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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 1302-1

1302-1

Effect of supplementation with Tryptophan and Glycerol on violacein production by Janthinobacterium lividum bacteria

Autores:
Nathan Aiko Hotta (UEL - Universidade Estadual de Londrina) ; Lucas Zanin Santos (UEL - Universidade Estadual de Londrina) ; Guilherme Biz (UEL - Universidade Estadual de Londrina) ; Elisete Pains Rodrigues (UEL - Universidade Estadual de Londrina)

Resumo:
Textile dyeing is one of the industrial activities that consume more water and is also one of the most polluting due to the use of synthetic dyes and other chemicals in the dyeing process. The development of alternative and sustainable technologies is necessary to minimize environmental impacts and conserve water. Biopigments produced by microorganisms, such as violacein, offer a sustainable alternative and can add relevant properties to the fabric, such as antimicrobial and antiviral activities. The pigment violacein is produced by the bacterium Janthinobacterium lividum, which is one of the most important producers of this pigment. Violacein has great potential for use in textile dyeing, particularly for silk fabrics. Therefore, the aim of this study was to evaluate the production of violacein in a medium supplemented with the amino acid tryptophan (a precursor for violacein synthesis) and/or glycerol as a carbon source. The strain of J. lividum was cultivated in nutrient broth medium supplemented with tryptophan and/or glycerol in a 2x2 factorial design. After 96 hours of growth under controlled agitation and temperature, both violacein production (measured by absorbance at 575 nm) and growth (measured by absorbance at 600 nm and 750 nm) were assessed. Violacein was extracted with ethanol, and after centrifugation, the extract was analyzed using spectrophotometry (at 575 nm). The data were analyzed using ANOVA and the Tukey test (0.05%) in the Jamovi program. The results showed that J. lividum produces violacein more efficiently when supplemented with both a carbon source and the precursor tryptophan. The production in an unsupplemented medium was the lowest. Additionally, the production in a medium supplemented with tryptophan did not differ significantly from that obtained in a medium with glycerol. The fermentation conditions established in this study will help in the development of more efficient violacein production processes for J. lividum, benefiting its application in the textile industry and for other sectors.

Palavras-chave:
 biopigmentos, corante, seda, sustentabilidade, tingimento


Agência de fomento:
Conselho Nacional de Desenvolvimento Científico e Tecnológico; SETI-PR – Seda Brasil Project.