ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:1366-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Ecologia Microbiana ( Divisão I )</b><p align=justify><strong>ENDOPHYTIC FUNGAL COMMUNITY ASSOCIATED WITH THE DICOTYLEDONOUS PLANT <SPAN STYLE="FONT-STYLE: ITALIC;">COLOBANTHUS QUITENSIS</SPAN> (KUNTH) BARTL. (<SPAN STYLE="FONT-STYLE: ITALIC;">CARYOPHYLLACEAE</SPAN>) IN ANTARCTICA </strong></p><p align=justify><b>Luiz Henrique Rosa </b> (<i>UFOP</i>); <b>Mariana de Lourdes Ameida Vieira </b> (<i>UFMG</i>); <b><u>Iara Furtado Santiago </u></b> (<i>UFOP</i>); <b>Carlos Augusto Rosa </b> (<i>UFMG</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2><meta http-equiv="Content-Type" content="text/html; charset=utf-8"><meta name="ProgId" content="Word.Document"><meta name="Generator" content="Microsoft Word 11"><meta name="Originator" content="Microsoft Word 11"><link rel="File-List" href="file:///C:%5CDOCUME%7E1%5CADMINI%7E1%5CCONFIG%7E1%5CTemp%5Cmsohtml1%5C01%5Cclip_filelist.xml"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"></o:smarttagtype><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"></o:smarttagtype><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"></o:smarttagtype><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><!--[if !mso]><object classid="clsid:38481807-CA0E-42D2-BF39-B33AF135CC4D" id=ieooui></object> <style> st1:*{behavior:url(#ieooui) } </style> <![endif]--><style> <!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> </style><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} </style> <![endif]--> <p class="MsoNormal" style="line-height: 200%;"><span style="" lang="EN-GB">The distribution and diversity of fungal endophytes are described here in the leaves of <i>Colobanthus quitensis</i> (<i>Caryophylaceae</i>), </span><span lang="EN-GB">the only dicotyledonous plant present in <st1:place w:st="on">Antarctica</st1:place>. Five hundred ten leaf segments from 170 plant specimens were collected from six different sites at <st1:placename w:st="on">Admiralty</st1:placename> <st1:placetype w:st="on">Bay</st1:placetype> on <st1:placename w:st="on">King</st1:placename> <st1:placename w:st="on">George</st1:placename> <st1:placename w:st="on">Island</st1:placename>, in the <st1:place w:st="on"><st1:placename w:st="on">Antarctica</st1:placename> <st1:placetype w:st="on">Peninsula</st1:placetype></st1:place>. A total of 186 fungal isolates were obtained and identified by sequence analysis of the internal transcribed spacer region (ITS) of the rRNA gene. Sequence analyses of the ITS region revealed the presence of 19 different taxa represented by species from the genera <i style="">Aspergillus</i>, <i style=""><span style="color: black;">Cadophora</span></i><span style="color: black;">,<i style=""> </i></span><i style="">Davidiella</i>, <i style="">Entrophospora</i>, <i style="">Fusarium</i>, <i style="">Geomyces</i>, <i style="">Gyoerffyella</i>, <i style="">Microdochium</i>, <i style="">Mycocentrospora</i>, <i style="">Phaeosphaeria</i>, and nine </span><span style="line-height: 200%;" lang="EN-GB">unknown fungal taxa</span><span lang="EN-GB">. <i style="">Davidiella tassiana</i> was the prevalent species, followed by two possible new species of endophytic fungi, UFMGCB <span style="">2478 and 2668, present with 21.9, 16.4 and 14.4% abundances, respectively</span>. Twelve taxa <span style="">had low sequence identities when compared with the sequences of the fungi deposited in GenBank, and could represent new fungal species</span>. Four fungal endophytes recovered from <i style="">C. quitensis</i> were similar to species previously found in leaves or roots of the Antarctic grass <i style="">Deschampsia antartica</i> (<i style="">Poaceae</i>). In general, the diversity indexes obtained for the endophytic fungal community associated with <i style="">C. quitensis</i> were lower than those obtained for fungal communities of temperate and tropical plants. This work shows that <i style="">C. quitensis</i> is an interesting reservoir of saprobes and pathogenic and new endophytic fungal species, and could be a community model to further ecological and evolutionary studies as well as the study of mechanisms of adaptation of these microorganisms to extreme conditions.<span style=""><o:p></o:p></span></span></p> <p class="MsoNormal"><span lang="EN-GB"><o:p>&nbsp;</o:p></span></p> <p class="MsoNormal"><span lang="EN-GB">Apoio: CNPq, PROANTAR, MIDIAPI, FAPEMIG</span></p> </font></p><br><b>Palavras-chave: </b>&nbsp;Endophytic fungal, Distrbution and Diversity, Extreme environmental, Antarctica</td></tr></table></tr></td></table></body></html>