| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1181-1 | ||||
Resumo:Fusarium is a genus of filamentous fungi that have emerged as major opportunistic fungal agent, mycotoxin producer and agronomically important plant pathogen. Since Fusarium spp. typically show broad resistance to antifungal drugs and can lead complications for immunocompromised patients and economic problems in crops, diversity identification at the species level and the investigation of activity against antifungals are required for better comprehension of these species and their epidemiological distribution in the environments. This work is part of LatAsp Project (www.latasp.com), which citizen volunteers were recruited for air sampling during 2022 to 2023, in several places of Latin America. So, stickers were exposed to air for 10h, incubated 24h on Sabouraud dextrose agar medium plus chloramphenicol, and the colonies of Fusarium were isolated by microscopic identification of conidiogenous cells and cultured on Sabouraud dextrose agar medium. Initially, 47 Fusarium spp. were included in this study, DNA extraction was performed for molecular identification by sequencing of the translation elongation fator 1 alpha (TEF-1 α) and internal translation spacer (ITS) regions. Of this total, 34 isolates were sequenced and analysed by BLAST in NCBI (https://blast.ncbi.nlm.nih.gov/) and Fusarium ID (https://www.fusarium.org/) databases. Fusarium isolates were identified to six species complexes: Fusarium solani specie complex (FSSC, n=15), Fusarium fujikuroi species complex (FFSC, n=10), Fusarium oxysporum species complex (n=4), Fusarium incarnatum-equiseti species complex (FIESC, n=2), Fusarium tricinctum species complex (FTSC, n=2) and Fusarium chlamydosporum species complex (FCSC, n=1). Some divergences among species were found between databases, phylogenetic analysis in relation to reference isolates were done and some isolates kept classified at level of species complexes. Most of isolates was found in southeast of Brazil (88,2%), mainly in park regions (52,9 %) in relation to urban (44,1 %) and rural (2,9 %) areas. The in vitro susceptibility activities showed high MICs for eight antifungals tested. AMB (amphotericin B) appeared to be the most active antifungal, ranging between 1 and 4 ug/mL. Isolates from F. fujikuroi specie complex had more variability for MCZ (miconazole), 2 to >16 ug/mL. Most of the isolates had MICs of > 16 ug/mL for ITC (itraconazole) and >8 ug/mL for VRC (voriconazole), showing high resistance to antifungal drugs and the importance to study the widespread of these different species into Brazil. Palavras-chave: molecular identification, resistance, air sampling, species complexes, fungi Agência de fomento:U.S. Centers for Disease Control & Prevention, Antimicrobial, Resistance Solutions Initiative | |||||