25º Congresso Brasileiro de Microbiologia

25º Congresso Brasileiro de Microbiologia

ResumoID:1048-2

Área: Microbiologia de Alimentos ( Divisão K )

OCORRÊNCIA DE LISTERIA MONOCYTOGENES EM LINHA DE PRODUÇÃO E AMBIENTE DE PROCESSAMENTO DE QUEIJO MINAS FRESCAL

Giovana Verginia Barancelli (FZEA-USP); Tarsila Mendes de Camargo (ESALQ-USP); Nicolle Ferraz de Arruda (UNIMEP); André Victor Barbosa (FIOCRUZ - RJ); Ernani Porto (ESALQ-USP); Carlos Augusto Fernandes de Oliveira (FZEA-USP); Ernesto Hofer (FIOCRUZ - RJ)

Resumo

Introdução

਀㰀倀挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀　挀洀　挀洀　瀀琀㬀䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀渀漀爀洀愀氀㬀吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀樀甀猀琀椀昀礀㬀洀猀漀ⴀ氀愀礀漀甀琀ⴀ最爀椀搀ⴀ愀氀椀最渀㨀渀漀渀攀∀㸀㰀䤀猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀渀漀爀洀愀氀∀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀㬀洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ氀愀渀最甀愀最攀㨀倀吀ⴀ䈀刀∀㸀䰀⸀ 洀漀渀漀挀礀琀漀最攀渀攀猀Ⰰ 㰀⼀匀倀䄀一㸀㰀⼀䤀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀㬀洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ氀愀渀最甀愀最攀㨀倀吀ⴀ䈀刀∀㸀挀愀甀猀愀搀漀爀愀搀愀㰀䤀猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀渀漀爀洀愀氀∀㸀㰀⼀䤀㸀氀椀猀琀攀爀椀漀猀攀栀甀洀愀渀愀Ⰰ 搀漀攀渀愀搀攀戀愀椀砀愀椀渀挀椀搀渀挀椀愀洀愀猀搀攀愀氀琀愀洀漀爀琀愀氀椀搀愀搀攀攀洀最爀甀瀀漀猀攀猀瀀攀挀昀椀挀漀猀Ⰰ  洀甀椀琀漀愀猀猀漀挀椀愀搀愀愀瀀爀漀搀甀琀漀猀氀挀琀攀漀猀Ⰰ 瀀漀爀洀㰀⼀匀倀䄀一㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀∀㸀猀甀愀漀爀椀最攀洀攀搀椀猀猀攀洀椀渀愀漀攀洀氀愀琀椀挀渀椀漀猀猀漀瀀漀甀挀漀挀漀渀栀攀挀椀搀愀猀⸀ 㰀⼀匀倀䄀一㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䌀伀䰀伀刀㨀 ⌀㈀㌀㄀昀㈀　㬀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀㬀洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ氀愀渀最甀愀最攀㨀倀吀ⴀ䈀刀∀㸀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀

਀㰀倀挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀　挀洀　挀洀　瀀琀㬀䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀渀漀爀洀愀氀㬀吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀樀甀猀琀椀昀礀㬀洀猀漀ⴀ氀愀礀漀甀琀ⴀ最爀椀搀ⴀ愀氀椀最渀㨀渀漀渀攀∀㸀㰀䈀猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀渀漀爀洀愀氀∀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀㬀洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ氀愀渀最甀愀最攀㨀倀吀ⴀ䈀刀∀㸀伀戀樀攀琀椀瘀漀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀䈀㸀㰀⼀倀㸀

Identificar os pontos de contaminação de L. monocytogenes na linha de produção do queijo Minas frescal com as caracterizações antigênica e molecular dos isolados.

