| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1049-2 | ||||
Resumo:The search for sustainable agricultural practices has become increasingly relevant in the face of global challenges related to food security and environmental preservation. In this context, the use of beneficial microorganisms, such as the bacterium Azospirillum brasilense, has gained prominence as a promising strategy to increase agricultural productivity in a sustainable way. In addition, the use of materials such as biochar and activated coals has been evaluated to improve soil properties and provide an environment conducive to microbial growth. Therefore, the objective was to evaluate the growth and survival of A. brasiliense (Ab-V5) over 90 days, by counting colony-forming units (CFU). There were 12 treatments for biochar (BC) and activated coal (ACI and ACII), each treatment could have or not hydrochloric acid (HCl) or glycerol (GL). The biochar of macaúba (Acrocomia aculeata) was divided into different treatments T1 = BC + HCL + GL; T2 = BC + HCL; T3 = BC + GL; T4 = BC, 4 treatments for ACI treatments T5 = ACI + HCL + GL; T6 = ACI + HCL; T7 = ACI + GL; T8 = ACI and 4 treatments for ACII T9 = ACII + HCL + GL; T10 = ACII + HCL; T11 = CAII + GL; T12 = ACI. The polypropylene plastic bags (12 X 10 cm) were sterilized, homogenized and stored at 25 °C until use. The Ab-V5 strain was multiplied in nutrient broth, incubated at 28 °C for 48 hours and the bacterial suspension was adjusted to 109 CFU/mL-1 and injected into macaúba-based formulations in polypropylene plastic bags. The counting of the 90 days began 24 hours after inoculation.The data were submitted to analysis of variance and the comparison of means was made by Tukey's test at 5% significance, using the SISVAR® software. The biochar did not affect the survival of Ab-V5 on day 0, however with the passage of the incubation time it was possible to observe a positive interaction between the biochar and Ab-V5 on 30 days, there was a maximum value in the growth curve of the bacterium on day 45 for T1 that presented the value of 109 CFU/g-1, superior to other treatments, maintaining better survival. Regarding ACI and ACII coals, the interaction between these coals and the bacteria was less pronounced compared to BC. In the case of ACI coal, the highest bacterial growth occurred on day 15, and for T6 the highest occurred on day 0, both with a maximum value of 107 CFU/g-1. In the treatments T7 and T8, there was not bacterial growth. In ACII coal the bacterial highest occurred on day 90 for T9 and T10 the highest occurred on day 15, both with the value of 106 CFU/g-1. For the other treatments, there was not bacterial growth. Biochar has been shown to promote bacterial survival, evidencing its usefulness as a viable carrier, while activated coals showed less efficacy in this aspect. Palavras-chave: Agricultural, bacterial carrier, Ab-V5, biofertilizer, shelflife Agência de fomento:Ministério da Agricultura e Pecuária - MAPA - BRAZIL | |||||