ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:1282-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Micobacteriologa ( Divisão C )</b><p align=justify><strong> <P ALIGN="CENTER"><STRONG>ANTIBODIES TO THREE MYCOBACTERIAL RECOMBINANT ANTIGENS, EVALUATION IN CHILDREN WITH PULMONARY TUBERCULOSIS.</STRONG></P> </strong></p><p align=justify><b><u>Leonardo Silva de Araujo </u></b> (<i>FIOCRUZ</i>); <b>Terezinha M.martire </b> (<i>UNIRIO</i>); <b>Clemax C. Sant'anna </b> (<i>IPPMG/UFRJ</i>); <b>S. Lucena </b> (<i>HRPS</i>); <b>Ronir R. Luiz </b> (<i>FM/UFRJ</i>); <b>Marcus Barreto Conde </b> (<i>FM/UFRJ</i>); <b>Maria Helena Féres Saad </b> (<i>FIOCRUZ</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2> <div style="text-align: justify;"><span style="font-weight: bold;">Background: </span>In spite of the advent of several proposed modalities in molecular and immunological methods of diagnostic, the diagnosis of pulmonary tuberculosis in children still a problem due the paucity of the clinical specimen. Several commercial kits are available however none is used in routine diagnostic laboratory. A rapid, simple and relatively inexpensive diagnostic test will be crucial to future control efforts. Use of antibody detection ELISA seems to be an attractive strategy to aim children tuberculosis diagnosis. <br><span style="font-weight: bold;">Objective</span>: To evaluate the IgG immune response of children with tuberculosis to a panel of three Mycobacterium tuberculosis recombinant protein antigens MPT-64, ESAT-6 and CFP-10. Design: Sera of 207 children, of which 104 with respiratory symptoms (50 with pulmonary tuberculosis and 54 with non-tuberculosis pulmonary disease) and 103 controls without respiratory symptoms were tested by ELISA.<br><span style="font-weight: bold;">Results: </span>Ninety-eight percent of patients were BCG-vaccinated, all children with respiratory symptoms were HIV-negative and two TB cases had acid fast bacilli (AFB) smear positive. IgG-ELISA sensitivity was 27 %, 20 % and 16 %, respectively for MPT-64, ESAT-6 and CFP-10 antigens at 95% of specificity. The mean level of IgG reactivity for all antigens in TB patients was significantly higher than in controls (P&lt;0.001). Combining results of all antigens sensitivity increased to 48% followed by MPT-64+ESAT-6 = 40% MPT-64+CFP-10 = 39%, and ESAT-6+CFP-10 = 29%; although jeopardizing the specificity (86% and 90%, respectively). <br><span style="font-weight: bold;">Conclusion:</span> IgG-MPT-64 alone or plus ESAT-6 may be take into account as rapid adjuvant test to aim diagnosis of TB among suspected respiratory children, but other antigens must be evaluated to improve a multiplex ELISA for children pulmonary TB diagnosis.<br></div><br><link rel="File-List" href="file:///C:%5CDOCUME%7E1%5Cleonardo%5CCONFIG%7E1%5CTemp%5Cmsohtml1%5C01%5Cclip_filelist.xml"><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><style> <!-- /* Font Definitions */ @font-face {font-family:"Arial Narrow"; panose-1:2 11 5 6 2 2 2 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:647 0 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> </style><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} </style> <![endif]--> <p class="MsoNormal" style="margin-bottom: 10pt;"><strong><span style="font-family: &quot;Arial Narrow&quot;;">Financial Support:</span></strong><b><span style="font-family: &quot;Arial Narrow&quot;;"> </span></b><span style="font-family: &quot;Arial Narrow&quot;;">CNPq, DECIT/CNPq, FAPERJ, Programa Cooperação Brasil x Alemanha.</span><b><o:p></o:p></b></p> <br> </font></p><br><b>Palavras-chave: </b>&nbsp;Tuberculosis, Children, ELISA, Pulmonary TB, Humoral response</td></tr></table></tr></td></table></body></html>