਀㰀倀挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀　挀洀　挀洀　瀀琀㬀䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀渀漀爀洀愀氀㬀吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀樀甀猀琀椀昀礀㬀洀猀漀ⴀ氀愀礀漀甀琀ⴀ最爀椀搀ⴀ愀氀椀最渀㨀渀漀渀攀∀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀㬀洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ氀愀渀最甀愀最攀㨀倀吀ⴀ䈀刀∀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀洀猀漀ⴀ猀瀀愀挀攀爀甀渀㨀礀攀猀∀㸀☀渀戀猀瀀㬀㰀⼀匀倀䄀一㸀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀

Material e Métodos

਀㰀倀挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀　挀洀　挀洀㄀　瀀琀㬀䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀渀漀爀洀愀氀㬀吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀樀甀猀琀椀昀礀㬀洀猀漀ⴀ氀愀礀漀甀琀ⴀ最爀椀搀ⴀ愀氀椀最渀㨀渀漀渀攀∀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀∀㸀吀爀猀挀漀氀攀琀愀猀 ⠀琀爀椀洀攀猀琀爀愀椀猀⤀ 昀漀爀愀洀昀攀椀琀愀猀攀洀甀洀氀愀琀椀挀渀椀漀瀀爀漀搀甀琀漀爀搀攀焀甀攀椀樀漀䴀椀渀愀猀昀爀攀猀挀愀氀搀愀爀攀最椀漀搀攀倀椀爀愀挀椀挀愀戀愀ⴀ匀倀Ⰰ 㰀匀倀䄀一猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀椀琀愀氀椀挀∀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀洀猀漀ⴀ猀瀀愀挀攀爀甀渀㨀礀攀猀∀㸀☀渀戀猀瀀㬀☀渀戀猀瀀㬀㰀⼀匀倀䄀一㸀瀀愀爀愀瀀攀猀焀甀椀猀愀搀攀㰀䤀㸀䰀⸀ 洀漀渀漀挀礀琀漀最攀渀攀猀㰀⼀䤀㸀渀漀氀攀椀琀攀挀爀甀搀漀猀昀漀爀渀攀挀攀搀漀爀攀猀Ⰰ 搀漀猀猀椀氀漀猀搀攀爀攀挀攀瀀漀㰀匀倀䄀一猀琀礀氀攀㴀∀洀猀漀ⴀ猀瀀愀挀攀爀甀渀㨀礀攀猀∀㸀☀渀戀猀瀀㬀㰀⼀匀倀䄀一㸀攀渀漀攀猀琀愀戀攀氀攀挀椀洀攀渀琀漀㨀瀀椀猀漀猀Ⰰ 爀愀氀漀猀Ⰰ 攀焀甀椀瀀愀洀攀渀琀漀猀攀甀琀攀渀猀氀椀漀猀Ⰰ 最甀愀Ⰰ 氀攀椀琀攀瀀愀猀琀攀甀爀椀稀愀搀漀Ⰰ 猀愀氀洀漀甀爀愀攀焀甀攀椀樀漀⸀ 㰀⼀匀倀䄀一㸀䄀猀愀渀氀椀猀攀猀昀漀爀愀洀昀攀椀琀愀猀挀漀渀昀漀爀洀攀漀䘀䐀䄀 ⠀䠀椀琀挀栀椀渀猀Ⰰ ㈀　　㌀⤀㰀䤀㸀㰀⼀䤀㸀挀漀洀攀渀爀椀焀甀攀挀椀洀攀渀琀漀攀洀䈀䰀䔀䈀攀瀀氀愀焀甀攀愀洀攀渀琀漀攀洀洀攀椀漀猀伀砀昀漀爀搀攀䄀䰀伀䄀Ⰰ 挀漀渀昀椀爀洀愀漀瀀攀氀漀䬀椀琀䄀瀀椀㰀䤀㸀䰀椀猀琀攀爀椀愀㰀⼀䤀㸀攀愀猀漀爀漀氀漀最椀愀搀愀猀挀攀瀀愀猀搀攀㰀䤀猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀渀漀爀洀愀氀∀㸀䰀⸀ 洀漀渀漀挀礀琀漀最攀渀攀猀㰀⼀䤀㸀昀漀椀爀攀愀氀椀稀愀搀愀㰀⼀匀倀䄀一㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀㬀洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ氀愀渀最甀愀最攀㨀倀吀ⴀ䈀刀㬀洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ昀漀渀琀ⴀ昀愀洀椀氀礀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀∀㸀瀀攀氀攀琀挀渀椀挀愀搀愀愀最氀甀琀椀渀愀漀爀瀀椀搀愀Ⰰ 甀琀椀氀椀稀愀渀搀漀愀渀琀椀猀猀漀爀漀猀猀漀洀琀椀挀漀猀攀昀氀愀最攀氀愀爀攀猀瀀漀氀椀瘀愀氀攀渀琀攀猀攀洀漀渀漀瘀愀氀攀渀琀攀猀 ⠀匀攀攀氀椀最攀爀 ☀愀洀瀀㬀䠀栀渀攀Ⰰ ㄀㤀㜀㤀⤀⸀㰀⼀匀倀䄀一㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀∀㸀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀

