<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font  size=1>ResumoID:1279-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Microbiologia de Alimentos ( Divisão K )</b><p align=justify><strong>DESENVOLVIMENTO DE MÉTODO PARA A QUANTIFICAÇÃO DIRETA DE <SPAN STYLE="FONT-STYLE: ITALIC;">SALMONELLA</SPAN> SP. POR TRANSCRIPTASE REVERSA-PCR-TEMPO REAL਀ 
</strong></p><p align=justify><b>Hans Fröder </b>  (<i>FCF/USP</i>); <b>Mariza  Landgraf </b>  (<i>FCF/USP</i>); <b>Bernadette Dora Gombossy de Melo   Franco </b>  (<i>FCF/USP</i>); <b>Burkhard  Malorny </b>  (<i>BfR</i>); <b>Maria Teresa  Destro </b>  (<i>FCF/USP</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2>਀ 
਀ऀ㰀洀攀琀愀 栀琀琀瀀ⴀ攀焀甀椀瘀㴀∀䌀伀一吀䔀一吀ⴀ吀夀倀䔀∀ 挀漀渀琀攀渀琀㴀∀琀攀砀琀⼀栀琀洀氀㬀 挀栀愀爀猀攀琀㴀甀琀昀ⴀ㠀∀㸀㰀琀椀琀氀攀㸀䐀攀猀攀渀瘀漀氀瘀椀洀攀渀琀漀 搀攀 洀琀漀搀漀猀 瀀愀爀愀 愀 焀甀愀渀琀椀昀椀挀愀漀 搀椀爀攀琀愀 搀攀 匀愀氀洀漀㰀⼀琀椀琀氀攀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䜀䔀一䔀刀䄀吀伀刀∀ 挀漀渀琀攀渀琀㴀∀伀瀀攀渀伀昀昀椀挀攀⸀漀爀最 ㈀⸀㈀  ⠀䰀椀渀甀砀⤀∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䄀唀吀䠀伀刀∀ 挀漀渀琀攀渀琀㴀∀倀䄀匀䌀䠀伀䄀䰀∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䌀刀䔀䄀吀䔀䐀∀ 挀漀渀琀攀渀琀㴀∀㈀　　㤀　㠀㄀　㬀㤀㌀㔀　　　　∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䌀䠀䄀一䜀䔀䐀䈀夀∀ 挀漀渀琀攀渀琀㴀∀䠀愀渀猀∀㸀㰀洀攀琀愀 渀愀洀攀㴀∀䌀䠀䄀一䜀䔀䐀∀ 挀漀渀琀攀渀琀㴀∀㈀　　㤀　㠀㄀　㬀㄀㌀㐀㤀　　　　∀㸀 
	਀ऀ 
	਀ऀ 
	਀ऀ 
	<style type="text/css">਀ऀ㰀℀ⴀⴀ 
		@page { margin: 2cm }਀ऀऀ倀 笀 洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　⸀㈀㄀挀洀 紀 
	-->਀ऀ㰀⼀猀琀礀氀攀㸀 
਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㄀⸀ 
Introdução</font></font></p>਀ 
<p class="western" style="margin-bottom: 0cm;" align="justify"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">Para਀漀戀琀攀爀 爀攀猀甀氀琀愀搀漀猀 爀瀀椀搀漀猀 攀 挀漀渀昀椀瘀攀椀猀 焀甀攀 瀀攀爀洀椀琀愀洀 漀 
monitoramento da segurança microbiológica de alimentos,਀焀甀攀爀 猀攀樀愀 瀀攀氀愀 椀渀搀切猀琀爀椀愀 漀甀 瀀攀氀漀猀 爀最漀猀 搀攀 
fiscalização, diversos métodos alternativos têm਀猀椀搀漀 搀攀猀攀渀瘀漀氀瘀椀搀漀猀 瀀愀爀愀 愀 搀攀琀攀挀漀 攀 焀甀愀渀琀椀昀椀挀愀漀 
de </font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><i>Salmonella</i></font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">.਀䴀琀漀搀漀猀 焀甀攀 攀洀瀀爀攀最愀洀 愀 倀䌀刀ⴀ琀攀洀瀀漀 爀攀愀氀 ⠀倀䌀刀ⴀ刀吀⤀ 瘀攀洀 最愀渀栀愀渀搀漀 
aprovação para detecção de patógenos,਀椀渀挀氀甀猀椀瘀攀 漀猀 搀攀 漀爀椀最攀洀 愀氀椀洀攀渀琀愀爀⸀ 㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀䄀 
transcriptase reversa-PCR (RT-PCR) tem sido empregada em conjunto com਀倀䌀刀ⴀ刀吀Ⰰ 瀀攀爀洀椀琀椀渀搀漀 愀 搀攀琀攀挀漀 攀 愀 焀甀愀渀琀椀昀椀挀愀漀 
de mRNA de uma única célula. </font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">O਀瀀爀漀瀀猀椀琀漀 搀漀 攀猀琀甀搀漀 昀漀椀 搀攀猀攀渀瘀漀氀瘀攀爀 甀洀 攀渀猀愀椀漀 瀀愀爀愀 焀甀愀渀琀椀昀椀挀愀爀 
</font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><i>Salmonella</i></font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">਀戀愀猀攀愀搀漀 渀愀 琀爀愀渀猀挀爀椀瀀琀愀猀攀 爀攀瘀攀爀猀愀ⴀ倀䌀刀ⴀ琀攀洀瀀漀 爀攀愀氀 ⠀刀吀ⴀ倀䌀刀ⴀ刀吀⤀⸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀 
਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㈀⸀ 
