Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 1187-2

1187-2

MICROBIOLOGICAL, CHEMICAL, AND ISOTOPIC ANALYSES OF SOIL UNDER DIFFERENT LAND USES IN THE ATLANTIC FOREST/CERRADO ECOTONE

Autores:

Franciele Muchalak (CENA-USP - CENTRO DE ENERGIA NUCLEAR NA AGRICULTURA, UNIVERSITY OF SÃO ) ; Solange dos Santos Silva Zagatto (CENA-USP - CENTRO DE ENERGIA NUCLEAR NA AGRICULTURA, UNIVERSITY OF SÃO ) ; Luís Felipe Guandalin Zagatto (CENA-USP - CENTRO DE ENERGIA NUCLEAR NA AGRICULTURA, UNIVERSITY OF SÃO ) ; Gabriel Gustavo Tavares Nunes Monteiro (CENA-USP - CENTRO DE ENERGIA NUCLEAR NA AGRICULTURA, UNIVERSITY OF SÃO ) ; Guilherme Lucio Martins (CENA-USP - CENTRO DE ENERGIA NUCLEAR NA AGRICULTURA, UNIVERSITY OF SÃO ) ; Anderson Santos de Freitas (CENA-USP - CENTRO DE ENERGIA NUCLEAR NA AGRICULTURA, UNIVERSITY OF SÃO ) ; Tsai Siu Mui (CENA-USP - CENTRO DE ENERGIA NUCLEAR NA AGRICULTURA, UNIVERSITY OF SÃO ) ; Acacio Aparecido Navarrete (UB - Universidade Brasil)

Resumo:

An ecotone refers to the transitional region between two distinct ecosystems, where characteristics of both blend and present high biodiversity. The existence of ecotones is crucial for gene flow and for maintaining biodiversity, as they act as ecological corridors between different habitats. These locations are especially sensitive to environmental changes and are indicative of ecosystem health. The aim of this study was to investigate and correlate specific soil parameters in an Atlantic Forest/Cerrado ecotone region. Soil samples were collected from three different areas: a native forest with ecotonal characteristics, a pasture, and an area under ecological restoration process. Using real-time quantitative PCR (qPCR), the abundance of Bacteria, Archaea, and Fungi communities was quantified from the genomic DNA isolated from the soil samples. Analyses of acid phosphatase, nitrogen and carbon isotopes, and the determination of soil chemical parameters were carried out to gain a more comprehensive understanding of soil properties. Spearman correlation analysis was applied to evaluate the interrelationships between microbiological, chemical, and isotopic soil parameters examined in different areas. The results showed significant relationships between the abundance of Archaea, bacteria, and fungi in the soil and the chemical and isotopic parameters, pointing to complex and multifaceted interactions between microbial community abundance and soil environment. Acid phosphatase showed positive correlations with elements like organic matter (0.45), aluminum (0.58), and carbon (0.66), and negative correlations with pH (-0.51) and δ13C (-0.57). The abundance of the soil fungal community showed positive correlations with aluminum content (0.33) and aluminum saturation (m (%)) (0.27), and negative correlations with levels of calcium (-0.27) and magnesium (-0.35). Regarding the microbial communities, the abundance of the soil bacteria showed positive correlations with levels of organic matter (0.25), iron (0.24), and total organic carbon (TOC) (0.25), while the abundance of the Archaea community exhibited positive correlation with potassium content (0.32) and δ13C (0.23) and negative correlation with cation exchange capacity (CEC) (-0.23). These results provide an overview of the interactions between microbiological, chemical, and isotopic soil factors, offering insights for soil management in conservation practices.

Palavras-chave:

Ecotone, Atlantic Forest, Cerrado, qPCR, soil properties