Resultados e Discussão

਀㰀倀挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀　挀洀　挀洀㄀　瀀琀㬀䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀渀漀爀洀愀氀㬀吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀樀甀猀琀椀昀礀㬀洀猀漀ⴀ氀愀礀漀甀琀ⴀ最爀椀搀ⴀ愀氀椀最渀㨀渀漀渀攀∀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀∀㸀䄀琀 漀洀漀洀攀渀琀漀Ⰰ 㰀䤀㸀䰀⸀ 洀漀渀漀挀礀琀漀最攀渀攀猀㰀⼀䤀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀椀琀愀氀椀挀∀㸀渀漀昀漀椀椀猀漀氀愀搀愀㰀⼀匀倀䄀一㸀搀漀氀攀椀琀攀挀爀甀搀愀猀昀愀稀攀渀搀愀猀 ⠀一㴀㜀　⤀Ⰰ 搀漀猀猀椀氀漀猀搀漀猀氀愀琀椀挀渀椀漀猀 ⠀一㴀㌀⤀Ⰰ 搀漀氀攀椀琀攀瀀愀猀琀攀甀爀椀稀愀搀漀 ⠀一㴀㌀⤀Ⰰ 搀愀 最甀愀 ⠀一㴀㌀⤀Ⰰ 搀漀挀漀最甀氀漀 ⠀一㴀㌀⤀ 攀搀漀猀焀甀攀椀樀漀猀攀洀瀀爀漀挀攀猀猀愀洀攀渀琀漀 ⠀一㴀㄀㠀⤀ 漀甀攀洀戀愀氀愀搀漀猀 ⠀一㴀㄀㔀⤀⸀ 䄀洀漀猀琀爀愀猀瀀漀猀椀琀椀瘀愀猀昀漀爀愀洀攀渀挀漀渀琀爀愀搀愀猀攀洀猀愀氀洀漀甀爀愀 ⠀㌀㌀Ⰰ㐀─Ⰰ 一㴀㌀⤀ 攀攀洀洀漀猀攀渀氀甀瘀愀搀愀猀搀攀洀愀渀椀瀀甀氀愀搀漀爀⸀ 一漀琀漀琀愀氀Ⰰ 昀漀爀愀洀愀渀愀氀椀猀愀搀愀猀㤀㘀愀洀漀猀琀爀愀猀搀愀椀渀搀切猀琀爀椀愀Ⰰ 猀攀渀搀漀㐀㠀搀攀愀氀椀洀攀渀琀漀猀Ⰰ 最甀愀攀猀愀氀洀漀甀爀愀攀㐀㠀愀洀戀椀攀渀琀愀椀猀⸀ 㰀匀倀䄀一猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ眀攀椀最栀琀㨀戀漀氀搀∀㸀䐀愀猀愀洀漀猀琀爀愀猀愀洀戀椀攀渀琀愀椀猀愀渀愀氀椀猀愀搀愀猀愀琀 漀洀漀洀攀渀琀漀Ⰰ 㰀䤀猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀渀漀爀洀愀氀∀㸀䰀⸀ 洀漀渀漀挀礀琀漀最攀渀攀猀㰀⼀䤀㸀昀漀椀椀猀漀氀愀搀愀搀攀㠀Ⰰ㌀─Ⰰ 猀攀渀搀漀㄀㘀Ⰰ㜀─ ⠀一㴀㄀㠀⤀ 搀攀猀甀瀀攀爀昀挀椀攀猀猀攀洀挀漀渀琀愀琀漀挀漀洀焀甀攀椀樀漀猀攀㌀Ⰰ㌀─ ⠀一㴀㌀　⤀ 搀攀猀甀瀀攀爀昀挀椀攀猀挀漀洀挀漀渀琀愀琀漀挀漀洀焀甀攀椀樀漀⸀㰀匀倀䄀一猀琀礀氀攀㴀∀洀猀漀ⴀ猀瀀愀挀攀爀甀渀㨀礀攀猀∀㸀☀渀戀猀瀀㬀㰀⼀匀倀䄀一㸀㰀䤀猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀渀漀爀洀愀氀∀㸀䰀⸀ 椀渀渀漀挀甀愀㰀⼀䤀㸀昀漀椀瀀漀猀椀琀椀瘀愀攀洀㄀㔀Ⰰ㘀─ ⠀一㴀㤀㘀⤀Ⰰ 猀攀渀搀漀㈀㜀Ⰰ㄀─ ⠀一㴀㐀㠀⤀ 攀洀愀洀漀猀琀爀愀猀愀洀戀椀攀渀琀愀椀猀搀攀猀猀愀猀㘀㘀Ⰰ㜀─ 攀洀猀甀瀀攀爀昀挀椀攀猀猀攀洀挀漀渀琀愀琀漀挀漀洀焀甀攀椀樀漀猀攀㌀Ⰰ㌀㌀─ 搀攀猀甀瀀攀爀昀挀椀攀猀挀漀洀挀漀渀琀愀琀漀⸀ 伀猀椀猀漀氀愀搀漀猀搀攀㰀䤀猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀渀漀爀洀愀氀∀㸀䰀⸀ 洀漀渀漀挀礀琀漀最攀渀攀猀㰀⼀䤀㸀猀甀戀洀攀琀椀搀漀猀  猀漀爀漀氀漀最椀愀爀攀瘀攀氀愀爀愀洀ⴀ猀攀搀攀琀爀猀猀漀爀漀琀椀瀀漀猀㨀㰀⼀匀倀䄀一㸀㐀戀 ⠀㜀㈀Ⰰ㜀─⤀Ⰰ ㄀⼀㈀挀 ⠀㄀㠀Ⰰ㈀─⤀ 攀㄀⼀㈀愀 ⠀㤀Ⰰ㄀─⤀⸀ 䄀瀀猀漀琀爀洀椀渀漀搀愀猀挀漀氀攀琀愀猀Ⰰ 猀攀爀 昀攀椀琀愀愀挀愀爀愀挀琀攀爀椀稀愀漀洀漀氀攀挀甀氀愀爀搀漀猀椀猀漀氀愀搀漀猀搀攀㰀䤀㸀䰀⸀ 洀漀渀漀挀礀琀漀最攀渀攀猀㰀⼀䤀㸀Ⰰ 瀀漀爀倀䘀䜀䔀⸀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀

Conclusões

਀㰀倀挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀　挀洀　挀洀　瀀琀㬀䰀䤀一䔀ⴀ䠀䔀䤀䜀䠀吀㨀渀漀爀洀愀氀㬀吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀樀甀猀琀椀昀礀㬀洀猀漀ⴀ氀愀礀漀甀琀ⴀ最爀椀搀ⴀ愀氀椀最渀㨀渀漀渀攀∀㸀㰀匀倀䄀一猀琀礀氀攀㴀∀䘀伀一吀ⴀ䘀䄀䴀䤀䰀夀㨀 ✀吀椀洀攀猀一攀眀刀漀洀愀渀✀Ⰰ✀猀攀爀椀昀✀∀㸀䄀瀀爀攀猀攀渀愀㰀䤀猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀渀漀爀洀愀氀∀㸀䰀⸀ 洀漀渀漀挀礀琀漀最攀渀攀猀㰀⼀䤀㸀渀漀愀洀戀椀攀渀琀攀搀攀瀀爀漀搀甀漀攀瀀爀漀挀攀猀猀愀洀攀渀琀漀搀攀焀甀攀椀樀漀猀  昀漀渀琀攀瀀漀琀攀渀挀椀愀氀搀攀挀漀渀琀愀洀椀渀愀漀攀栀漀甀瘀攀瀀爀攀搀漀洀渀椀漀搀漀猀漀爀漀琀椀瀀漀㐀戀⸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀

Palavras-chave: Listeria monocytogenes; Laticínio; Queijo minas frescal; Caracterização molecular; Caracterização antigência.

Palavras-chave: Caracterização antigência, Caracterização molecular, Laticínio, Listeria monocytogenes, Queijo minas frescal

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