Material e Métodos</font></font></p>਀ 
<p class="western" style="margin-bottom: 0cm;" align="justify"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">Caldos਀搀攀 挀愀爀渀攀 猀甀渀愀 攀 搀攀 愀瘀攀 昀漀爀愀洀 瀀爀攀瀀愀爀愀搀漀猀Ⰰ 挀漀氀攀琀愀搀漀猀 攀洀 琀甀戀漀猀 
estéreis e foram denominados alimento-modelo suíno e਀愀氀椀洀攀渀琀漀ⴀ洀漀搀攀氀漀 愀瘀攀⸀ 䄀氀焀甀漀琀愀猀 搀攀猀猀攀猀 愀氀椀洀攀渀琀漀猀ⴀ洀漀搀攀氀漀 昀漀爀愀洀 
artificialmente contaminadas com população elevada (&#8776;਀㘀 氀漀最 唀䘀䌀⼀洀䰀⤀ 攀 戀愀椀砀愀 ⠀☀⌀㠀㜀㜀㘀㬀 ㈀ 氀漀最 唀䘀䌀⼀洀䰀⤀ 搀攀 㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀椀㸀匀愀氀洀漀渀攀氀氀愀㰀⼀椀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀 
Typhimurium DT 104, mantidas a 20 °C e a 8 °C e submetidas à਀焀甀愀渀琀椀昀椀挀愀漀 搀漀 瀀愀琀最攀渀漀 攀洀 搀椀昀攀爀攀渀琀攀猀 琀攀洀瀀漀猀 
pós-inoculação. Após estabilização਀挀攀氀甀氀愀爀Ⰰ 攀砀琀爀愀漀 攀 瀀甀爀椀昀椀挀愀漀Ⰰ 漀 刀一䄀 
total isolado foi quantificado por RT-PCR-RT utilizando como alvo o਀最攀渀攀 㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀椀㸀琀甀昀㰀⼀椀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀 
num ensaio </font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><i>Salmonella</i></font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">-específico਀搀攀猀攀渀瘀漀氀瘀椀搀漀 渀攀猀猀攀 攀猀琀甀搀漀⸀ 䌀漀洀漀 挀漀渀琀爀漀氀攀 攀洀瀀爀攀最漀甀ⴀ猀攀 愀 攀渀甀洀攀爀愀漀 
de </font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><i>Salmonella</i></font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">਀瀀漀爀 琀挀渀椀挀愀猀 挀漀渀瘀攀渀挀椀漀渀愀椀猀⸀ 㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀 
</p>਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀琀攀砀琀ⴀ椀渀搀攀渀琀㨀 ㄀⸀㈀㔀挀洀㬀 洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀 
<br>਀㰀⼀瀀㸀 
<p class="western" style="margin-bottom: 0cm;" align="justify"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">3.਀刀攀猀甀氀琀愀搀漀猀 攀 䐀椀猀挀甀猀猀漀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀 
<p class="western" style="margin-bottom: 0cm;" align="justify"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">	</font></font></p>਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀䄀 
quantificação de </font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><i>Salmonella</i></font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">਀瀀漀爀 刀吀ⴀ倀䌀刀ⴀ刀吀 愀瀀爀攀猀攀渀琀漀甀 氀椀洀椀琀攀 搀攀 搀攀琀攀挀漀 搀攀 ㈀ 氀漀最 
UFC</font></font><sub><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">eq</font></font></sub><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">਀攀 攀昀椀挀椀渀挀椀愀 搀攀 愀洀瀀氀椀昀椀挀愀漀 搀攀 ㄀　　─⸀ 一漀 
houve diferença significativa </font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">entre਀漀猀 爀攀猀甀氀琀愀搀漀猀 漀戀琀椀搀漀猀 瀀攀氀漀 洀琀漀搀漀 挀漀渀瘀攀渀挀椀漀渀愀氀 攀 漀 洀漀氀攀挀甀氀愀爀 
(p&#8805;0,05) tanto para</font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">਀瀀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀漀瀀甀氀愀漀 
