| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1111-1 | ||||
Resumo: Intoduction:Urinary tract infections (UTIs)caused by Escherichia coli are the most common types of infection, considered the second most common outpatient infection behind only respiratory tract infections. UTIs are more prevalent in women in the second and third decades of life, from 15 to 29 years old. In recent years, variations in antimicrobial susceptibility have been observed worldwide, because of progressive resistance to the treatment of these infections. The choice of antimicrobial must agree with epidemiological data and, currently, some guidelines recommend the use of Fosfomycin for cases in which local resistance rates of pathogens causing acute UTIs are not above 20% or in cases of uncomplicated UTIs. Objective:This study aimed to evaluate the resistance profile of E. coli to Fosfomycin through the analysis of urine cultures in a private laboratory in a northeastern capital, from 2021 to 2023. Methods: An observational and cross-sectional study of biochemical samples of E. coli in private laboratory in the city of São Luís, Maranhão. Cultures from the period from 2019 to 2023 were included. The identification of isolated microorganisms was performed using mass spectrometry with the Microflex-Bruker Daltonics/BioTyper™ (MALDI-TOF) equipment (Bruker Daltonics GmbH and Co. KG - Bremen, Germany). The equipment was calibrated initially with the Bruker Bacterial Test Standard (BTS) as recommended by the manufacturer. To determine the susceptibility profile of the isolated bacteria in relation to antimicrobials, the Phoenix-BD equipment (Becton and Dickinson, Franklin Lakes, NJ, USA) and automated system Vitek2-CC4 (bioMérieux, Inc., Durham, NC, USA) with the AST-YS07 cards were used. For the polymyxin susceptibility test, the broth microdilution technique was employed to determine the minimum inhibitory concentration. For carbapenem-resistant microorganisms, the resistant test from automated system was confirmed using the Muller-Hinton and E-Test® bioMerieux gradient strips to determine the minimum inhibitory concentration (MIC). The results were interpreted based on the readings obtained from the cutoff points and analyzed according to the parameters established by the Brazilian Committee on Antimicrobial Susceptibility Testing (BrCast)/European Committee on Antimicrobial Susceptibility Testing guidelines. Results: The total number of samples analyzed was 8.362 of which 3.815 were outpatients (45,62%) and 4.547 (54,37%) were hospitalized. The female gender was responsible for most of the isolates, either in hospital or outpatient infections, with 87,8% of the total samples. In outpatients, it showed sensitivity in 3.696 (96,88%). Among the hospital samples, there was sensitivity in 96,98% of the tested samples. Conclusion: Fosfomycin proved to be a drug with good antimicrobial activity against E. coli strains, being considered a good therapeutic option.
Keywords: Bacterial urinary tract Infection, Escherichia coli, Fosfomycin.
Palavras-chave: Bacterial urinary tract Infection, Escherichia coli, Fosfomycin | |||||