| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1284-1 | ||||
Resumo:Salmonella enterica is a leading foodborne bacterial pathogen that causes considerable public health burden, whit the primary transmission route to humans being the consumption of contaminated food, particularly animal-derived products such as eggs, unpasteurized milk, and poultry meat products. In this regard, even though most salmonellosis usually presents as self-limiting gastroenteritis, severe invasive infections may occur with enhanced morbidity and mortality among young children, elderly, and organ transplanted recipients. In 2021, a 45 years-old kidney transplant recipient woman was admitted to a Teaching Hospital at University of Campinas, Brazil, presenting with persistent acute diarrhea in association to diffuse abdominal pain, nausea, weakness, and vomiting symptoms, while denied fever. Fecal samples collected upon admission resulted in the identification of an extended-spectrum beta-lactamase (ESBL)-producing Salmonella spp. Bacterial identification and antimicrobial susceptibility testing were performed using MALDI-TOF/MS and the Kirby-Bauer method, respectively. Genomic DNA was obtained using PureLink Extraction Kit and sequencing performed on an Illumina NextSeq550 platform. De novo assembly was performed using Unicycler and in silico analysis performed using bioinformatic tools. Plasmid contigs were assembled with PlasmidSpades and gaps closure performed using Bandage and Geneious Prime R10 software. The S. enterica (Sal800 strain) displayed resistance to broad-spectrum cephalosporins including aztreonam, ceftriaxone, cefotaxime, ceftazidime, and cefepime. Multilocus sequence typing (MLST) and serovar prediction assigned Sal800 strain to sequence type (ST) ST2015 and to the rare serotype Coeln, which has been previously identified causing foodborne outbreaks linked to minced beef in France, and imported pre-washed ready-to-eat salad in Norway, whereas in Brazil, it has been previously isolated from human and poultry sources. The blaCTX-M-8 gene was found to be flanked downstream to ΔIS10-IS26 and upstream by a IS26 genetic context that was inserted onto an IncI1 conjugative plasmid of ~ 90 kb belonging to plasmid sequence type (pST) pST113. The plasmid was successfully transferred to Escherichia coli J53 recipient strain by conjugation. Noteworthy, the IncI1-pST113 carrying blaCTX-M-8 was confirmed as an epidemic plasmid identified in different ecological niches, including humans, poultry meat, companion animals, healthy cattle and wild animals with worldwide distribution. These findings highlight the expansion of epidemic plasmid to foodborne pathogens with One Health implications. Palavras-chave: foodborne pathogen, antimicrobial resistance, conjugative plasmid, one health Agência de fomento:Bill & Melinda Gates Foundation (Grand Challenges Explorations Brazil OPP1193112) | |||||