| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1428-1 | ||||
Resumo:Dengue, Zika, and chikungunya are endemic arboviruses in Brazil and have significant relevance for human infection as they cause epidemics with a high incidence of cases, especially in the state of Goiás. Given the concurrent circulation of these arboviruses and the extensive infestation and spread of mosquito vectors in the region, the importance of viral surveillance in mosquitoes becomes evident in the context of vector control. This study aimed to detect the presence of dengue viruses (DENV 1-4), Zika virus (ZIKV), and chikungunya virus (CHIKV) in vectors mosquitoes in the municipality of Goiânia-Goiás, located in the Central-West region of Brazil. The Zoonosis department of the Municipal Health Secretariat of Goiânia captured mosquitos based on criteria such as neighbourhoods with a higher number of suspected cases and environmental characteristics considered favourable for vector proliferation. Mosquitoes were captured during the interepidemic period from June to December 2022 and subsequently sent to the laboratories of the Federal University of Goiás (BIOTEC/FF and LPI/IPTSP), where they were sorted into pools based on sex and species. Viral RNA was extracted from samples of salivary glands and midguts, and then tested in reverse transcription real-time polymerase chain reaction (RT-qPCR). A total of 402 specimens were processed and divided into 71 pools (60 ♀/11 ♂) comprising mosquitoes of the species Aedes aegypti, Aedes albopictus, Aedes fluviatilis, Aedes scapularis, and Culex sp. Nine pools (12.6%) tested positive for one or more arboviruses, with 66.7% of positive pools comprising non-engorged females and 33.3%, engorged females. Among the nine positive pools, 55.6% were from the species A. aegypti, 33.3% from Culex sp., and 11.1% from A. scapularis. ZIKV positivity was detected in 88.9% of the pools, identified in all studied species, while DENV-2 was detected in 11.1% of the A. aegypti pools. The identification of DENV-2 confirms the circulation of this serotype in the region. These findings revealed an increase within ZIKV infestation in Aedes spp., which can contribute to the planning of epidemiological surveillance measures with focus on investigating ZIKV infection in humans. Palavras-chave: Arbovirus, Mosquitoes, Detection, Surveillance Viral, Vector Control Agência de fomento:Programa de Pesquisa para o SUS - Ministério da Saúde, Fundação Oswaldo Cruz, CNPq, CAPES | |||||