacteria were <I style="mso-bidi-font-style: normal">Escherichia coli</I> (83%) and <I style="mso-bidi-font-style: normal">Klebsiella </I>spp. (17%). Antimicrobial activities of ciprofloxacin (CIP), cefotaxime (CTX) and sultamethazole-trimethoprim (SUT) were determined by disk diffusion method, according to the Clinical Laboratory Standards Institute (CLSI) guidelines. All the samples </SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Arial; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-bidi-language: AR-SA">were observed as resistant to ciprofloxacin.</SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Arial; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-bidi-language: AR-SA"> Resistance to SUT was 75%, while resistance to CTX and GEN were 25% and 38%, respectively. The quinolone resistance was also accompanied by ESBL production in 17% of the isolates. When taken separately, only 5% of <I style="mso-bidi-font-style: normal">E. Coli</I> isolates showed ESBL production, in contrast to 60% of <I style="mso-bidi-font-style: normal">Klebsiella</I> spp. isolates. </SPAN><SPAN lang=EN-US style="FONT-SIZE: 10pt; LINE-HEIGHT: 115%; FONT-FAMILY: Arial; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-bidi-language: AR-SA">These findings support the suggestion that antibiotic resistance mechanisms are spreading more easily in bacteria causing community-acquired UTIs. The detection of Enterobacteriaceae´s samples with quinolone-resistant configures an emerging problem on public health, with importante implications in the antimicrobial therapeutic. In order to reduce the frequency of multiple-antibiotic resistant bacteria, epidemiologic surveillance and the rational use of antibiotic protocols need to be stablished. </SPAN></P></font></p><br><b>Palavras-chave: </b>&nbsp;urinary tract infection, quinolone, resistance</td></tr></table></tr></td></table></body></html>