| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1296-2 | ||||
Resumo:Enterobacter Huaxiensis (ACT-107) AND Enterobacter Asburiae (ACT-4) COLONIZING LEAF-FROGS OF THE SPECIES Phyllomedusa Distincta: GENOMIC DATA AND COMPARATIVE ANALYSIS
The Enterobacter genus is a polyphyletic taxon that includes genetically diverse species hard-to-identify by using phenotypic methods and antibiotic susceptibility patterns. We report E. asburiae and E. huaxiensis colonizing leaf-frog of the species Phyllomedusa distincta inhabiting the Brazilian Atlantic Forest. WGS analyses revealed the presence of blaACT-107 and blaACT-4 cAmpC genes in E. huaxiensis 4Pd9 and E. asburiae 4Pd7, respectively, responsible for resistance to penicillins, broad-spectrum cephalosporins, cephamycins and β-lactam/β-lactamase inhibitor combinations. Noteworthy, while phylogeographical analysis revealed that isolation of E. huaxiensis in Latin America has been from an animal host in Brazil, in 2017, at the same time of the first identification of the strain type from a human host in 2017, in China, SNP-based phylogenomic analysis using publicly available genome sequences from E. asburiae and E. huaxiensis isolated in Argentina, Colombia, Brazil, China and USA revealed unrelated clades. On the other hand, comparative resistome confirms intrinsic blaAmpC, fosA, oqxA and tolC genes among E. huaxiensis and E. asburiae species, whereas E. asburiae lineages acquiring blaKPC-2, blaOXA-2, blaOXA-9 or mcr-9 genes have emerged in Colombia and Argentina. In summary, our results confirm that: i) members of Enterobacter complex are ubiquitous species; ii) epidemiological investigation of genetically diverse species requires genomic methods; iii) novel AmpC variants could be related with specific Enterobacter species; and iv) clinically important emergent Enterobacter species can be originating from animal hosts.
Keywords: Enterobacter cloacae complex, AmpC, One Health, Phylogeographical analysis, Genomic surveillance. Palavras-chave: Enterobacter cloacae complex, AmpC, One Health, Phylogeographical analysis, Genomic surveillance | |||||