| Área: Micobacteriologa ( Divisão C ) KINETIC AND BIOCHEMICAL CHARACTERIZATION OF RECOMBINAT ENZIME ADENYLOSUCCINATE SINTHASE (EC 6.3.4.4) FROM MYCOBACTERIUM TUBERCULOSIS
Léia Jaskulski (INCTTB); Luiz Augusto Basso (INCTTB); Diógenes Santiago Santos (INCTTB)
ResumoMycobacterium tuberculosis, the causative agent of tuberculosis (TB), is a pathogenic bacterium that currently infects one-third of the world's population. Although there are effective drugs for TB treatment, the disease is not properly controlled in many poor and developing countries. Drugs that can act against strains of M. tuberculosis would contribute to effective control of this disease. A recent approach for developing new anti-TB agents is rational drug design. Therefore, studies of possible new molecular microbial targets are fundamental. 㰀倀 挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀 猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀 椀渀 椀渀 瀀琀㬀 吀䔀堀吀ⴀ䤀一䐀䔀一吀㨀 ㌀㔀⸀㐀㔀瀀琀㬀 吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀 樀甀猀琀椀昀礀∀㸀㰀匀倀䄀一 猀琀礀氀攀㴀∀洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀㬀 洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ昀愀洀椀氀礀㨀 䄀爀椀愀氀㬀 洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀䔀渀稀礀洀攀猀 漀昀 瀀甀爀椀渀攀 戀椀漀猀礀渀琀栀攀猀椀猀 椀渀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䴀⸀ 琀甀戀攀爀挀甀氀漀猀椀猀㰀⼀䤀㸀Ⰰ 眀栀椀挀栀 愀爀攀 爀攀焀甀椀爀攀搀 昀漀爀 洀礀挀漀戀愀挀琀攀爀椀愀氀 最爀漀眀琀栀Ⰰ 栀愀瘀攀 戀攀攀渀 攀砀琀攀渀猀椀瘀攀氀礀 猀琀甀搀椀攀搀 戀礀 漀甀爀 爀攀猀攀愀爀挀栀 最爀漀甀瀀Ⰰ 瀀爀漀瘀椀搀椀渀最 洀愀渀礀 渀漀瘀攀氀 琀愀爀最攀琀猀 昀漀爀 爀愀琀椀漀渀愀氀 搀攀猀椀最渀 漀昀 洀漀爀攀 攀昀昀攀挀琀椀瘀攀 愀渀琀椀洀礀挀漀戀愀挀琀攀爀椀愀氀 愀最攀渀琀猀⸀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀
