| Área: Micobacteriologa ( Divisão C ) GENOTYPING OF MYCOBACTERIUM LEPRAE FROM BRAZILIAN LEPROSY PATIENTS
Amanda Nogueira Brum Fontes (FIOCRUZ); Rama Murthy Sakamuri Sakamuri (CSU); Ida Maria F. Dias Baptista Baptista (ILSL); Somei Ura Ura (ILSL); Harrison Magdinier Gomes Gomes (FIOCRUZ); Edson Cláudio A. de Albuquerque Albuquerque (FIOCRUZ); Milton Ozório Moraes Moraes (FIOCRUZ); Alejandra Nóbrega Martinez Martinez (FIOCRUZ); Euzenir Nunes Sarno Sarno (FIOCRUZ); Philip Noel Suffys Suffys (FIOCRUZ); Varalakshmi D. Vissa Vissa (CSU); Patrick J. Brennan Brennan (CSU); Denise Silva Rezende Rezende (UNIR); Maria Manuela da F. Moura Moura (UNIR)
ResumoIntroduction. Leprosy is caused by infection with Mycobacterium leprae, an obligate intracellular parasite and it remains a major health problem in several developing countries. Since 2000, variable number of tandem repeats (VNTR) have been exploited in the M. leprae genome and in 2005, three single nucleotide polymorphism (SNP) were also discovered to be useful for analysis of the global spread of leprosy. Nowadays the detection of strain variation in areas with high prevalence of leprosy is possible, thus allowing more profound transmission and phylogeny studies. 㰀倀 挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀 猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀 挀洀 挀洀 瀀琀㬀 䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀 ㈀ ─㬀 吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀 樀甀猀琀椀昀礀∀㸀㰀䈀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀 渀漀爀洀愀氀∀㸀㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀㰀匀倀䄀一 氀愀渀最㴀䔀一ⴀ唀匀 猀琀礀氀攀㴀∀䘀伀一吀ⴀ匀䤀娀䔀㨀 瀀琀㬀 䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀 ㈀ ─㬀 䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 䄀爀椀愀氀㬀 洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ昀漀渀琀ⴀ昀愀洀椀氀礀㨀 ✀吀椀洀攀猀 一攀眀 刀漀洀愀渀✀㬀 洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀䴀攀琀栀漀搀猀㰀⼀匀倀䄀一㸀㰀⼀䤀㸀㰀⼀䈀㸀㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀㰀匀倀䄀一 氀愀渀最㴀䔀一ⴀ唀匀 猀琀礀氀攀㴀∀䘀伀一吀ⴀ匀䤀娀䔀㨀 瀀琀㬀 䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀 ㈀ ─㬀 䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 䄀爀椀愀氀㬀 洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ昀漀渀琀ⴀ昀愀洀椀氀礀㨀 ✀吀椀洀攀猀 一攀眀 刀漀洀愀渀✀㬀 洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀⸀㰀⼀匀倀䄀一㸀㰀⼀䤀㸀㰀匀倀䄀一 氀愀渀最㴀䔀一ⴀ唀匀 猀琀礀氀攀㴀∀䘀伀一吀ⴀ匀䤀娀䔀㨀 瀀琀㬀 䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀 ㈀ ─㬀 䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 䄀爀椀愀氀㬀 洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ昀漀渀琀ⴀ昀愀洀椀氀礀㨀 ✀吀椀洀攀猀 一攀眀 刀漀洀愀渀✀㬀 洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀 䤀渀 琀栀椀猀 猀琀甀搀礀Ⰰ 眀攀 瀀爀攀猀攀渀琀 搀愀琀愀 漀戀琀愀椀渀攀搀 戀愀猀攀搀 漀渀 攀砀愀洀椀渀愀琀椀漀渀 漀昀 琀栀攀 愀氀氀攀氀椀挀 搀椀瘀攀爀猀椀琀礀 漀昀 㘀 猀攀氀攀挀琀 嘀一吀刀 愀渀搀 琀栀爀攀攀 匀一倀 氀漀挀椀 椀渀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䴀⸀ 氀攀瀀爀愀攀 㰀⼀䤀㸀最攀渀漀洀攀⸀㰀匀倀䄀一 猀琀礀氀攀㴀∀洀猀漀ⴀ猀瀀愀挀攀爀甀渀㨀 礀攀猀∀㸀☀渀戀猀瀀㬀 㰀⼀匀倀䄀一㸀䌀氀椀渀椀挀愀氀 椀猀漀氀愀琀攀猀 漀戀琀愀椀渀攀搀 昀爀漀洀 猀欀椀渀 戀椀漀瀀猀椀攀猀 猀愀洀瀀氀攀猀 愀渀搀 娀椀攀栀氀ⴀ一攀攀氀猀攀渀 猀琀愀椀渀攀搀 洀椀挀爀漀猀挀漀瀀椀挀 猀氀椀搀攀猀 昀爀漀洀 刀椀漀 搀攀 䨀愀渀攀椀爀漀Ⰰ 匀漀 倀愀甀氀漀 愀渀搀 刀漀渀搀渀椀愀 眀攀爀攀 愀渀愀氀礀稀攀搀 ⠀渀㴀㌀㌀⤀⸀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀
Results and conclusions. Using a PCR-RFLP-based procedure, the characterization of the main M. leprae genotypes was found to be more straightforward and we observed that the SNP type 3 was most frequent, followed by SNP type 4. The allelic patterns of two minisatellite loci 27-5 and 12-5 were highly correlated with SNP type 3. Our results, besides providing additional data showing the relation between different genetic markers, suggest that VNTR analysis in M. leprae is not very different from that observed in other Mycobacterium species. The allelic index for each locus ranged between zero to 0.92. Epidemiological association between genotypes and disease form, origin or other patient characteristics was not evident in this study population and requires further investigation. Our preliminary data obtained with material extracted from Ziehl-Neelsen stained slides also suggested that this material could serve as an alternative or additional source for M. leprae genotyping. 㰀倀㸀☀渀戀猀瀀㬀㰀⼀倀㸀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀倀愀氀愀瘀爀愀猀ⴀ挀栀愀瘀攀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀䜀攀渀漀琀礀瀀椀渀最Ⰰ 䴀礀挀漀戀愀挀琀攀爀椀甀洀 氀攀瀀爀愀攀Ⰰ 嘀一吀刀Ⰰ 匀一倀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀
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