25º Congresso Brasileiro de Microbiologia

25º Congresso Brasileiro de Microbiologia

ResumoID:630-1

Área: Micologia Médica ( Divisão B )

ACTION OF ORGANOSULFUR RUTHENIUM AS INHIBITOR OF CANDIDA SPP. ADHESION TO HUMAN BUCCAL EPITHELIAL CELLS

Luciano José Nogueira (UFMG); Claudio Luis Donnici (UFMG); Thaís Furtado Ferreira Magalhães (UFMG); Maria Aparecida de Resende (UFMG); Cleide Viviane Buzanello Martins (UFMG)

Resumo

Various in vitro studies and animal studies have provided evidence for a relationship between the proclivity of opportunistic Candida species to adhere to mucosal surfaces (vascular structures such as endothelial cells and subendothelial basement membrane) and their presence in infections. Therefore candidal adherence to human buccal epithelial cells (BECs) is considered as the critical initial step in the pathogenesis of oral candidiasis, which may lead to eventual systemic infection, especially in compromised people. Facing of candidiasis increased, the discovery of new antifungal agents thus remains an important challenge for the scientific community and metal-based drugs might represent an alternative therapeutic route.

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The adhesion testing was performed in accordance with method described by literature and it was tested against four Candida species (C. albicans, C. parapsilosis, C. tropicalis and two clinical isolates strains of C. dubliniensis). Fluconazole (FLC) was used as control. The effect of antifungal drugs on candidal adhesion to BECs has been examined following limited exposure (1h) of the isolates to sublethal concentration of the drug. The preparations of BEC were air dried, fixed with heat and stained with gentian violet. The number of adherent yeast cells was quantified by light microscopy at 400 magnification. In each experiment, 50 BECs were observed for adherent yeast cells. Clumped, folded, or overlapping BECs were excluded.

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In conclusion, inhibition of Candida spp. adhesion by ruthenium dithiocarbamate complexes is clearly epithelial cell-specific for preponderance of strains. Therefore, the presence of drug during the "adherence phase" may be of clinical relevance. These results addition others publications (Minimum Inhibitory Concentration from 10^–6-10^–8 micromol.mL^–1 against some important fungal pathogens) proved the possible application of these ruthenium complexes as new drugs for invasive fungal infections .

Palavras-chave: ADESÃO, CANDIDA, MIC

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