<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font  size=1>ResumoID:1707-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Microbiologia Clinica ( Divisão A )</b><p align=justify><strong>਀ 
<P CLASS="MSONORMAL" STYLE="TEXT-ALIGN: CENTER;" ALIGN="CENTER"><SPAN STYLE="">BACTERIAL RESISTANCE TO ANTIBIOTICS IN AQUATIC਀䔀一嘀䤀刀伀一䴀䔀一吀匀 伀䘀 刀䤀伀 䐀䔀 䨀䄀一䔀䤀刀伀⸀㰀伀㨀倀㸀㰀⼀伀㨀倀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀 
਀ 
਀㰀⼀猀琀爀漀渀最㸀㰀⼀瀀㸀㰀瀀 愀氀椀最渀㴀樀甀猀琀椀昀礀㸀㰀戀㸀㰀甀㸀䘀攀氀椀瀀攀 䠀攀爀渀愀渀搀攀猀 䌀漀甀琀椀渀栀漀 㰀⼀甀㸀㰀⼀戀㸀  ⠀㰀椀㸀唀䘀刀䨀㰀⼀椀㸀⤀㬀 㰀戀㸀刀椀挀愀爀搀漀  倀椀氀稀 嘀椀攀椀爀愀 㰀⼀戀㸀  ⠀㰀椀㸀唀䘀刀䨀㰀⼀椀㸀⤀㬀 㰀戀㸀䌀礀渀琀栀椀愀  䈀愀爀戀漀猀愀 匀椀氀瘀攀椀爀愀 㰀⼀戀㸀  ⠀㰀椀㸀唀䘀刀䨀㰀⼀椀㸀⤀㬀 㰀戀㸀嘀椀瘀椀愀渀  䄀氀瘀攀猀 䴀漀渀琀攀椀爀漀 㰀⼀戀㸀  ⠀㰀椀㸀唀䘀刀䨀㰀⼀椀㸀⤀㬀 㰀戀㸀刀漀搀漀氀瀀栀漀  䴀愀琀琀漀猀 䄀氀戀愀渀漀 㰀⼀戀㸀  ⠀㰀椀㸀唀䔀刀䨀㰀⼀椀㸀⤀㬀 㰀戀㸀伀爀氀愀渀搀漀  䈀漀渀椀昀挀椀漀 䴀愀爀琀椀渀猀 㰀⼀戀㸀  ⠀㰀椀㸀唀䘀刀䨀㰀⼀椀㸀⤀㰀戀爀㸀㰀戀爀㸀㰀⼀瀀㸀㰀戀㸀㰀昀漀渀琀 猀椀稀攀㴀㈀㸀刀攀猀甀洀漀㰀⼀昀漀渀琀㸀㰀⼀戀㸀㰀瀀 愀氀椀最渀㴀樀甀猀琀椀昀礀 挀氀愀猀猀㴀琀爀攀猀㸀㰀昀漀渀琀 猀椀稀攀㴀㈀㸀㰀搀椀瘀 猀琀礀氀攀㴀∀琀攀砀琀ⴀ愀氀椀最渀㨀 樀甀猀琀椀昀礀㬀∀㸀㰀昀漀渀琀 猀椀稀攀㴀∀㌀∀㸀㰀攀洀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㈀瀀琀㬀 昀漀渀琀ⴀ昀愀洀椀氀礀㨀 嘀攀爀搀愀渀愀㬀 昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀㬀∀ 氀愀渀最㴀∀䔀一ⴀ唀匀∀㸀䈀愀挀琀攀爀椀愀氀 爀攀猀椀猀琀愀渀挀攀 琀漀 愀渀琀椀戀椀漀琀椀挀猀 
is a public health issue, occurring due to the incorrect use of these਀猀甀戀猀琀愀渀挀攀猀 戀礀 瀀愀琀椀攀渀琀猀Ⰰ 琀栀甀猀 挀漀渀琀爀椀戀甀琀椀渀最 琀漀 琀栀攀 攀洀攀爀最攀渀挀攀 漀昀 椀渀挀爀攀愀猀椀渀最氀礀 
resistant strains. Recently, it has been reported that some soil bacterial਀猀琀爀愀椀渀猀 愀爀攀 爀攀猀椀猀琀愀渀琀 琀漀 猀攀瘀攀爀愀氀 愀渀琀椀戀椀漀琀椀挀猀 漀昀 挀氀椀渀椀挀愀氀 甀猀攀 戀漀琀栀 愀琀 挀氀椀渀椀挀愀氀 
concentrations and at fifty-fold higher concentrations. Some of these organisms਀愀爀攀 愀氀猀漀 挀愀瀀愀戀氀攀 漀昀 甀猀椀渀最 猀攀瘀攀爀愀氀 漀昀 琀栀攀猀攀 挀漀洀瀀漀甀渀搀猀 愀猀 琀栀攀椀爀 猀漀氀攀 挀愀爀戀漀渀 
source. In the present work, Bacteria from Guanabara bay water, a region that਀爀攀挀攀椀瘀攀猀 洀攀搀椀挀愀氀 愀渀搀 搀漀洀攀猀琀椀挀 眀愀猀琀攀Ⰰ 眀攀爀攀 椀猀漀氀愀琀攀搀 椀渀 洀攀搀椀愀 挀漀渀琀愀椀渀椀渀最 
ampicillin, tetracycline or kanamycin. Three species were identified by DNA਀猀攀焀甀攀渀挀椀渀最㨀 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㈀瀀琀㬀 昀漀渀琀ⴀ昀愀洀椀氀礀㨀 嘀攀爀搀愀渀愀㬀∀ 氀愀渀最㴀∀䔀一ⴀ唀匀∀㸀䄀攀爀漀洀漀渀愀猀 
