| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1294-1 | ||||
Resumo:The Brazilian Pantanal biome is known to harbor an enormous biodiversity, being a potential source of promising natural bioactive compounds to be studied. In a previous work, among 91 endophytic fungal isolates obtained from Vochysia divergens, a medicinal plant located in Pantanal region, the Xylaria arbuscula CMRP5059 strain stood out by showing promising bioactivity against the citrus phytopathogens Colletotrichum abscissum and Phyllosticta citricarpa. Here we aimed to characterize the bioactive secondary metabolites produced by the fungal strain CMRP5059 and evaluate its biological activity against two phytopathogenic fungi. To produce the secondary metabolites the endophytic fungi Xylaria arbuscula CMRP5059 was cultured in a large-scale fermentation in malt extract media, followed by solid phase extraction using XAD-16 resin and methanol, resulting in 7.7g of crude extract. This crude extract was subjected to various chromatographic techniques (e.g. Silica gel column, RP-18 reverse column, Sephadex LH-20, semi-prep HPLC), always followed by TLC and HPLC analysis. The generated fractions were submitted for biological activity evaluation against C. abscissum and P. citricarpa, as well as the cytotoxicity assay evaluation against A549 (non-small lung), PC3 (prostate) and HCT116 (colorectal) human cancer cell lines. Bioactive fractions were subjected for further purification, and the chemical structure of the generated pure compounds have been established by MS and 1D and 2D NMR. So far, over 30 compounds have been isolated and identified from this fungal crude extract, which belong to various chemical classes, including coumarins, peptides, terpenoids, cytochalasins. The purified compounds will be further evaluated regarding its antifungal activities, cytotoxicity and also used in other screening studies targeting antiviral and antibacterial activity by molecular docking. The endophytic fungi Xylaria arbuscula CMRP5059 has been proved to be a great producer of a large range of bioactive natural products that can be useful against phytopathogenic fungi, highlighting the Brazilian Pantanal biome as a promising source of endophytic microrganisms with biotechnological potential. Palavras-chave: Pantanal, Endophytic fungi, Vochysia divergens, Phytopathogens Agência de fomento:CAPES, CNPq, Fundação Araucária | |||||