| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1242-1 | ||||
Resumo:Bacupari-do-Cerrado (Salacia crassifolia) is a fruit from Cerrado Savannah with the potential for economic exploitation due to its high nutritional and functional value. During this fruit processing, by-products are generated and represent an important source of bioactive compounds and can also be studied for the presence of microorganisms with properties related to probiotic activity. Our study group has isolated lactic acid bacteria from the seeds and peel of this fruit, which has previously been identified by 16 sRNA as Lactiplantibacillus plantarum/pentous. To ensure the probiotic potential of this strain, in vitro assays are needed, therefore this study aimed to confirm the specie and its proprieties associated with probiotic effects. The whole genome of the strain was sequenced using Illumina MiSeq® System. For the essays, the strain was activated three times in De Man, Rogosa and Sharpe (MRS) broth at 37 °C for 24 h and under anaerobiosis conditions. Its capacity to degrade mucin was tested to confirm the strain's safety aspect. Also, the physiological functionality properties of autoaggregation and antagonistic activity against pathogens Listeria monocytogenes (ATCC 19111) and Bacillus cereus (ATCC 11778) were evaluated. Finally, the strain capacity to produce diacetyl, proteolytic and lipolytic activity was tested. All assays were performed in triplicate in three independent experiments and the results were expressed as the average of the obtained data. The strain was confirmed as L. plantarum by whole genome sequencing and it was incapable of degrading mucin, as recommended safety requisites for probiotics. Regarding the physiological functionality properties, the strain was able to autoaggregate (45,76% ± 0,1), meaning the highest barrier effects to prevent the colonization of pathogens in intestinal microbiota; and presented inhibitory effects against L. monocytogenes, related to the production of inhibitory substances, an important functional property of probiotics; but not against B.cereus. Positive results for proteolytic activity were observed, indicating a control factor playing a major role in cellular and physiological processes; but negative for diacetyl production and lipolytic activity. These results indicate that Lactiplantibacillus plantarum strain isolated from the Bacupari-do-Cerrado by-product could present potential probiotic properties that could be potential candidates for probiotic application for human health. Further, in vivo studies are needed to continue the contribution to scientific advances in new probiotic strains and contribute to environmental sustainability and regional Cerrado Savannah appreciation.
Development Agency: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Palavras-chave: Cerrado biome, Fruit by-product, Microbiota, Whole genome sequencing Agência de fomento:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |||||