| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1170-1 | ||||
Resumo:SARS-CoV-2 (cause of COVID-19) has great clinical relevance in public health. Now, vaccination is still the only way to prevent coronavirus infection. Thus, many efforts have been directed to search for products with virucidal activity to control the viral spread and reduce the number of infections. Several researchers have sought new nanomaterials with anti-SARS-CoV-2 properties that can be used in the production of personal protective equipment (PPE) and disinfectants. Interestingly, silver-based materials have been widely used in different industrial applications, because of their physicochemical and biological properties such as antifungal, antibacterial, and antiviral. However, silver is a precious metal, and due to this silver-based materials have a higher cost of production. Here, we evaluated the anti-SARS-CoV-2 activity of tungstate (WO4) microcrystals combined with the silver under different shapes (cube, nano, rod, and membrane containing chitosan-encapsulated silver tungstate particles using at 0.25%, 0.5%, 1% or 2% of concentrations.), or combined with non-precious metals (iron, copper, zinc, and nickel). In addition, we also examined the antiviral effect of molybdate (MoO4) microcrystals combined with the silver, iron, copper, zinc, or nickel. For this, the nanoproducts at different concentrations were incubated with DMEM medium containing SARS-CoV-2 at 1.5x104 PFU/mL. After 5 and 30 min of interaction, the supernatants were collected, and the virus replication was determined by quantification of the number of plaque-forming units (PFUs). Unencapsulated α-Ag2WO4 in chitosan, independent of shape (cube, nano, and rod), inhibit more than 80% of SARS-CoV-2 replication at 5 min and 30 min. In contrast, chitosan encapsulation resulted in inhibiting of 30% and 60% at 5 and 30 min, respectively. Tungstate or molybdate combined with other metals showed a dose-dependent antiviral activity inhibiting more than 80% at 2% and 1% concentration. However, zinc tungstate microcrystals have no anti-SARS-CoV-2 effect in any concentration. Our preliminary data suggests that the shape and the time do not affect the action of silver tungstate on SARS-CoV-2 replication. However, the encapsulation of the compounds in chitosan promotes a decrease in their biological activity. It is possible that the loss effect was because of the change of surface energy of microcrystals promoted by chitosan. Furthermore, we observed that silver particles have a higher inhibition activity when compared with iron, copper, and zinc particles. Palavras-chave: antiviral activity, molybdate, nanomaterial, SARS-CoV-2, Tungstate Agência de fomento:INOVA-FIOCRUZ; IOC-FIOCRUZ; VPPIS-004-FIO-22-2-21; FAPERJ; CNPq; CAPES | |||||