<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font  size=1>ResumoID:1279-2</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Microbiologia de Alimentos ( Divisão K )</b><p align=justify><strong>DESENVOLVIMENTO DE MÉTODO PARA A QUANTIFICAÇÃO DIRETA DE <SPAN STYLE="FONT-STYLE: ITALIC;">SALMONELLA </SPAN>SP. POR PCR-TEMPO REAL਀ 
</strong></p><p align=justify><b>Hans Fröder </b>  (<i>FCF/USP</i>); <b>Mariza  Landgraf </b>  (<i>FCF/USP</i>); <b>Bernadette Dora Gombossy de Melo  Franco </b>  (<i>FCF/USP</i>); <b>Burkhard  Malorny </b>  (<i>BfR</i>); <b>Maria Teresa  Destro </b>  (<i>FCF/USP</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2>਀ 
਀ऀ㰀洀攀琀愀 栀琀琀瀀ⴀ攀焀甀椀瘀㴀∀䌀伀一吀䔀一吀ⴀ吀夀倀䔀∀ 挀漀渀琀攀渀琀㴀∀琀攀砀琀⼀栀琀洀氀㬀 挀栀愀爀猀攀琀㴀甀琀昀ⴀ㠀∀㸀㰀琀椀琀氀攀㸀䐀攀猀攀渀瘀漀氀瘀椀洀攀渀琀漀 搀攀 洀琀漀搀漀 瀀愀爀愀 愀 焀甀愀渀琀椀昀椀挀愀漀 搀椀爀攀琀愀 搀攀 匀愀氀洀漀渀㰀⼀琀椀琀氀攀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䜀䔀一䔀刀䄀吀伀刀∀ 挀漀渀琀攀渀琀㴀∀伀瀀攀渀伀昀昀椀挀攀⸀漀爀最 ㈀⸀㈀  ⠀䰀椀渀甀砀⤀∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䄀唀吀䠀伀刀∀ 挀漀渀琀攀渀琀㴀∀䠀愀渀猀∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䌀刀䔀䄀吀䔀䐀∀ 挀漀渀琀攀渀琀㴀∀㈀　　㤀　㠀㄀　㬀㤀㔀㌀　　　　∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䌀䠀䄀一䜀䔀䐀䈀夀∀ 挀漀渀琀攀渀琀㴀∀䠀愀渀猀∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䌀䠀䄀一䜀䔀䐀∀ 挀漀渀琀攀渀琀㴀∀㈀　　㤀　㠀㄀　㬀㄀㌀㐀㤀　　　　∀㸀 
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		@page { margin: 2cm }਀ऀऀ倀 笀 洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　⸀㈀㄀挀洀 紀 
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਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀　　─㬀∀ 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀 
<font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">1.਀䤀渀琀爀漀搀甀漀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀 
<p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify">਀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀䄀 
segunda geração de métodos que empregam a PCR,਀搀攀渀漀洀椀渀愀搀愀 倀䌀刀ⴀ琀攀洀瀀漀 爀攀愀氀 ⠀倀䌀刀ⴀ刀吀⤀Ⰰ 瘀攀洀 最愀渀栀愀渀搀漀 愀瀀爀漀瘀愀漀 
para detecção de patógenos, inclusive os de਀漀爀椀最攀洀 愀氀椀洀攀渀琀愀爀⸀ 䔀猀琀攀猀 洀琀漀搀漀猀 挀漀洀戀椀渀愀洀 愀猀 攀琀愀瀀愀猀 搀攀 
amplificação e detecção em uma única਀爀攀愀漀 攀洀 琀甀戀漀 昀攀挀栀愀搀漀Ⰰ 漀 焀甀攀 洀椀渀椀洀椀稀愀 漀 爀椀猀挀漀 搀攀 
contaminação da amostra. A quantificação਀搀攀 瀀愀琀最攀渀漀猀 攀洀 愀洀漀猀琀爀愀猀 搀攀 瀀爀漀搀甀琀漀猀 愀氀椀洀攀渀琀挀椀漀猀 琀攀洀 
se tornado cada vez mais importante para que se possa estabelecer, de਀洀愀渀攀椀爀愀 猀攀最甀爀愀 攀 挀漀渀昀椀瘀攀氀Ⰰ 漀 爀椀猀挀漀 搀愀焀甀攀氀攀 愀氀椀洀攀渀琀漀 攀洀 挀愀甀猀愀爀 
enfermidades transmitidas por alimentos. O objetivo do trabalho foi਀搀攀猀攀渀瘀漀氀瘀攀爀 甀洀 攀渀猀愀椀漀 瀀愀爀愀 焀甀愀渀琀椀昀椀挀愀爀 㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀㰀椀㸀匀愀氀洀漀渀攀氀氀愀㰀⼀椀㸀㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀 
