Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 1051-2

1051-2

ISOLATION AND SCREENING OF L-ASPARAGINASE PRODUCED BY FUNGI ISOLATED FROM ANTARCTIC SOILS

Autores:

Averlane Vieira da Silva (UFAL - UNIVERSIDADE FEDERAL DE ALAGOAS) ; Jessia Elem Cunha Barbosa (UFAL - UNIVERSIDADE FEDERAL DE ALAGOAS) ; Mariana dos Santos (UFAL - UNIVERSIDADE FEDERAL DE ALAGOAS) ; Kelly Fernanda Seára da Silva (UFAL - UNIVERSIDADE FEDERAL DE ALAGOAS) ; Aline Cavalcanti de Queiroz (UFAL - UNIVERSIDADE FEDERAL DE ALAGOAS) ; Magna Suzana Alexandre Moreira (UFAL - UNIVERSIDADE FEDERAL DE ALAGOAS) ; Alysson Wagner Fernandes Duarte (UFAL - UNIVERSIDADE FEDERAL DE ALAGOAS)

Resumo:

Extremely cold environments such as Antarctica harbor microorganisms that live on the edge of life. Such microorganisms, including fungi, are both native and exogenous, many still unknown, and for this reason are targets of research interest with important biotechnological applications. An example of such an application is the enzyme L-asparaginase (EC 3.5.1.1, ASNase) considered a biopharmaceutical that catalyses the hydrolysis of L-asparagine (Asn) into L-aspartic acid and ammonia. Currently, ASNase is the only asparagine metabolism-directing agent used clinically, for the chemotherapeutic treatment of childhood acute lymphoblastic leukaemia (ALL) and lymphoblastic lymphoma. In this sense, it was aimed to study an enzyme from Antarctic cold-adapted eukaryotes (fungi) that may have potential applications in the production of L-asparaginase. The fungi used in this study were recovered from Antarctic soils. The strains were isolated on selective medium and preserved in 20% glycerol at -80.0 °C. Primary analysis (screening) of fungi was done using modified Czapek Dos (MCD) medium in triplicate with pH adjusted to 5.5 using four types of substrates (nitrate, asparagine, glutamine and urea) supplemented with phenol red and plates were incubated at 15.0 °C. Colony diameter and production zone diameter were calculated by measuring the inner diameter of fungal growth and enzyme production, respectively. A total of 149 were recovered, of which 37 were positive on selective screening. Ten isolates had better enzyme production indices in the initial screening. After 7 days of incubation the maximum index value was observed to be 5.45, 4.74 and 4.49 for three isolates producing L-asparaginase and glutaminase, urease free and 4.48 for one yeast producing asparaginase and glutaminase urease free. Moreover, these initial results suggest that fungi from Antarctica may lead to an important advance in therapeutic applications with fewer adverse effects, since the production of the urease-free enzyme was observed.

Palavras-chave:

Biopharmac, Selective insulation, Biotechnological application

Agência de fomento:

A Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)