25º Congresso Brasileiro de Microbiologia

25º Congresso Brasileiro de Microbiologia

ResumoID:1644-1

Área: Ecologia Microbiana ( Divisão I )

USE OF DGGE AND PCA ANALYSIS TO ASSESS EFFECTS &NBSP;OF CERATOCYSTIS FIMBRIATA ON BACTERIAL COMMUNITIES ASSOCIATED TO EUCALYPTUS

Anderson Ferreira (ESALQ-USP); Esteban Roberto Gonzáles (Suzano); Fernando Dini Andreote (Embrapa); João Lúcio Azevedo (ESALQ-USP); Welington Luiz Araújo (UMC)

Resumo

Brazil is one of the largest producers of Eucalyptus in the world, and the demand to increase the yield resulted to the expansion of the cultivation area. That resulted in changes of factors influencing eucalyptus cultivation and incidence of new phytopathogens. The disease caused by Ceratocystis fimbriata is emerging in reforestation areas and present as symptoms the cankers, generalized wilt, obstruction of roots and vases and plant desiccation. Nowadays, information about the possible effects of phytopathogens, like C. fimbriata, on host ecology and changes in the bacterial communities associated is limited. In that way, we used helthy and C. fimbriata infested Eucalyptus to assess the effects caused in the rhizoplane and endophytic bacterial communities. Total DNA was extracted from the rhyzoplane and endospher samples and the total bacterial diversity was assessed by culture-independent approach DGGE. Gel image analyses were carried out using the Gel compar II and Canoco softwares. Principal Component Analyses (PCA) based in the PCR-DGGE profiles showed that C. fimbriata present in Eucalyptus roots interfere on bacterial diversity communities. There are differences among treatments of rhyzoplane samples infested by C. fimbriata and healthy roots. When we assessed effects on endophytic communities diversity was observed differences between highly infested plants, intermediate infestation levels and healthy plants. PCA analysis didn't show differences between endophytic diversity in healthy plants and plants with intermediate infestation levels by C. fimbriata. Plant defense system activation, during the pathogen infestation process, can modulate these rhyzoplane and endophytic bacterial communities. The results obtained in this work showed can there are interactions between plant-associated microorganisms and C. fimbriata infestation. More studies should be focused in the application of endophytic and rhizoplane communities for biological control of C. fimbriata and identification of interaction genes.

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Financial Support: CNPq and Suzano Papel e Celulose.

Palavras-chave: MICROBIAL ECOLOGY, RHYZOPLANE, ENDOPHYTIC, 16 S rRNA

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