| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1154-1 | ||||
Resumo:Many animal species have acquired synanthropic behavior, and thereby may have a role in the transmission of zoonotic diseases. Among the group of synanthropic animals, some species of bats (order Chiroptera) are extremely adapted to large urban centers, where they find water and shelter. Some of the characteristics that contribute for them to be important disseminators of pathogens. Therefore, the aim of this study was to evaluate the occurrence of two bacterial zoonotic pathogens (Salmonella spp. and Staphylococcus aureus) using the Polymerase Chain Reaction (PCR) technique in liver samples from bats from the Mammals Collection of the Federal University of Lavras (Coleção de Mamíferos da Universidade Federal de Lavras - CMUFLA). In this study, samples of 144 specimens were used, representing 13% of the CMUFLA; they were randomly selected, only from bat species with synanthropic behavior. All specimens were preserved in alcohol 70%, from which liver samples were collected. The DNA extraction was performed using the kit “Genomic DNA Purification Kit (Wizard®). The DNA samples were used to assess the presence of the ompc gene (159 bp) for Salmonella spp. and the nuc gene (400bp) for S. aureus using the PCR technique. The 144 assessed specimens included 51 species, 37 genera and 7 families of bats. Males were more frequent [84/144 (58.3%)] than females [38.2% (55/144)], and 3.5% (5/144) of the samples gender were not identified. Regarding the place of origin, the specimens were from 22 municipalities located in the states of Minas Gerais, São Paulo, Pará, Bahia, Goiás and Tocantins. The DNA of Salmonella spp. was detected in 2.8% (4/144) of the individuals: one of Tonatia bidens species [Phyllostomidae family, male, from the municipality of Santa Maria da Vitória (BA)]; one of the species Sturnira lilium, [Phyllostomidae family, male, from Parque Estadual do Ibitipoca in Lima Duarte (MG)]; one of the species Promops nasutus [Molossidae family, female, from Parque Estadual do Rio Doce in Marliéria (MG)]; and one of the species Molossus aztecus [Molossidae family, male, from the same locality (Parque Estadual do Rio Doce) was also positive. The DNA of S. aureus was not detected in the samples. Therefore, the detection of Salmonella spp. in bats even with low prevalence indicates the potential of these animals to be carriers of this pathogen, which shows the relevance of studies with these synanthropic animals in the context of One Health. Palavras-chave: One health, PCR, Salmonella spp., Staphylococcus aureus, zoonose Agência de fomento:CAPES, FAPEMIG, CNPQ | |||||