DETECTION OF GIARDIA DUODENALIS GENOTYPE AII IN WELL WATER FROM A PERIURBAN COMMUNITY LOCATED IN SUZANO, SÃO PAULO.
Licia Natal Fernandes (FSP/USP); Patricia Pereira de Souza (FSP/USP); Ronalda Silva de Araújo (FSP/USP); Milena Dropa (FSP/USP); Lívia Carminato Balsalobre (FSP/USP); Kélvilin Anahí Gonzales Sábio (FSP/USP); Maria Tereza Peppe Razzolini (FSP/USP); Glavur Rogério Matté (FSP/USP); Maria Helena Matté (FSP/USP)
Resumo
㰀⼀猀琀礀氀攀㸀㰀℀ⴀⴀ嬀椀昀 最琀攀 洀猀漀 崀㸀
㰀℀嬀攀渀搀椀昀崀ⴀⴀ㸀
㰀瀀 挀氀愀猀猀㴀∀䴀猀漀一漀爀洀愀氀∀ 猀琀礀氀攀㴀∀琀攀砀琀ⴀ愀氀椀最渀㨀 樀甀猀琀椀昀礀㬀∀㸀䜀椀愀爀搀椀愀 搀甀漀搀攀渀愀氀椀猀 椀猀 愀 瀀爀漀琀漀稀漀愀渀
that parasitizes humans and other mammals and can cause giardiasis. Although椀琀猀 椀猀漀氀愀琀攀猀 栀愀瘀攀 戀攀攀渀 搀椀瘀椀搀攀搀 椀渀琀漀 猀攀瘀攀渀 䄀猀猀攀洀戀氀愀最攀猀Ⰰ 渀愀洀攀搀 䄀 琀漀 䜀Ⰰ 漀渀氀礀 䄀 愀渀搀
B have been detected in human feces. Assemblege A isolates are commonly divided椀渀琀漀 琀眀漀 最攀渀漀琀礀瀀攀猀Ⰰ 䄀䤀 愀渀搀 䤀䤀⸀ 䤀渀 㰀猀琀㨀挀漀甀渀琀爀礀ⴀ爀攀最椀漀渀㸀㰀猀琀㨀瀀氀愀挀攀㸀䈀爀愀稀椀氀㰀⼀猀琀㨀瀀氀愀挀攀㸀㰀⼀猀琀㨀挀漀甀渀琀爀礀ⴀ爀攀最椀漀渀㸀
there is scarce information about the presence of this protozoan in well water.䄀猀 琀栀椀猀 洀愀琀爀椀挀攀 椀猀 甀猀攀搀 戀礀 洀愀渀礀 挀漀洀洀甀渀椀琀椀攀猀 愀猀 猀漀甀爀挀攀 漀昀 眀愀琀攀爀 昀漀爀 栀甀洀愀渀
consumption, it is important to verify the presence of this parasite in these猀愀洀瀀氀攀猀Ⰰ 愀渀搀 搀攀琀攀挀琀 眀栀椀挀栀 愀猀猀攀洀戀氀愀最攀猀 洀椀最栀琀 戀攀 瀀爀攀猀攀渀琀Ⰰ 眀栀椀挀栀 挀愀渀 漀渀氀礀 戀攀 搀漀渀攀
by the employment of molecular techniques. Three samples (10L) of well water眀攀爀攀 挀漀氀氀攀挀琀攀搀 椀渀 瀀攀爀椀甀爀戀愀渀 挀漀洀洀甀渀椀琀椀攀猀 氀漀挀愀琀攀搀 椀渀 琀栀攀 㰀猀琀㨀瀀氀愀挀攀㸀㰀猀琀㨀瀀氀愀挀攀琀礀瀀攀㸀洀甀渀椀挀椀瀀愀氀椀琀礀㰀⼀猀琀㨀瀀氀愀挀攀琀礀瀀攀㸀
of Suzano (SP) and concentrated by洀漀搀椀昀椀攀搀 洀攀洀戀爀愀渀攀 昀椀氀琀爀愀琀椀漀渀 琀攀挀栀渀椀焀甀攀⸀ 吀眀漀 漀昀 琀栀攀 猀愀洀瀀氀攀猀 眀攀爀攀 愀氀猀漀 瀀甀爀椀昀椀攀搀
before DNA was extracted. A nested PCR that amplifies a 890bp of gdh gene was瀀爀漀挀攀攀搀攀搀⸀ 倀漀猀椀琀椀瘀攀 猀愀洀瀀氀攀猀 眀攀爀攀 挀氀漀渀攀搀 愀渀搀 猀攀焀甀攀渀挀攀搀⸀ 倀栀礀氀漀最攀渀攀琀椀挀 愀渀愀氀礀猀攀 眀愀猀
developed by Mega 4.1 beta program. Two out of three well samples were挀漀渀昀椀爀洀攀搀 琀漀 戀攀 瀀漀猀椀琀椀瘀攀 昀漀爀 䜀椀愀爀搀椀愀 搀甀漀搀攀渀愀氀椀猀⸀ 吀栀攀 漀渀氀礀 渀攀最愀琀椀瘀攀 猀愀洀瀀氀攀 搀椀搀
not go through purification step which indicates that this might be important琀漀 爀攀洀漀瘀攀 倀䌀刀 椀渀栀椀戀椀琀漀爀猀Ⰰ 昀爀攀焀甀攀渀琀氀礀 瀀爀攀猀攀渀琀 椀渀 眀愀琀攀爀 猀愀洀瀀氀攀猀⸀ 倀栀礀氀漀最攀渀攀琀椀挀
tree placed the sequences obtained in this work with the genotype AII sequences昀爀漀洀 䜀攀渀戀愀渀欀 甀猀攀搀 愀猀 爀攀昀攀爀攀渀挀攀 猀琀爀愀椀渀猀⸀ 䄀猀 琀栀椀猀 最攀渀漀琀礀瀀攀 椀猀 挀漀洀洀漀渀氀礀 愀猀猀漀挀椀愀琀攀搀
to human giardiasis, the contact with the well water from these comunities represents愀 爀椀猀欀 昀漀爀 瀀甀戀氀椀挀 栀攀愀氀琀栀⸀ 吀栀攀爀攀昀漀爀攀Ⰰ 琀栀攀 眀攀氀氀 眀愀琀攀爀 猀栀漀甀氀搀 甀渀搀攀爀最漀 琀漀 猀漀洀攀
treatment process before its consumption and more attention should be given to琀栀椀猀 欀椀渀搀 漀昀 眀愀琀攀爀 椀渀 漀爀搀攀爀 琀漀 愀瘀漀椀搀 挀漀渀琀愀洀椀渀愀琀椀漀渀⸀ 䄀瀀漀椀漀㨀 䘀椀渀攀瀀 攀 䌀一倀焀⸀ 㰀猀瀀愀渀 猀琀礀氀攀㴀∀∀㸀☀渀戀猀瀀㬀☀渀戀猀瀀㬀㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀∀㸀☀渀戀猀瀀㬀☀渀戀猀瀀㬀☀渀戀猀瀀㬀㰀⼀猀瀀愀渀㸀㰀⼀瀀㸀
㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀倀愀氀愀瘀爀愀猀ⴀ挀栀愀瘀攀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀最攀渀漀琀礀瀀椀渀最Ⰰ 䜀椀愀爀搀椀愀Ⰰ 眀攀氀氀 眀愀琀攀爀Ⰰ 瀀甀戀氀椀挀 栀攀愀氀琀栀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