ÿþ<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>ResumoID:571-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Virologia ( Divisão P )</b><p align=justify><strong><P><FONT SIZE="3"><SPAN STYLE="FONT-FAMILY: ARIAL,HELVETICA,SANS-SERIF;">VIRAL MENINGITIS ASSOCIATED WITH ECHOVIRUS 11 IN SÃO PAULO STATE, BRAZIL </SPAN></FONT><BR></P> </strong></p><p align=justify><b><u>Bráulio Caetano Machado </u></b> (<i>IAL</i>); <b>Denise Hage Russo </b> (<i>IAL</i>); <b>Adriana Luchs </b> (<i>IAL</i>); <b>Telma Regina Carvalhanas </b> (<i>CVE</i>); <b>Rita de Cássia Carmona </b> (<i>IAL</i>); <b>Maria do Carmo Timenetsky </b> (<i>IAL</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2> <style type="text/css"> <!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --></style><style type="text/css"> <!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --> </style> <p class="western" style="margin-bottom: 0cm; line-height: 200%; text-align: justify; font-family: Arial,Helvetica,sans-serif;"> <font size="3"><span lang="en-US"><span style="">Echovirus 11(E11) is reported among the most commonly isolated in the </span></span></font><font size="3"><span lang="en-US"><i><span style="">Human Enterovirus </span></i></span></font><font size="3"><span lang="en-US"><span style=""> (HEV) genus. E11 may cause the full range of </span></span></font><font size="3"><span lang="en-US"><i><span style="">Enterovirus </span></i></span></font><font size="3"><span lang="en-US"><span style="">diseases, but it has been particularly associated with cases of severe disease in neonates. According with literature data E11 serotype is one of the most frequent HEV isolated in USA and Spain. In Brazil there is no relates of usual circulation of E11 in viral meningitis cases. This work has the aim to relate E11 outbreaks in two different regions of São Paulo State originating meningitis in 2008. The Enteric Virus laboratory plays an important role with the diagnostic routine for viral meningitis outbreaks in the State collaborating with the </span></span></font><font size="3"><span lang="en-US"><span style="">Epidemiological Surveillance Center (CVE) for elaboration of public health measures decisions and establishing new conducts for viral containment. The Laboratory received samples of 25 suspected cases from two country cities, of São Paulo State </span></span></font><font size="3"><span lang="en-US"><span style="">located in the North and the West regions</span></span></font><font size="3"><span lang="en-US"><span style="">. The methodologies applied for the laboratory diagnosis were viral isolation using two different cell lineages RD (Human Rabdomyosarcoma) and HEp-2 (Human larynx carcinoma), RT-PCR (Reverse Transcription  Polymerase Chain Reaction) with primers directed to the conserved region of HEV genome (5'NTR), and IFA (Indirect Immunofluorescense Assay). Antibody titration in paired sera samples were also realized for the serological diagnosis. The results obtained confirmed the echovirus 11 serotype circulating in two different moments (February, March and August,September months) in distinct regions of the State. Our study documents the E11 circulation and contribute with further epidemiological investigations of HEV prevalence in São Paulo State </span></span></font><font size="3"><span lang="en-US">to provide a better understanding of virus transmission and evolution</span></font><font size="3"><span lang="en-US"><span style="">.</span></span></font></p> <style type="text/css"> <!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --> </style> <style type="text/css"> <!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --></style><style type="text/css"> <!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --> </style> </font></p><br><b>Palavras-chave: </b>&nbsp;echovirus 11, Human Enterovirus, Viral Meningitis, RT-PCR, IFA</td></tr></table></tr></td></table></body></html>