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Congresso Brasileiro de Microbiologia 2023
Resumo: 1267-1

1267-1

ASSESSING OF THE EFFECTS OF PHYLLOSTICTA CITRICARPA ON THE BIODEGRADATION OF HERBICIDES

Autores:
Biana Pelissari Gadanhoto (IAC - Instituto Agronômico de Campinas) ; Fernando Alves de Azevedo (IAC - Instituto Agronômico de Campinas) ; Renato Nallin Montagnolli (UFSCAR - Universidade Federal de São Carlos)

Resumo:
Biodegradation consists of the elimination of organic compounds in an ecosystem by the metabolic activity of the organisms present in that place, as well as the local microbiota. When complete mineralization of contaminants occurs, the process results in carbon dioxide and water. Understanding the scenario and the first microbial interactions when the relationship between environment and contaminant begins is a way to bring answers that the study of the kinetics of biodegradation of pesticides provides. The aim of the present study is to evaluate the remedial potential of Phyllosticta citricarpa and how environmental samples are affected by monitoring microbial activity. In this research, Phyllosticta citricarpa was added to biodegradation tests containing the herbicides Oxyfluorfen and Flumioxazin, which was monitored by a respirometric technique capable of evaluating the production of CO2 and microbial activity through the application of the redox indicator 2,6-DCPIP. It was observed that the biodegradation of contaminants by the action of soil microorganisms and the presence of Phyllosticta citricarpa generated a greater production of CO2 and consequent biodegradation, mainly in the test of the herbicide Oxyfluorfem (852.28 mg of CO2). The discoloration tests reaffirm the biodegradation results of respirometry, where there is the process by the local microbial community of the soil (12 and 15 hours), since the sample comes from an agricultural area and citrus culture, which is the same recommended for the study herbicides. However, it presents higher biodegradation values when added to the study inoculum (7 and 8 hours). Herbicides are major sources of carbon to be degraded, but it is not always bioavailable, so when the inoculum is added it occurs more quickly, therefore the presence of Phyllosticta citricarpa extract is effective against the negative effects in environmental scenarios.

Palavras-chave:
 2,6-Dichloroindophenol, Biorremediation, Herbicides, Respirometry


Agência de fomento:
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001