em amostras de alimentos-modelo. </span></font></font></font>਀㰀⼀瀀㸀 
<p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify" lang="pt-BR">਀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㈀⸀ 
Material e Métodos</font></font></font></p>਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀　　─㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀 
<font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR">Caldos਀搀攀 挀愀爀渀攀 猀甀渀愀 攀 搀攀 愀瘀攀 昀漀爀愀洀 瀀爀攀瀀愀爀愀搀漀猀Ⰰ 挀漀氀攀琀愀搀漀猀 攀洀 琀甀戀漀猀 
estéreis e foram denominados alimento-modelo suíno e਀愀氀椀洀攀渀琀漀ⴀ洀漀搀攀氀漀 愀瘀攀⸀ 䄀氀焀甀漀琀愀猀 搀攀猀猀攀猀 愀氀椀洀攀渀琀漀猀ⴀ洀漀搀攀氀漀 昀漀爀愀洀 
artificialmente contaminadas com população elevada (&#8776;਀㘀 氀漀最 唀䘀䌀⼀洀䰀⤀ 攀 戀愀椀砀愀 ⠀☀⌀㠀㜀㜀㘀㬀 ㈀ 氀漀最 唀䘀䌀⼀洀䰀⤀ 搀攀 㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀㰀椀㸀匀愀氀洀漀渀攀氀氀愀㰀⼀椀㸀㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀 
Typhimurium DT 104, mantidas a   20 °C e a 8 °C e submetidas਀ 焀甀愀渀琀椀昀椀挀愀漀 搀漀 瀀愀琀最攀渀漀 攀洀 搀椀昀攀爀攀渀琀攀猀 
tempos pós-inoculação. DNA bacteriano foi਀攀砀琀爀愀搀漀Ⰰ 瀀甀爀椀昀椀挀愀搀漀 攀 昀漀椀 焀甀愀渀琀椀昀椀挀愀搀漀 攀洀瀀爀攀最愀渀搀漀ⴀ猀攀 甀洀 
ensaio </span></font></font></font><font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR"><i>Salmonella</i></span></font></font></font><font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR">-específico਀倀䌀刀ⴀ刀吀 甀琀椀氀椀稀愀渀搀漀 挀漀洀漀 愀氀瘀漀 漀 最攀渀攀 㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀㰀椀㸀琀甀昀㰀⼀椀㸀㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀⸀ 
Como controle empregou-se a enumeração de </span></font></font></font><font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR"><i>Salmonella</i></span></font></font></font><font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR">਀瀀漀爀 琀挀渀椀挀愀猀 挀漀渀瘀攀渀挀椀漀渀愀椀猀⸀ 㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀 
</p>਀ 
<p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify" lang="pt-BR">਀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㌀⸀ 
Resultados e Discussão</font></font></font></p>਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀　　─㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀 
<font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR">A਀焀甀愀渀琀椀昀椀挀愀漀 搀攀 㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀㰀椀㸀匀愀氀洀漀渀攀氀氀愀㰀⼀椀㸀㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀 
por PCR-RT apresentou limite de detecção &lt;1 log਀唀䘀䌀㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀猀甀戀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀攀焀 
