25º Congresso Brasileiro de Microbiologia

25º Congresso Brasileiro de Microbiologia

ResumoID:127-1

Área: Microbiologia de Alimentos ( Divisão K )

EVALUATION OF THE ROLES OF THE ENVIRONMENTAL FACTORS IN THE HUMAN GIT ON THE BEHAVIOUR OF PROBIOTIC CULTURES L. CASEI SHIROTA AND LC01 BY THE USE OF IN VITRO MODEL

Katia Gianni de Carvalho (USP); Monika Francisca Kruger (USP); Danielle Nader Furtado (USP); Svetoslav Dimitrov Todorov (USP); Bernadette Dora Gombossy de Melo Franco (USP)

Resumo

This study evaluated the influence of gastrointestinal environmental factors (pH, digestive enzymes, food components, medicaments) on the survival of Lactobacillus casei Shirota and Lactobacillus casei LC01, using a semi-dynamic in vitro model that simulates the transit of microorganisms through the human GIT. The strains were first exposed to different simulated gastric juices for different periods of time (0, 30, 60 and 120 min), and then to simulated intestinal fluids for zero, 120, 180 and 240 min, in a step-wise format. The number of viable cells was determined after each step. The influence of food residues (skim milk) in the fluids and resistance to medicaments commonly used for varied therapeutic purposes were also evaluated.

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The correct evaluation of possible interactions between medicaments and probiotic bacteria depends on the determination of MIC of these medicaments. The MIC for Spidufen, an anti-inflamatory and anti-rheumatic drug, was 40 mg/mL for both L. casei Shirota and L. casei LC01. Considering that the daily dose for this medicament is 600 mg, the MIC value associated to the volume of the human GIT indicate that the recommended daily dose will hardly affect the survival of the probiotic bacteria. More important are the medicaments for treatment of chronic diseases, such as Zocor, an anti-lipemic drug used for the reduction of the body lipids, Atlansil, an anti-arrhytmic drug normally used in long course treatments and Arotin, a drug from the group of the anti-depressants with neuroleptic effect, also used in long-term treatments, which presented MIC of 1.0 mg/ml, 1.25 mg/ml and 1.0 mg/ml, respectively. Due to their long-term application, they can accumulate in the gastrointestinal tract and affect the viability of the probiotic cultures.

Palavras-chave: GIT, LAB, Lactobacillus casei Shirota, Lactobacillus casei LC01, probiotic

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