ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:1196-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Micologia Médica ( Divisão B )</b><p align=justify><strong>IDENTIFICAÇÃO DOS TIPOS MOLECULARES DOS AGENTES DA CRIPTOCOCOSE CIRCULANTES NO RIO DE JANEIRO</strong></p><p align=justify><b><u>Glaucia Gonçalves Barbosa </u></b> (<i>FIOCRUZ/IPEC</i>); <b>Luciana Trilles </b> (<i>FIOCRUZ/IPEC</i>); <b>Márcia dos Santos Lazéra </b> (<i>FIOCRUZ/IPEC</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">A criptococose coloca-se entre as infecções fúngicas humanas de significativa letalidade e morbidade, principalmente sob forma de meningoencefalite, seja em imunodeprimidos ou em indivíduos imunocompetentes, sendo causada pelos basidiomicetos <I style="mso-bidi-font-style: normal">Cryptococcus neoformans</I> e <I style="mso-bidi-font-style: normal">C. gattii</I>.</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> </SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; LETTER-SPACING: -0.15pt; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">No Brasil, estudos clínico-epidemiológicos mostram a importância da criptococose <I>gattii</I> do SNC em adultos jovens de ambos os sexos e crianças nas regiões N e NE, com letalidade que varia entre 35% a 40%.</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; LETTER-SPACING: -0.15pt; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> </SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">O maior surto foi registrado na ilha de Vancouver, Canadá, atingindo 38 casos humanos, entre 1999 e 2001, a maioria imunocompetente, com letalidade em torno de 10</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">%. </SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">No Rio de Janeiro, estudos moleculares de isolados clínicos de criptococose associada a AIDS demonstraram o predomínio do tipo VNI, seguido do VNII. Ainda não foi identificado nenhum isolado ambiental VGII na região, porém, tivemos oportunidade de identificar isolado clínico de criptococose por <I>C. gattii</I></SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-bidi-font-style: italic; mso-fareast-theme-font: minor-latin">: p</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">aciente de sexo feminino, residente no Flamengo há longos anos,</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> sem histórico de viagem a área endêmica,</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> HIV negativo, que iniciou o quadro com cefaléia em capacete, seguido de desorientação, e perda de força muscular generalizada. O isolamento do agente de LCR revelou se tratar de <I>C. gattii</I></SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> tipo molecular VGII</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">. A paciente é acompanhada </SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">em nosso</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> ambulatório, c</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">om</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> seqüelas graves e uso de muletas.</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> Com isso, pretendemos i</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">dentificar, no ambiente, a possível fonte de infecção da criptococose <I>gattii</I> no Rio de Janeiro a partir do caso índice.</SPAN><B style="mso-bidi-font-weight: normal"><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> </SPAN></B><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">O processamento das amostras ambientais foi feito por </SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA">plaqueado em meio Agar semente de níger (NSA) </SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: PT-BR; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA">suplementado com ant</SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: PT-BR; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">ibióticos e</SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA"> incubação a 25ºC.</SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> </SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: PT-BR; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA">A espécie </SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: PT-BR; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">foi</SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: PT-BR; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA"> determinada utilizando o meio CGB.</SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: PT-BR; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> Após a e</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">xtração de DNA,</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> </SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">foi</SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA"> </SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">feita PCR utilizando </SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-bidi-font-style: italic; mso-fareast-theme-font: minor-latin">os</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> </SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">primer</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">s</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA"> do gene <I style="mso-bidi-font-style: normal">URA5</I> senso (5 ATGTCCTCCCAAGCCCTCGAC3 ) e anti-senso (5 TTAAGACCT CTGAACACCGTACTC3 ). A</SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin"> </SPAN><SPAN lang=PT style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT; mso-bidi-language: AR-SA">caracterização molecular foi feita pelo método de </SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">RFLP do gene <I>URA5</I></SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-bidi-font-style: italic; mso-fareast-theme-font: minor-latin"> utilizando as </SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-bidi-font-weight: bold; mso-fareast-language: PT-BR; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">enzimas de restrição </SPAN><I><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">Hha</SPAN></I><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">I e <I>Sa</I></SPAN><I><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">u</SPAN></I><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA">96I. </SPAN><SPAN style="FONT-SIZE: 12pt; LINE-HEIGHT: 115%; FONT-FAMILY: 'Arial','sans-serif'; mso-fareast-language: EN-US; mso-fareast-font-family: Calibri; mso-ansi-language: PT-BR; mso-bidi-language: AR-SA; mso-fareast-theme-font: minor-latin">Foram feitas 4 coletas (3 no entorno da casa e 1 no entorno do trabalho da paciente). Até o momento, foram isoladas 700 colônias: 393 <I style="mso-bidi-font-style: normal">C. gattii</I> tipo molecular VGI e 07 <I style="mso-bidi-font-style: normal">C. neoformans</I> tipo molecular VNI. Falta ainda a análise de 300 colônias.</SPAN></font></p><br><b>Palavras-chave: </b>&nbsp;Cryptococcus gattii, Rio de Janeiro, Tipagem molecular</td></tr></table></tr></td></table></body></html>