</span></font></font></sub></font><font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR">e਀攀昀椀挀椀渀挀椀愀 搀攀 愀洀瀀氀椀昀椀挀愀漀 搀攀 㤀㐀─⸀ 一漀 猀攀 
verificou diferença (p&#8805;0,05) entre os métodos਀挀漀渀瘀攀渀挀椀漀渀愀氀 攀 洀漀氀攀挀甀氀愀爀 琀愀渀琀漀 瀀愀爀愀 瀀漀瀀甀氀愀攀猀 攀氀攀瘀愀搀愀猀 
como baixas em ambos os alimentos-modelo</span></font></font></font><font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR"><i>਀㰀⼀椀㸀㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀洀愀渀琀椀搀漀猀 
a 20 °C e para populações baixas mantidas a 8</span></font></font></font><font color="#000000"><sup><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR">o</span></font></font></sup></font><font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR">C.਀䔀渀琀爀攀琀愀渀琀漀Ⰰ 焀甀愀渀搀漀 愀猀 愀洀漀猀琀爀愀猀 挀漀洀 瀀漀瀀甀氀愀攀猀 攀氀攀瘀愀搀愀猀 
foram mantidas a 8° C, os métodos se comportaram de਀洀愀渀攀椀爀愀 搀椀昀攀爀攀渀琀攀 ⠀瀀☀氀琀㬀　Ⰰ　㔀⤀⸀㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀 
<p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify" lang="pt-BR">਀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㐀⸀ 
Conclusão</font></font></font></p>਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀　　─㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀 
<font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><span lang="pt-BR">O਀洀琀漀搀漀 倀䌀刀ⴀ刀吀 攀洀瀀爀攀最愀渀搀漀 挀漀洀漀 愀氀瘀漀 㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀㰀椀㸀琀甀昀㰀⼀椀㸀㰀⼀猀瀀愀渀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀猀瀀愀渀 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀Ⰰ 
mostrou-se potencialmente eficiente, mas ainda precisa ser submetido਀愀 渀漀瘀愀猀 愀瘀愀氀椀愀攀猀⸀ 䐀攀渀琀爀攀 愀猀 瘀愀渀琀愀最攀渀猀 搀愀 倀䌀刀ⴀ刀吀 
destacam-se a rapidez para a realização dos ensaios, a਀攀氀攀瘀愀搀愀 猀攀渀猀椀戀椀氀椀搀愀搀攀 攀 愀 瀀漀猀猀椀戀椀氀椀搀愀搀攀 搀攀 猀攀 琀爀愀戀愀氀栀愀爀 挀漀洀 瘀漀氀甀洀攀猀 
pequenos de amostra. A principal desvantagem do método é਀愀 搀攀琀攀挀漀 攀 愀 焀甀愀渀琀椀昀椀挀愀漀 搀攀 䐀一䄀 
proveniente de células bacterianas mortas.</span></font></font></font></p>਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀 氀椀渀攀ⴀ栀攀椀最栀琀㨀 ㄀　　─㬀∀ 氀愀渀最㴀∀瀀琀ⴀ䈀刀∀㸀 
<font color="#000000"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">5.਀䄀最爀愀搀攀挀椀洀攀渀琀漀猀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀 
<p class="western" style="margin-bottom: 0.35cm; line-height: 100%;" lang="pt-BR">਀㰀昀漀渀琀 挀漀氀漀爀㴀∀⌀　　　　　　∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀䌀䄀倀䔀匀Ⰰ 
CNPq e DAAD.</font></font></font></p>਀ 
਀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀倀愀氀愀瘀爀愀猀ⴀ挀栀愀瘀攀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀匀愀氀洀漀渀攀氀氀愀 猀瀀⸀Ⰰ 焀甀愀渀琀椀昀椀挀愀漀Ⰰ 倀䌀刀ⴀ琀攀洀瀀漀 爀攀愀氀Ⰰ 琀甀昀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀 