ANALYSIS OF CARBON SOURCE AND PH-DEPENDENT TRANSCRIPTIONAL REGULATION OF HUMICOLA GRISEA VAR. THERMOIDEA LIGNOCELLULOLYTIC SYSTEM਀ ਀ ਀㰀⼀猀琀爀漀渀最㸀㰀⼀瀀㸀㰀瀀 愀氀椀最渀㴀樀甀猀琀椀昀礀㸀㰀戀㸀㰀甀㸀吀栀椀愀最漀 䴀愀挀栀愀搀漀 䴀攀氀氀漀 搀攀 匀漀甀猀愀 㰀⼀甀㸀㰀⼀戀㸀  ⠀㰀椀㸀唀渀䈀㰀⼀椀㸀⤀㬀 㰀戀㸀䰀漀爀攀渀愀  搀愀 匀椀氀瘀攀椀爀愀 䐀攀爀攀渀最漀眀椀猀欀椀 㰀⼀戀㸀  ⠀㰀椀㸀唀渀䈀㰀⼀椀㸀⤀㬀 㰀戀㸀䤀氀搀椀渀攀琀攀  匀椀氀瘀愀 倀攀爀攀椀爀愀 㰀⼀戀㸀  ⠀㰀椀㸀唀渀䈀㰀⼀椀㸀⤀㬀 㰀戀㸀䴀愀爀挀椀漀  䨀漀猀 倀漀愀猀 䘀漀渀猀攀挀愀 㰀⼀戀㸀  ⠀㰀椀㸀唀渀䈀㰀⼀椀㸀⤀㰀戀爀㸀㰀戀爀㸀㰀⼀瀀㸀㰀戀㸀㰀昀漀渀琀 猀椀稀攀㴀㈀㸀刀攀猀甀洀漀㰀⼀昀漀渀琀㸀㰀⼀戀㸀㰀瀀 愀氀椀最渀㴀樀甀猀琀椀昀礀 挀氀愀猀猀㴀琀爀攀猀㸀㰀昀漀渀琀 猀椀稀攀㴀㈀㸀䔀渀瘀椀爀漀渀洀攀渀琀愀氀 pH is an important signal for fungi physiology, intervening at the਀琀爀愀渀猀挀爀椀瀀琀椀漀渀愀氀 爀攀最甀氀愀琀椀漀渀 漀昀 猀攀瘀攀爀愀氀 最攀渀攀 瀀爀漀搀甀挀琀猀⸀ 䤀渀 昀椀氀愀洀攀渀琀漀甀猀 fungi and yeasts, the PacC zinc-finger transcription factor regulates਀最攀渀攀 攀砀瀀爀攀猀猀椀漀渀 椀渀 爀攀猀瀀漀渀猀攀 琀漀 愀氀欀愀氀椀渀攀 攀砀琀攀爀渀愀氀 瀀䠀⸀ The਀瀀爀漀搀甀挀琀椀漀渀 漀昀 攀渀稀礀洀攀猀 椀渀瘀漀氀瘀攀搀 椀渀 瀀氀愀渀琀 挀攀氀氀 眀愀氀氀 戀爀攀愀欀搀漀眀渀 椀猀 regulated mainly at the transcriptional level. Nonetheless, the਀椀渀瘀漀氀瘀攀洀攀渀琀 漀昀 琀栀攀 瀀䠀ⴀ爀攀氀愀琀攀搀 爀攀最甀氀愀琀漀爀礀 瀀愀琀栀眀愀礀 椀渀 琀栀攀 lignocellulolytic enzymes expression has not been extensively਀猀琀甀搀椀攀搀⸀ 圀攀 栀愀瘀攀 搀攀洀漀渀猀琀爀愀琀攀搀 琀栀愀琀 琀栀攀 琀栀攀爀洀漀瀀栀椀氀椀挀 搀攀甀琀攀爀漀洀礀挀攀琀攀 Humicola਀最爀椀猀攀愀 var. thermoidea਀椀猀 a potent cellulases producer, presenting a considerable potential for਀愀最爀椀挀甀氀琀甀爀愀氀 眀愀猀琀攀猀 戀椀漀挀漀渀瘀攀爀琀椀漀渀 瀀爀漀挀攀猀猀攀猀⸀ 刀攀猀甀氀琀猀 漀昀 漀甀爀 最爀漀甀瀀 support the existence of a pH regulatory pathway for H.਀最爀椀猀攀愀 var. thermoidea਀琀爀愀渀猀挀爀椀瀀琀椀漀渀愀氀 爀攀最甀氀愀琀椀漀渀⸀ 䤀渀 琀栀椀猀 眀漀爀欀Ⰰ 眀攀 栀愀瘀攀 瀀攀爀昀漀爀洀攀搀 戀礀 quantitative real time RT-PCR a time course trascriptional analysis਀漀昀 猀攀瘀攀爀愀氀 䠀⸀ grisea਀ 最攀渀攀猀⸀ 䔀椀最栀琀 氀椀最渀漀挀攀氀氀甀氀漀氀礀琀椀挀 最攀渀攀猀 ⠀挀戀栀㄀⸀㄀Ⰰ cbh1.2,਀攀最氀㄀Ⰰ egl2,਀攀最氀㌀Ⰰ egl4,਀戀最氀㐀 and xyn1)਀愀渀搀 two transcription factors (pacC਀愀渀搀 挀爀攀䄀⤀ were਀愀渀愀氀礀稀攀搀 椀渀 the presence of simple (glucose) or complex (sugarcane bagasse)਀挀愀爀戀漀渀 猀漀甀爀挀攀猀 愀渀搀 椀渀 愀挀椀搀 漀爀 愀氀欀愀氀椀渀攀 洀攀搀椀甀洀 挀漀渀搀椀琀椀漀渀猀⸀ 吀栀攀 焀刀吀ⴀ倀䌀刀 analysis reveled an early and strong induction of transcription of਀愀氀洀漀猀琀 愀氀氀 氀椀最渀漀挀攀氀氀甀氀漀氀椀琀挀 最攀渀攀猀Ⰰ 椀渀 愀 猀礀渀攀爀最椀猀琀椀挀 眀愀礀Ⰰ 眀栀攀渀 琀栀攀 mycelia were grown with the complex carbon source and in alkali਀挀漀渀搀椀琀椀漀渀猀 ⠀瀀䠀 㠀⸀　⤀⸀ 吀栀攀 漀渀氀礀 攀砀挀攀瀀琀椀漀渀 眀愀猀 攀最氀㐀Ⰰ that਀眀愀猀 愀挀椀搀 椀渀搀甀挀攀搀⸀ 䄀渀 opposite pattern of expression of the two trascription factors was਀漀戀猀攀爀瘀攀搀⸀ 圀栀椀氀攀 瀀愀挀䌀 was induced in alkaline conditions and strongly repressed in presence਀漀昀 最氀甀挀漀猀攀Ⰰ 挀爀攀䄀 was induced by glucose and repressed in alkaline conditions. By਀攀氀攀琀爀漀瀀栀漀爀攀琀椀挀 洀漀戀椀氀椀琀礀 猀栀椀昀琀 愀猀猀愀礀猀 ⠀䔀䴀匀䄀猀⤀ 眀椀琀栀 甀瀀猀琀爀攀愀洀 爀攀最甀氀愀琀漀爀礀 sequences of pacC,਀眀攀 showed਀琀栀愀琀 攀砀椀猀琀猀 愀渀 in਀瘀椀琀爀漀 interaction between the proteins PacC and CreA with pacC਀甀瀀猀琀爀攀愀洀 爀攀最甀氀愀琀漀爀礀 猀攀焀甀攀渀挀攀Ⰰ 眀栀攀爀攀 琀栀攀 戀漀琀栀 昀愀挀琀漀爀 挀漀洀瀀攀琀攀 戀礀 琀栀攀 same binding site.਀吀愀欀攀渀 together, this data corroborates our previous evidences supporting਀琀栀攀 攀砀椀猀琀攀渀挀攀 漀昀 愀 瀀䠀 爀攀最甀氀愀琀漀爀礀 瀀愀琀栀眀愀礀 昀漀爀 䠀⸀ grisea਀琀爀愀渀猀挀爀椀瀀琀椀漀渀愀氀 爀攀最甀氀愀琀椀漀渀 戀礀 倀愀挀䌀⸀ 䴀漀爀攀漀瘀攀爀Ⰰ 倀愀挀䌀 椀猀 瀀爀漀戀愀戀氀礀 transcriptionaly regulated by itself and may suffer influence of the਀挀愀爀戀漀渀 repression mechanism mediated by CreA.਀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀倀愀氀愀瘀爀愀猀ⴀ挀栀愀瘀攀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀瀀䠀Ⰰ 琀爀愀渀猀挀爀椀瀀琀椀漀渀愀氀 爀攀最甀氀愀琀椀漀渀Ⰰ 倀愀挀䌀Ⰰ 䌀爀攀䄀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