Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 1291-1

1291-1

HUMAN NASOCOMIAL CLONE OF VIM-2-PRODUCING PSEUDOMONAS AERUGIONSA ST233 INFECTING A DOG

Autores:

Guilherme Emanuel Pereira de Paiva (USP - UNIVERSIDADE DE SÃO PAULO) ; Fabio Parra Sellera (USP - UNIVERSIDADE DE SÃO PAULO, UNIMES - UNIVERSIDADE METROPOLITANA DE SANTOS) ; Fernanda Panseri Rodrigues (ANCLIVET - ANCLIVET LABORATÓRIO VETERINÁRIO) ; Anderson Cleiton de Souza (ANCLIVET - ANCLIVET LABORATÓRIO VETERINÁRIO) ; Fernanda Esposito (USP - UNIVERSIDADE DE SÃO PAULO) ; Nilton Lincopan (USP - UNIVERSIDADE DE SÃO PAULO)

Resumo:

Carbapenemase-producing bacteria have no longer been restricted to human healthcare settings and have begun to be identified in non-human hosts. Otitis is a common disease that affects companion animals worldwide. Multidrug-resistant Pseudomonas aeruginosa isolates have been responsible for difficult-to-treat otitis in dogs. An ear sample from a dog suffering from otitis externa was collected through a sterile swab and subjected to bacterial culture. One P. aeruginosa isolate was recovered and identified by Matrix-assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF). Antimicrobial susceptibility testing was performed using the following human and veterinary important antimicrobials: amikacin (30), aztreonam (30), cefepime (30), ceftolozane/tazobactam (40), ciprofloxacin (5), gentamicin (10), imipenem (10), meropenem (10), piperacillin/tazobactam (110), ticarcillin/clavulanic acid (85). Surprisingly, the P. aeruginosa strain displayed resistance to all antimicrobials tested, except for aztreonam. Genomic DNA was sequenced using the Illumina NextSeq platform, and in silico prediction was analyzed by bioinformatics tools. The P. aeruginosa strain was found to carry several resistance genes, including blaVIM-2, blaOXA-486, and blaPAO, which confer resistance to β-lactams; catB7, responsible for chloramphenicol resistance; aac(6')-II and aac(3')-Id, responsible for aminoglycoside resistance; dfrB5, responsible for trimethoprim resistance; and fosA, responsible for fosfomycin resistance. Multilocus sequence typing (MLST) analysis revealed that this strain belonged to sequence type ST233, which has been associated with human nosocomial infections and high mortality rates in several countries. The discovery of this human-associated lineage of VIM-2-producing P. aeruginosa beyond hospital settings is concerning, indicating its spread to small animal practice. These findings shed light on the emergence of this resistant clone and highlight the importance of monitoring antimicrobial resistance in both human and veterinary medicine to prevent further dissemination and ensure effective treatment options.

Palavras-chave:

CARBAPENEMASE, ONE HEALTH, PSEUDOMONAS AERUGIONSA

Agência de fomento:

CNPq