ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:743-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Ecologia Microbiana ( Divisão I )</b><p align=justify><strong><FONT SIZE=4 FACE="TIMES NEW ROMAN, TIMES, SERIF"><STRONG>YEASTS ISOLATED FROM LOCAL HONEY SAMPLES OBTAINED FROM THE STATE OF MATO GROSSO DO SUL</STRONG></FONT>&NBSP;</strong></p><p align=justify><b><u>Ifeloju Dayo-owoyemi </u></b> (<i>UNESP</i>); <b>Andre Rodrigues </b> (<i>UNESP</i>); <b>Osmar Malaspina </b> (<i>UNESP</i>); <b>Vladson Carbonari </b> (<i>UNESP</i>); <b>Fernando Carlos Pagnocca </b> (<i>UNESP</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2><P style="TEXT-ALIGN: justify; MARGIN: 0cm 0cm 0pt" class=MsoNormal><SPAN style="mso-ansi-language: EN-US" lang=EN-US>In the course of a study to identify yeasts present in local honey samples obtained from <EM>Apis mellifera</EM> of the state of Mato Grosso do Sul, 13 yeast strains were isolated from 13 honey samples screened. Overall, grouping based on morphological and physiological characteristics as well as microsatellite (MSP-PCR) fingerprints yielded 8 distinct groups represented by strains MP2-2CA (n= 1 isolate), MP2-2CB (n= 1), MP7-7B (n= 1), MP8-8B (n= 2), MP9-9A (n= 4), MP12-12A (n= 2), MP14-14A (n= 1) and MP14-14B (n= 1). Various cell shapes ranging from oval, ellipsoidal, spherical and cylindrical were observed on the yeast isolates. Strains represented by MP12-12A and MP8-8B assimilated galactose and formed ascospores after 3 days of incubation in glucose yeast peptone broth. Strain MP2-2CA showed a similar fingerprint profile with yeasts in groups MP7-7B and MP9-9A, but differed by assimilating maltose and xilitol. Strain MP2-2CB assimilated galactose, maltose, lactose, D-xilose, D-arabinose and L-arabinose. Based on&nbsp;sequences of D1/D2 domains of the rDNA gene 11 isolates were identified as <I style="mso-bidi-font-style: normal">Zygosaccharomyces mellis</I> (groups represented by MP2-2CA, MP7-7B, MP8-8B, MP9-9A, MP12-12A and MP14-14A) with nucleotide identities ranging from 97-100% according to NCBI-BLASTn. One strain (MP2-2CB) showed 100% sequence identity with <I style="mso-bidi-font-style: normal">Pseudozyma hubeiensis</I> and strain MP14-14B was identified as <I style="mso-bidi-font-style: normal">Zygosaccharomyces</I> sp. Physicochemical analysis of the honey samples showed that their concentration of reducing sugars ranged between 65.10%&nbsp;- 70.22%, with sucrose, glucose and fructose concentrations ranging between 0.69% - 2.28%, 29.62% - 31.62% and 35.79% - 38.77%, respectively. The present work revealed that the honey samples investigated presented few yeast species recovered by&nbsp;culture techniques. It is likely that the high sugar concentrations&nbsp;permitted the growth of osmotolerant yeasts, like <I style="mso-bidi-font-style: normal">Z. mellis</I>. All strains recovered grew on 40% glucose medium but weakly in medium containing 0.5% glucose suggesting that these strains are likely adapted to high sugar concentrations. </SPAN></P> <P style="TEXT-ALIGN: justify; MARGIN: 0cm 0cm 0pt" class=MsoNormal><SPAN style="mso-ansi-language: EN-US" lang=EN-US><?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></SPAN>&nbsp;</P> <P style="TEXT-ALIGN: justify; MARGIN: 0cm 0cm 0pt" class=MsoNormal><SPAN style="mso-ansi-language: EN-US" lang=EN-US>Acknowledgment: TWAS/CNPq for the scholarship provided to I. </SPAN><SPAN style="mso-ansi-language: EN-GB" lang=EN-GB>Dayo-Owoyemi</SPAN><SPAN style="mso-ansi-language: EN-US" lang=EN-US><o:p></o:p></SPAN></P></font></p><br><b>Palavras-chave: </b>&nbsp;Zygosaccharomyces, osmotolerant, Apis</td></tr></table></tr></td></table></body></html>