sp., Acinetobacer sp. </span></em><em><span style="font-size: 12pt; font-family: Verdana; font-style: normal;" lang="EN-US">and a</span><span style="font-size: 12pt; font-family: Verdana;" lang="EN-US"> Klebsiella pneumoniae</span></em><em><span style="font-size: 12pt; font-family: Verdana; font-style: normal;" lang="EN-US">਀挀漀渀琀愀椀渀椀渀最 瀀氀愀猀洀椀搀椀愀氀 䐀一䄀 眀栀椀挀栀 挀漀渀昀攀爀猀 愀洀瀀椀挀椀氀氀椀渀 爀攀猀椀猀琀愀渀挀攀Ⰰ 愀猀 搀攀洀漀渀猀琀爀愀琀攀搀 
by transfecting this plasmid to originally non-resistant </span><span style="font-size: 12pt; font-family: Verdana;" lang="EN-US">E.coli</span></em><em><span style="font-size: 12pt; font-family: Verdana; font-style: normal;" lang="EN-US">.਀䘀漀氀氀漀眀椀渀最 琀栀椀猀Ⰰ 挀漀渀猀琀爀甀挀琀椀漀渀 漀昀 ㄀㘀匀 爀刀一䄀 氀椀戀爀愀爀椀攀猀 眀愀猀 瀀攀爀昀漀爀洀攀搀 昀漀爀 愀 
metagenomic analysis of resistant bacteria to increasing ampicillin਀挀漀渀挀攀渀琀爀愀琀椀漀渀猀 ⠀㈀　 洀最⼀䰀㬀 ㄀最⼀䰀 愀渀搀 ㄀㈀最⼀䰀⤀Ⰰ 椀渀 琀栀爀攀攀 愀焀甀愀琀椀挀 攀渀瘀椀爀漀渀洀攀渀琀猀 漀昀 刀椀漀 
de Janeiro’s coastal region with distinct input of sewer waste ( Barra da਀吀椀樀甀挀愀Ⰰ 琀栀攀 氀攀猀猀 椀洀瀀愀挀琀攀搀Ⰰ 䜀甀愀渀愀戀愀爀愀 戀愀礀 愀渀搀 䴀愀爀 䌀愀渀渀愀氀Ⰰ 琀栀攀 洀漀猀琀 椀洀瀀愀挀琀攀搀⤀Ⰰ 
bacterial growth was observed in all concentrations. This work allows the਀挀漀洀瀀爀攀栀攀渀猀椀漀渀 漀昀 琀栀攀 搀椀瘀攀爀猀椀琀礀 漀昀 爀攀猀椀猀琀愀渀琀 愀渀搀 猀甀瀀攀爀ⴀ爀攀猀椀猀琀愀渀琀 漀爀最愀渀椀猀洀猀 琀漀 
ampicillin, an antibiotic largely used in medical, veterinarial and laboratory਀瀀爀愀挀琀椀挀攀猀Ⰰ 愀渀搀 琀栀攀 瀀爀漀昀椀氀攀猀 漀昀 爀攀猀椀猀琀愀渀挀攀 琀漀 琀攀琀爀愀挀礀挀氀椀渀攀 愀渀搀 欀愀渀愀洀礀挀椀渀 椀渀 琀栀攀 
aquatic environment. Moreover, it helps to understand the pattern of dispersion਀漀昀 愀渀琀椀戀椀漀琀椀挀 爀攀猀椀猀琀愀渀挀攀 最攀渀攀猀 眀栀椀挀栀 洀愀礀 栀愀瀀瀀攀渀 氀愀爀最攀氀礀 戀礀 栀漀爀椀稀漀渀琀愀氀 最攀渀攀 
transfer between aquatic Bacteria, including pathogenic strains.</span></em></font></div>਀ 
</font></p><br><b>Palavras-chave: </b>&nbsp;Antibióticos, Aquático, Bactéria, Metagenômica, Resistência</td></tr></table></tr></td></table></body></html>਀