Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 1397-1

1397-1

EFFECT OF THE INOCULATION OF A P-SOLUBILIZER INOCULANT ON RHIZOSPHERE MICROBIOTA OF SORGHUM CULTIVATED UNDER DIFFERENT P FERTILIZATION CONDITIONS

Autores:

Isabela Figueiredo de Oliveira (UFSJ - Universidade Federal de São João del Rei ) ; Cícero Beserra de Menezes (EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária) ; Christiane Abreu de Oliveira Paiva (EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária) ; Eliane Aparecida Gomes (EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária) ; Ubiraci Gomes de Paula Lana (EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária) ; João Vitor Silvério Alves de Avelar (UFSJ - Universidade Federal de São João del Rei ) ; Raquel Gomes de Oliveira (UFSJ - Universidade Federal de São João del Rei ) ; Sylvia Morais de Sousa (EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária, UFSJ - Universidade Federal de São João del Rei )

Resumo:

The use of microbial inoculants is a technology that meets the precepts of ecological intensification of agriculture. BiomaPhos® is a commercial inoculant composed of two phosphate solubilizing bacterial strains (B119 and B2084), which promotes yield increase for different crops. This work aimed to evaluate the effect of different doses of BiomaPhos® on the genetic diversity of the rhizosphere microbiota of sorghum. The sorghum genotype BRS373 was evaluated in the Experimental Station of Embrapa Maize and Sorghum, in Sete Lagoas - MG, Brazil, in the 2022 and 2023 seasons. The experimental design was in a randomized complete block with three replications and seven treatments, T1: 0% P2O5, T2: 50% P2O5, T3: 100% P2O5, T4-T7: 50% P2O5 with 80, 100, 120 and 200 mL of BiomaPhos® for 200,000 seeds.ha^-1, respectively. During the flowering stage, rhizosphere and non-rhizosphere soil were collected from three sorghum plants from each plot and analyzed by terminal restriction fragment length polymorphism (T-RFLP). Total DNA was extracted and 16S (for bacteria) and 28S rRNA (for fungi) genes were amplified and digested with the restriction enzymes AluI, HaeIII and HpaII. PCR digested products were genotyped on Genetic Analyzer 3500XL with GeneMapper 5.0 software and analyzed with T-REX program. The relative abundances of the microbial species were determined by the average T-RF size values of digestions with the restriction enzymes. A significant difference on sorghum microbiota was observed in the bacterial communities in different P2O5 doses at Embrapa in 2022 and 2023 seasons (p<0.05). Moreover, for arbuscular mycorrhiza fungi (AMF) a significant difference on sorghum microbiota in different P2O5 doses was observed only at 2023 season (p<0.10). However, for bacteria and arbuscular mycorrhiza fungi (AMF) there were significant differences between inoculated and non-inoculated treatments only at 2022 season (p<0,05). Our results expand the knowledge on sorghum microbiota under different doses of bioinoculant and P fertilization conditions. Financial Agencies: Embrapa, Capes, CNPq, Fapemig and Finep

Palavras-chave:

Sorghum bicolor, T-RFLP, BiomaPhos, plant growth-promoting bacteria

Agência de fomento:

Embrapa, Capes, CNPq, Fapemig and Finep