elevada como para baixa em ambos os alimentos-modelo</font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><i>਀㰀⼀椀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀洀愀渀琀椀搀漀猀 
a 20 °C e para os alimentos-modelo mantidos a 8</font></font><sup><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">o</font></font></sup><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">C਀攀 挀漀洀 瀀漀瀀甀氀愀漀 戀愀椀砀愀⸀ 倀漀爀 漀甀琀爀漀 氀愀搀漀Ⰰ 栀漀甀瘀攀 搀椀昀攀爀攀渀愀 
significativa (p&lt;0,05) para população elevada nas਀愀洀漀猀琀爀愀猀 洀愀渀琀椀搀愀猀 愀 㠀뀀 䌀⸀ 伀戀猀攀爀瘀漀甀ⴀ猀攀 琀愀洀戀洀 焀甀攀 
alimentos-modelo mantidos a 20 °C apresentaram uma queda mais਀爀瀀椀搀愀 渀愀 攀砀瀀爀攀猀猀漀 搀攀 㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀椀㸀琀甀昀 
</i></font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">do਀焀甀攀 愀焀甀攀氀攀猀 洀愀渀琀椀搀漀猀 愀 㠀 뀀䌀⸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀 
਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㐀⸀ 
Conclusão</font></font></p>਀ 
<p class="western" style="margin-bottom: 0cm;" align="justify"><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">O਀洀琀漀搀漀 刀吀ⴀ倀䌀刀ⴀ刀吀 攀洀瀀爀攀最愀渀搀漀 㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀㰀椀㸀琀甀昀㰀⼀椀㸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀 
apresenta potencial para uso, mas ainda precisa ser submetido a novas਀愀瘀愀氀椀愀攀猀 攀 愀瀀爀椀洀漀爀愀洀攀渀琀漀Ⰰ 愀 昀椀洀 搀攀 戀甀猀挀愀爀 洀攀氀栀漀爀椀愀 渀漀 
limite de detec</font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">ção.਀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀昀漀渀琀 昀愀挀攀㴀∀䄀爀椀愀氀Ⰰ 猀愀渀猀ⴀ猀攀爀椀昀∀㸀㰀昀漀渀琀 猀琀礀氀攀㴀∀昀漀渀琀ⴀ猀椀稀攀㨀 ㄀㄀瀀琀㬀∀ 猀椀稀攀㴀∀㈀∀㸀吀愀洀戀洀 
é importante que seja avaliada a meia-vida do </font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2"><i>tuf</i></font></font><font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">-RNA,਀瀀愀爀愀 焀甀攀 猀攀 瀀漀猀猀愀 焀甀愀渀琀椀昀椀挀愀爀 猀漀洀攀渀琀攀 刀一䄀 搀攀 挀氀甀氀愀猀 瘀椀瘀攀椀猀⸀ 
A desvantagem deste método é a conversão de RNA਀瀀愀爀愀 挀䐀一䄀Ⰰ 焀甀攀 瀀漀搀攀 挀漀渀琀爀椀戀甀椀爀 瀀愀爀愀 愀猀 瘀愀爀椀愀攀猀 攀 愀 
falta de reprodutibilidade devido ao estado dinâmico das਀挀氀甀氀愀猀Ⰰ 愀 焀甀愀氀椀搀愀搀攀 瘀愀爀椀瘀攀氀 搀漀 刀一䄀 瀀甀爀椀昀椀挀愀搀漀 攀 愀 
disponibilidade do molde para a conversão.  </font></font>਀㰀⼀瀀㸀 
਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀琀攀砀琀ⴀ椀渀搀攀渀琀㨀 ⴀ　⸀　㌀挀洀㬀 洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀 
<font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">5.਀䄀最爀愀搀攀挀椀洀攀渀琀漀猀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀 
਀㰀瀀 挀氀愀猀猀㴀∀眀攀猀琀攀爀渀∀ 猀琀礀氀攀㴀∀琀攀砀琀ⴀ椀渀搀攀渀琀㨀 ⴀ　⸀　㌀挀洀㬀 洀愀爀最椀渀ⴀ戀漀琀琀漀洀㨀 　挀洀㬀∀ 愀氀椀最渀㴀∀樀甀猀琀椀昀礀∀㸀 
<font face="Arial, sans-serif"><font style="font-size: 11pt;" size="2">	CAPES,਀䌀一倀焀 攀 䐀䄀䄀䐀⸀㰀⼀昀漀渀琀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀 
<p class="western" style="margin-bottom: 0cm;"><br>਀㰀⼀瀀㸀 
਀ 
</font></p><br><b>Palavras-chave: </b>&nbsp;Salmonella sp., quantificação, transcriptase reversa-PCR-tempo real, tuf</td></tr></table></tr></td></table></body></html>਀