| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1052-1 | ||||
Resumo:Leptospirosis is a neglected zoonosis caused by bacteria of the genus Leptospira, and affects domestic and wild animals, as well as humans. Leptospira santarosai from Sejroe serogroup, serovar Guaricura, is recognized as an important agent of Bovine Genital Leptospirosis (BGL), a chronic and silent disease that causes reproductive disorders such as abortions, stillbirths, estrus repetition, and economic losses. Despite clinical signs related to reproductive failure, most studies have focused on the detection of viable leptospires or their DNA in urine. As such, little is known about the pathophysiology of leptospires in the reproductive tract and there is a lack of information about important aspects of BGL. Based on this, the aim of the present study was to perform whole-genome sequencing (WGS) of three strains from L. santarosai Sejroe serogroup - 2013_VF52, 2014_VF66, and VF237- isolated from the vaginal fluid of cows with reproductive injuries from Rio de Janeiro, Brazil. Additionally, the strain 2014_U76 (L. santarosai Sejroe serogroup), previously isolated from the urinary tract of a cow was also submitted to WGS. High-quality genomic DNA was obtained from cultures deposited in the Collection of Bacterial Cultures of Veterinary Interest (Federal Fluminense University, Brazil). DNA was extracted and whole-genome sequencing was performed on Illumina MiSeq platform using a Nextera DNA Flex Kit. Genomes were deposited on GenBank under accession numbers JAUOTG000000000, JAUOTF000000000, JAUOTH000000000 and JAUOTE000000000. Reads were quality-trimmed using the software Trimmomatic 0.39, and all four genomes were assembled again using the Unicycler 0.4.8 workflow. Prokka was applied for the genome annotation, contemplating the CDS, tRNA, and rRNAs prediction. The genomes were aligned and compared according to 2 reference genomes (L. interrogans Fiocruz L1-130 and L. santarosai LT 821). A search for important genes was carried out in the genome. Among the 30 genes analyzed, 6 genes were present in all 4 samples: Loa22, LipL41, LipL32, LipL45, LipL21 and pbpB. Five of these genes are antigenicity and virulence genes (Loa22, LipL41, LipL32, LipL45, LipL 21), and one of them is a resistance gene (pbpB). Analyzing the divergences, 2 genes were found only in strains 2014_U76 and 2014_VF66: ponA (penicillin resistance gene) and ompL 37 (antigenicity gene). The results presented here are preliminary but may elucidate importants aspects in the pathophysiology of BGL. Palavras-chave: WGS, bovine leptospirosis, bioinformatics Agência de fomento:FAPERJ | |||||