25º Congresso Brasileiro de Microbiologia

25º Congresso Brasileiro de Microbiologia

ResumoID:239-2

Área: Microbiologia de Alimentos ( Divisão K )

MICROBIOLOGICAL ANALYSIS OF MUSSELS PERNA PERNA AND MYTELLA SP. IN NATURE AND PRE-COOKED, SOLD IN THE CITIES&NBSP; OF VITÓRIA AND VILA VELHA, ES.

Tâmara de Almeida Jaretta (UVV); Thaís Premoli Azevedo (UVV); Fernando Luiz Tobias (UVV); Gilton Luiz Almada (UVV); Marcus Alexandre Vaillant Beltrame (UVV)

Resumo

The trade and consumption of shellfish in the region of Grande Vitória-ES is a fairly common practice in the capixaba culture, and among them, stands out the mussels. The mussels are bivalve mollusks that feed by filtering the water from where they live and therefore are considered marine bioindicators since they bioaccumulate pathogenic microorganisms in their tissues thereby causing food poisoning if consumed improperly. The objective of this study was to perform microbiological analysis of mussels sold in the cities of Vitória and Vila Velha, ES. For this, 32 samples of mussels (Perna perna and Mytella sp.) obtained from the local trade were analyzed. 100gr. of each sample were packed in plastic bags, stored in chilled isothermal boxes and sent to the Laboratory of Microbiology of the Veterinary Medicine Course at the Centro Universitário Vila Velha to perform the analysis. The tests were performed as described by Silveira, Junqueira e Silva (1997). Before the analytical removal an anti-sepsis of the hands was performed. With a brush the shells were rubbed under running water, removing waste attached to the bark, and then dried with paper towels. The shells were opened and 25 grams of the organisms, along with intervalvular liquid, were collected in a sterile bottle and then grinded. Decimal dilutions were made to make the analysis of total and fecal coliforms by the most probable number technique, standard plate counts for mesophilic microorganisms and search for Salmonella spp. were also realized. The study showed a high bacterial count of mesophilic (1.0x10⁴ to 7.5 x10⁶UFC/g) in municipalities demonstrating the deficiency in storage as noted. In Vila Velha, 10% of the samples showed growth of TC and FC in pre-cooked samples, and 10% of the rate samples in nature were contaminated by FC. Of the samples from Vitória, 58% showed a growth of TC and 25% of them were above the FC allowed. There was no growth of Salmonella spp. in the samples analyzed. Results showed the precarious conditions of hygiene in which the products are being marketed, requiring a more strict supervision by the competent bodies and an awareness of the traders with the adoption of good hygiene practices and preservation of the product.

Palavras-chave: mussel, Perna perna, microbiological analysis, contamination, bivalve

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