25º Congresso Brasileiro de Microbiologia

25º Congresso Brasileiro de Microbiologia

ResumoID:1339-1

Área: Micologia Médica ( Divisão B )

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Camila Maria de Sousa Lacerda Lacerda (CDTN); Estefânia Mara do Nascimento Martins Martins (UFMG); Maria Aparecida de Resende Resende (UFMG); Antero Silva Ribeiro de Andrade Andrade (CDTN)

Resumo
Sporotrichosis is a subacute or chronic infection caused by the fungus Sporothrix schenckii and the most common subcutaneous mycosis in Latin America. This mycosis develops mainly through penetration of the fungus into the dermis after wounds or abrasion of the skin produced by infected materials or by the inhalation of spores through the respiratory tract. Zoonotic transmission can occur after scratches or bites of animals, mainly cats, rodents, and armadillos.
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The cultures of S. schenckii, in solid medium, were irradiated at doses ranging from 1.0 to 9.0 kGy. After each dose the reproductive capacity, the viability and protein synthesis were estimated. The reproductive capacity was measured by the ability of the yeast cells to form colonies. The viability of irradiated cells was measured using the Kit FungaLight CFDA, AM / Propidium Iodide (Invitrogen) 2 and 24 hours after irradiation. The protein synthesis was analyzed by the incorporation of ³⁵S-methionine
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As a hole, our preliminary results point out that for the yeast cells of S. schenckii, it is possible to find an absorbed dose in which the pathogen loses its reproductive ability while retaining its viability and metabolism, a necessary condition for the development of a radioatenuated yeast vaccine.
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Palavras-chave: Sporothrix schenckii, vaccine, gamma radiation

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