| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1094-1 | ||||
Resumo:Since the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) infections in 2019, COVID-19 infection has promptly spread worldwide leading to over hundreds of millions diseased individuals and over 6,000,000 million deaths. It is well accepted that microbiota may overall influence disease pathogenesis and its modulations in different physiological conditions are among the most important questions in modern medicine related to diagnosis, treatment and prognosis. Besides, it is being discussed that viruses tend to interact with host local physiology resulting in microbial community modulations in the upper and lower respiratory tracts. To date, little is known on nasopharyngeal microbiota in patients with COVID-19. This study aimed to comparatively evaluate microbiota structure from nasopharyngeal secretion and epidemiological characteristics of community (n=40) or hospitalized SARS patients due to COVID-19 (n=40) and community SARS patients due to other etiologies (n=40). The research protocol was evaluated and approved by ethics committee at Universidade Federal de Juiz de Fora (UFJF). From nasopharyngeal secretion of patients attended to a COVID-19 diagnosis service at UFJF, microbiota fingerprints were obtained by REP-PCR after metagenomic bacterial DNA isolation. REP-PCR reactions were performed using a single primer (GTG)'5. Amplicons were separated by polyacrylamide gel electrophoresis (8% w/v), and an UPGMA dendogram based on the presence or absence of amplicons were produced using Jaccard's coefficient and bootstrap of 1000x. Epidemiological characteristics for every patient included was observed. According to the medical records, risk factors for disease severity was markedly predominant in COVID-19 positive hospitalized individuals (> 80%). Gender and age were evenly distributed. Cough were commonly related although fever, dyspnea, headache, pain, O2 saturation < 95%, anosmia and ageusia were more related to COVID-19 patients both community and hospital admitted. Microbiota fingerprints were evaluated by the hierarchical agglomerative method and three clusters were identified. Group C1 was composed mainly by COVID-19 hospitalized SARS patients (97,4%) whereas group C2 was composed mainly by community SARS patients due to COVID-19 (82,5%) and community SARS patients due to other etiologies (84,6%). The data obtained so far is being evaluated by multiple correspondence analysis approach. The data is of relevance and may confirm the findings that upper respiratory microbiota could play important role in the severity of respiratory viral infections. Thinking beyond COVID-19, further prospective studies are needed to better address the phenomenon to understand how such microbial patterns may impact these infectious diseases or, in turn, weather this is an ecological consequence of environmental changes related to virus-host-microbiota interactions. Palavras-chave: COVID-19, Pacientes comunitários, Pacientes hospitalizados, Síndrome Respiratória Aguda Grave, Microbiota trato respiratório superior Agência de fomento:FAPEMIG, CNPQ, CAPES, UFJF | |||||