Adenylosuccinate synthase governs the committed step in the de novo biosynthesis of AMP, coupling the hydrolysis of GTP with the synthesis of adenylosuccinate from L-aspartate and IMP. Because this enzyme acts on the synthesis of purine nucleotides at a critical step, it can be essential for mycobacterial growth. Accordingly, structural and functional studies should be undertaken in order to explore differences in the mode of action and relevant features for this M. tuberculosis enzyme. 㰀倀 挀氀愀猀猀㴀䴀猀漀一漀爀洀愀氀 猀琀礀氀攀㴀∀䴀䄀刀䜀䤀一㨀 椀渀 椀渀 瀀琀㬀 吀䔀堀吀ⴀ䤀一䐀䔀一吀㨀 ㌀㔀⸀㐀㔀瀀琀㬀 吀䔀堀吀ⴀ䄀䰀䤀䜀一㨀 樀甀猀琀椀昀礀∀㸀㰀匀倀䄀一 猀琀礀氀攀㴀∀洀猀漀ⴀ昀愀爀攀愀猀琀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀㬀 洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ昀愀洀椀氀礀㨀 䄀爀椀愀氀㬀 洀猀漀ⴀ愀渀猀椀ⴀ氀愀渀最甀愀最攀㨀 䔀一ⴀ唀匀∀㸀䐀一䄀 猀攀焀甀攀渀挀攀 漀昀 䄀搀攀渀礀氀漀猀甀挀挀椀渀愀琀攀 匀礀渀琀栀愀猀攀 ⠀䔀䌀 㘀⸀㌀⸀㐀⸀㐀⤀ 昀爀漀洀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䴀⸀ 琀甀戀攀爀挀甀氀漀猀椀猀㰀⼀䤀㸀 䠀㌀㜀刀瘀 眀愀猀 漀戀琀愀椀渀攀搀 昀爀漀洀 瀀甀戀氀椀挀 搀愀琀愀戀愀猀攀猀⸀ 吀栀攀 挀漀爀爀攀猀瀀漀渀搀椀渀最 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀瀀甀爀䄀㰀⼀䤀㸀 挀漀搀椀渀最 猀攀焀甀攀渀挀攀 眀愀猀 愀洀瀀氀椀昀椀攀搀 昀爀漀洀 最攀渀漀洀椀挀 䐀一䄀 甀猀椀渀最 猀瀀攀挀椀昀椀挀 瀀爀椀洀攀爀猀 愀渀搀 倀䌀刀 猀琀愀渀搀愀爀搀 挀漀渀搀椀琀椀漀渀猀⸀ 吀栀攀 漀戀琀愀椀渀攀搀 昀爀愀最洀攀渀琀 眀愀猀 挀氀漀渀攀搀 椀渀琀漀 琀栀攀 瀀䌀刀ⴀ䈀氀甀渀琀 瘀攀挀琀漀爀 ⠀䤀渀瘀椀琀爀漀最攀渀⤀ 愀渀搀 猀甀戀挀氀漀渀攀搀 椀渀琀漀 琀栀攀 瀀䔀吀ⴀ㈀㌀愀⠀⬀⤀ 攀砀瀀爀攀猀猀椀漀渀 瘀攀挀琀漀爀 ⠀一漀瘀愀最攀渀⤀⸀ 一甀挀氀攀漀琀椀搀攀 猀攀焀甀攀渀挀攀 漀昀 琀栀攀 挀氀漀渀攀搀 昀爀愀最洀攀渀琀 眀愀猀 搀攀琀攀爀洀椀渀攀搀 戀礀 愀甀琀漀洀愀琀攀搀 䐀一䄀 猀攀焀甀攀渀挀椀渀最⸀ 䐀椀昀昀攀爀攀渀琀 猀琀爀愀椀渀猀 漀昀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䔀猀挀栀攀爀椀挀栀椀愀 挀漀氀椀㰀⼀䤀㸀 眀攀爀攀 琀攀猀琀攀搀 昀漀爀 攀砀瀀爀攀猀猀椀漀渀 漀昀 琀栀攀 爀攀挀漀洀戀椀渀愀渀琀 攀渀稀礀洀攀⸀ 吀栀攀 攀渀稀礀洀攀 眀愀猀 攀砀瀀爀攀猀猀攀搀 椀渀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䔀⸀ 挀漀氀椀㰀⼀䤀㸀 䈀䰀㈀ ⠀䐀䔀㌀⤀ 挀攀氀氀猀 椀渀 琀栀攀 椀渀猀漀氀甀戀氀攀 昀爀愀挀琀椀漀渀Ⰰ 愀渀搀 猀漀氀甀戀椀氀椀稀愀琀椀漀渀 眀愀猀 愀挀栀椀攀瘀攀搀 甀猀椀渀最 愀 昀爀攀攀稀攀ⴀ琀栀愀眀 瀀爀漀琀漀挀漀氀⸀ 伀瀀琀椀洀椀稀愀琀椀漀渀 漀昀 瀀爀漀琀攀椀渀 攀砀瀀爀攀猀猀椀漀渀 椀渀 漀琀栀攀爀 㰀䤀 猀琀礀氀攀㴀∀洀猀漀ⴀ戀椀搀椀ⴀ昀漀渀琀ⴀ猀琀礀氀攀㨀 渀漀爀洀愀氀∀㸀䔀⸀ 挀漀氀椀㰀⼀䤀㸀 猀琀爀愀椀渀猀 愀渀搀 攀渀稀礀洀攀 猀漀氀甀戀椀氀椀稀愀琀椀漀渀 瀀爀漀琀漀挀漀氀猀 愀爀攀 挀甀爀爀攀渀琀氀礀 戀攀椀渀最 瀀攀爀昀漀爀洀攀搀 琀漀 瀀爀漀瘀椀搀攀 洀愀琀攀爀椀愀氀 攀渀漀甀最栀 昀漀爀 瀀甀爀椀昀椀挀愀琀椀漀渀 漀昀 琀栀攀 爀攀挀漀洀戀椀渀愀渀琀 瀀爀漀琀攀椀渀⸀ 䘀甀爀琀栀攀爀 挀栀愀爀愀挀琀攀爀椀稀愀琀椀漀渀 漀昀 琀栀攀 攀渀稀礀洀攀 琀栀爀攀攀ⴀ搀椀洀攀渀猀椀漀渀愀氀 猀琀爀甀挀琀甀爀攀 愀渀搀 猀琀甀搀礀 漀昀 椀琀猀 攀渀稀礀洀漀氀漀最椀挀愀氀 瀀爀漀瀀攀爀琀椀攀猀 眀椀氀氀 戀攀 搀攀琀攀爀洀椀渀攀搀 氀愀琀攀爀⸀㰀漀㨀瀀㸀㰀⼀漀㨀瀀㸀㰀⼀匀倀䄀一㸀㰀⼀倀㸀
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