Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 1399-1

1399-1

MICROBIAL COMMUNITY PROFILE OF MAIZE RIZOSPHERE CULTIVATED UNDER DIFFERENT PHOSPHATE FERTILIZATION CONDITIONS AND INOCULATED WITH A P-SOLUBILIZER INOCULANT

Autores:

Jonnathan Whiny Moraes dos Santos (EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA) ; Flavia Cristina dos Santos (EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA) ; Christiane Abreu de Oliveira-paiva (EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA) ; Eliane Aparecida Gomes (EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA) ; Ubiraci Gomes de Paula Lana (EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA) ; Isabela Figueiredo de Oliveira (UFSJ - UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL REI ) ; João Vitor Silvério Alves de Avelar (UFSJ - UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL REI ) ; Raquel Gomes de Oliveira (UFSJ - UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL REI ) ; Douglas Venâncio Alexandre da Silva (UNIFEMM - CENTRO UNIVERSITÁRIO DE SETE LAGOAS) ; Sylvia Morais de Sousa (EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA, UFSJ - UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL REI , UNIFEMM - CENTRO UNIVERSITÁRIO DE SETE LAGOAS)

Resumo:

The inclusion of bioinoculants within integrated nutrient management is growing due to their potential to increase plant growth and yield. However, it is necessary to determine the different conditions that effect commercial bioinoculant effectiveness. The aim of this work was to evaluate the effect of inoculation with BiomaPhos® and phosphate fertilization on microbial communities of maize rhizosphere. The maize genotype KWS 9006 Pro2 was evaluated in the Experimental Station of Embrapa Maize and Sorghum, in Sete Lagoas - MG, Brazil in the 2023 season. The experimental design was in a randomized complete block with three replicates. The treatments were with and without inoculation with the bioinoculant BiomaPhos®, composed of two P-solubilizing Bacillus strains (CNPMS B2084 and CNPMS B119), and three P-fertilizer treatments: without P-fertilizer addition (P0); Rock phosphate (RockP) or triple superphosphate (TSP) under three doses (0, 60,120 e 180 kg.ha-1) of P2O5. During the flowering time, non-rhizosphere and rhizosphere soil from three sorghum plants from each plot were collected and analyzed by terminal restriction fragment length polymorphism (T-RFLP). Total DNA extracted for 16S (for bacteria) and 28S rRNA (for fungi) genes was amplified and digested with three restriction enzymes AluI, HhaI and HaeIII. PCR digested products were genotyped on Genetic Analyzer 3500XL with GeneMapper 5.0 software and analyzed with T-REX program. The relative abundances of the microbial species were determined by the average T-RF size values of digestions with the restriction enzymes. For bacteria and arbuscular mycorrhiza fungi (AMF) there were significant differences between inoculated and non-inoculated treatments. The genetic diversity of the bacterial community differed significantly (p<0.05) between the inoculated and non-inoculated samples from the treatments without fertilizer and the treatments with 60, 120 and 180 kg.ha-1, as well as for the two sources of phosphates. The genetic diversity of the fungal community showed a significant difference between inoculated and non-inoculated samples only in treatments with a dose of 120 kg.ha-1 of the different types of fertilization treatment.. Our results expand the knowledge on microbial communities of maize under different doses and P fertilization conditions associated with inoculation.

Palavras-chave:

Arbuscular mycorrhiza fungi, Plant growth-promoting bacteria, T-RFLP

Agência de fomento:

Embrapa, CNPq, Fapemig and Finep