ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:1731-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Fermentação e Biotecnologia ( Divisão J )</b><p align=justify><strong><P>PRODUÇÃO DE XILANASES POR CULTURAS DE <EM>TRICHOSPORON </EM>SP.</P></strong></p><p align=justify><b><u>Odacy Camilo </u></b> (<i>UFPE</i>); <b>Minelli Albuquerque </b> (<i>UFPE</i>); <b>Lidiane Roberta Cruz </b> (<i>UFPE</i>); <b>Elton Bill Amaral de Souza </b> (<i>UFPE</i>); <b>Sarah Priscila Paiva da Silva </b> (<i>UFPE</i>); <b>Cristina Maria Souza-motta </b> (<i>UFPE</i>); <b>Oliane Maria Correia Magalhães </b> (<i>UFPE</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2><P><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">Xilanases </SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">são enzimas de importante aplicação em diferentes setores industriais. </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvEPSTIM; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">Uma variedade de microrganismos, incluindo leveduras, fungos filamentosos e bactérias têm sido relatados como produtores de xilanases, sendo os fungos </SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">os mais importantes microrganismos utilizados pela indústria na produção desta enzima. </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">Novas amostras fúngicas devem ser identificadas, na busca de isolados com maior eficiência na produção desta enzima. O gênero </SPAN><I style="mso-bidi-font-style: normal"><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSX0370; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">Trichosporon </SPAN></I><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSX0370; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">apresenta células de leveduras e micélio verdadeiro, que se desarticulam formando artroconídios. Espécies de<I style="mso-bidi-font-style: normal"> </I></SPAN><I style="mso-bidi-font-style: normal"><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">Trichosporon</SPAN></I><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> têm sido relatadas como boas produtoras de xilanases.</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSX0369; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">O objetivo deste estudo foi selecionar culturas de <I style="mso-bidi-font-style: normal">Trichosporon</I> com a capacidade de produzir xilanases extracelulares. Foram utilizados 20 isolados de </SPAN><I><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSPH-R; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">Trichosporon</SPAN></I><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSPH-R; mso-bidi-font-style: italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> pertencentes a cinco diferentes espécies,</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> mantidas na Micoteca URM da Universidade Federal de Pernambuco. D</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSPH-R; mso-bidi-font-style: italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">iscos de 5mm das 20 c</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">ulturas crescidas </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSX0370; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">em á</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSPH-R; mso-bidi-font-style: italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">gar Sabouraud acrescido de 0,5% de extrato de levedura contido em placas de Petri, foram inoculados </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">em ágar-xilana contido em placa, a temperatura ambiente </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">(±<?xml:namespace prefix = st1 ns = "urn:schemas-microsoft-com:office:smarttags" /><st1:metricconverter w:st="on" ProductID="50 mM">28°C</st1:metricconverter>) e <st1:metricconverter w:st="on" ProductID="50 mM">37°C</st1:metricconverter> em B.O.D. durante sete dias, em </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSPH-R; mso-bidi-font-style: italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">triplicata</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">, para </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">avaliação quanto a capacidade de degradar xilana evidenciado pela formação de halo, e posterior seleção de culturas p</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSPH-R; mso-bidi-font-style: italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">ara determinar a produção de xilanases em meio de cultivo líquido contendo xilose, extrato de levedura e sais, inoculados com discos de 5mm da cultura transferidos para </SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">Erlenmeyer contendo 50mL do meio líquido, </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: AdvPSPH-R; mso-bidi-font-style: italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">incubados a </SPAN><st1:metricconverter w:st="on" ProductID="50 mM"><st1:metricconverter w:st="on" ProductID="32°C"><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">32°C</SPAN></st1:metricconverter><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">,</SPAN></st1:metricconverter><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> 160 rpm por 5 dias. </SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">A determinação da atividade xilanásica foi conduzida através da quantificação dos açúcares redutores gerados na presença de xilana <I style="mso-bidi-font-style: normal">birchwood</I> 1% em tampão fosfato de sódio (<st1:metricconverter w:st="on" ProductID="50 mM"><st1:metricconverter w:st="on" ProductID="50 mM">50 mM</st1:metricconverter>)</st1:metricconverter> pH 6.0, pelo método DNSA.</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> Das 20 culturas caracterizadas em meio sólido, 12</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> foram capazes de degradar a xilana e selecionadas para o cultivo líquido submerso.</SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-bidi-font-weight: bold; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> Todas as amostras testadas </SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">em meio líquido</SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-bidi-font-weight: bold; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> produziram enzimas xilanolíticas</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">, </SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-bidi-font-weight: bold; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">com atividade enzimática variando de <st1:metricconverter w:st="on" ProductID="1,78 a">1,78 a</st1:metricconverter> 24,25 U/mL.</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> </SPAN><I style="mso-bidi-font-style: normal"><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">T. cutaneum</SPAN></I><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> URM4789 f</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: SPSTimes-Italic; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">oi o </SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-bidi-font-weight: bold; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">maior produtor da xilanase extracelular</SPAN><SPAN style="FONT-FAMILY: Times-Roman; FONT-SIZE: 12pt; mso-fareast-language: PT-BR; mso-bidi-font-family: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> (24,25 U/mL). Baseado nos resultados, concluímos que espécies de <I style="mso-bidi-font-style: normal">Trichosporon</I> são promissoras para a produção de xilanases e <I style="mso-bidi-font-style: normal">T. cutaneum</I> URM4789 </SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-bidi-font-weight: bold; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">pode ser usado na indústria biotecnológica, sendo indicado para estudos de otimização da produção de xilanases.</SPAN></P> <P><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-bidi-font-weight: bold; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"></SPAN><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-bidi-font-weight: bold; mso-fareast-language: PT-BR; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">Apoio: FINEP Conv. 01.08.0392.00</P> <P style="TEXT-ALIGN: justify; MARGIN: 0cm 0cm 0pt; mso-layout-grid-align: none" class=MsoNormal><SPAN style="FONT-FAMILY: Times-Roman">Palavra chave: <EM>Trichosporon</EM>, caracterização,<EM> </EM>produção; xilanases. <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></SPAN></P></SPAN></font></p><br><b>Palavras-chave: </b>&nbsp;Trichosporon, Produção, caracterização, xilanases</td></tr></table></tr></td></table></body></html>